Comparison of annulus fibrosus cell collagen remodeling rates in a microtissue system

It has been suggested that curvature progression in adolescent idiopathic scoliosis occurs through irreversible changes in the intervertebral discs. Strains of mice have been identified who differ in their disc wedging response upon extended asymmetrical compression. Annulus fibrosus (AF) tissue rem...

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Bibliographic Details
Published in:Journal of orthopaedic research 2021-09, Vol.39 (9), p.1955-1964
Main Authors: Tromp, Isabel N., Foolen, Jasper, Doeselaar, Marina, Zhang, Ying, Chan, Danny, Kruyt, Moyo C., Creemers, Laura B., Castelein, Rene M., Ito, Keita
Format: Article
Language:English
Subjects:
Animals
annulus fibrosus
Annulus Fibrosus - metabolism
collagen
Collagen - metabolism
intervertebral disc
Intervertebral Disc - metabolism
Intervertebral Disc Degeneration - metabolism
matrix remodeling
Mice
Mice, Inbred C57BL
scoliosis
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Summary:It has been suggested that curvature progression in adolescent idiopathic scoliosis occurs through irreversible changes in the intervertebral discs. Strains of mice have been identified who differ in their disc wedging response upon extended asymmetrical compression. Annulus fibrosus (AF) tissue remodeling could contribute to the faster disc wedging progression previously observed in these mice. Differences in collagen remodeling capacity of AF cells between these in‐bred mice strains were compared using an in vitro microtissue system. AF cells of 8–10‐week‐old LG/J (“fast‐healing”) and C57BL/6J (“normal healing”) mice were embedded in a microtissue platform and cultured for 48 h. Hereafter, tissues were partially released and cultured for another 96 h. Microtissue surface area and waistcoat contraction, collagen orientation, and collagen content were measured. After 96 h postrelease, microtissues with AF cells of LG/J mice showed more surface area contraction (p 
ISSN:0736-0266
1554-527X
DOI:10.1002/jor.24921