Antibacterial and Therapeutic Potentials of the Capsicum annuum Extract against Infected Wound in a Rat Model with Its Mechanisms of Antibacterial Action

• Published in: BioMed research international 2021, Vol.2021 (1), p.4303902
• Main Authors: Ekom, Steve Endeguele, Tamokou, Jean-De-Dieu, Kuete, Victor
• Format: Article
• Language: English
• Subjects: Adenosine Triphosphatases - metabolism; Animals; Anti-Bacterial Agents - pharmacology; Anti-Bacterial Agents - therapeutic use; Antibacterial activity; Antibacterial agents; Antibiotics; Antimicrobial agents; Bacteria; Bacteria - drug effects; Bacteria - enzymology; Biofilms; Biofilms - drug effects; Capsicum - chemistry; Capsicum annuum; Care and treatment; Cell Membrane Permeability - drug effects; Cell membranes; Disease Models, Animal; Dosage and administration; E coli; Electron Transport - drug effects; Eye - drug effects; Flavonoids; Flavonoids - analysis; Fruit - chemistry; Fruits; Gram-positive bacteria; Health aspects; Infectious diseases; L-Lactate Dehydrogenase - metabolism; Male; Materia medica, Vegetable; Methanol; Methanol - chemistry; Microbial Sensitivity Tests; Nucleic acids; Peppers; Permeability; Phenolic compounds; Phenols; Phenols - analysis; Plant extracts; Plant Extracts - pharmacology; Plant Extracts - therapeutic use; Prevention; Properties; Proteins; Proton Pumps - metabolism; Rats; Rats, Wistar; Risk factors; Skin - drug effects; Spectrophotometry; Staphylococcus aureus; Staphylococcus aureus infections; Sugar; Sugars - analysis; Sweet peppers; Tannins; Tannins - analysis; Wound healing; Wound Infection - drug therapy; Wound Infection - microbiology; Wounds and injuries
• ISSN: 2314-6133; 2314-6141; 2314-6141
• DOI: 10.1155/2021/4303902

The wound healing process is essential to reform the damaged tissue and prevent its invasion by pathogens. The present study aims at evaluating the antibacterial and therapeutic properties of the Capsicum annuum L. (Solanaceae) extract against infected wound in a rat model with its mechanisms of antibacterial action. The fruit extract was prepared by maceration in methanol. The broth microdilution method was used to investigate the antibacterial activity of the methanol extract of C. annuum fruits. The therapeutic effect of the extract gel was performed on an excision wound infected with Staphylococcus aureus using a rat model. The total phenol, flavonoid, and tannin contents as well as the antibacterial mechanisms of action of the extract were determined using spectrophotometric methods. The C. annuum fruit extract showed antibacterial properties which can be linked to its total phenolic, flavonoid, and tannin contents. The antibacterial activity is due to the inhibition of the biofilm formation, ATPases/H+ proton pump, and dehydrogenase activity as well as the alteration of the bacterial cell membrane through the leakage of nucleic acids, reducing sugars and proteins. The extract gel showed a significant (p