Calcium-Silicate-Incorporated Gellan-Chitosan Induced Osteogenic Differentiation in Mesenchymal Stromal Cells

Gellan-chitosan (GC) incorporated with CS: 0% (GC-0 CS), 10% (GC-10 CS), 20% (GC-20 CS) or 40% (GC-40 CS) / was prepared using freeze-drying method to investigate its physicochemical, biocompatible, and osteoinductive properties in human bone-marrow mesenchymal stromal cells (hBMSCs). The compositio...

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Bibliographic Details
Published in:Polymers 2021-09, Vol.13 (19), p.3211
Main Authors: Genasan, Krishnamurithy, Mehrali, Mohammad, Veerappan, Tarini, Talebian, Sepehr, Malliga Raman, Murali, Singh, Simmrat, Swamiappan, Sasikumar, Mehrali, Mehdi, Kamarul, Tunku, Balaji Raghavendran, Hanumantha Rao
Format: Article
Language:English
Subjects:
Alizarin
Biocompatibility
Biomedical materials
Bone marrow
Calcium silicates
Cell growth
Chitosan
Differentiation (biology)
Gene expression
Laboratories
Phosphatase
Photomicrographs
Polymers
Scanning electron microscopy
Tissue engineering
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Summary:Gellan-chitosan (GC) incorporated with CS: 0% (GC-0 CS), 10% (GC-10 CS), 20% (GC-20 CS) or 40% (GC-40 CS) / was prepared using freeze-drying method to investigate its physicochemical, biocompatible, and osteoinductive properties in human bone-marrow mesenchymal stromal cells (hBMSCs). The composition of different groups was reflected in physicochemical analyses performed using BET, FTIR, and XRD. The SEM micrographs revealed excellent hBMSCs attachment in GC-40 CS. The Alamar Blue assay indicated an increased proliferation and viability of seeded hBMSCs in all groups on day 21 as compared with day 0. The hBMSCs seeded in GC-40 CS indicated osteogenic differentiation based on an amplified alkaline-phosphatase release on day 7 and 14 as compared with day 0. These cells supported bone mineralization on GC-40 CS based on Alizarin-Red assay on day 21 as compared with day 7 and increased their osteogenic gene expression (RUNX2, ALP, BGLAP, BMP, and Osteonectin) on day 21. The GC-40 CS-seeded hBMSCs initiated their osteogenic differentiation on day 7 as compared with counterparts based on an increased expression of type-1 collagen and BMP2 in immunocytochemistry analysis. In conclusion, the incorporation of 40% ( / ) calcium silicate in gellan-chitosan showed osteoinduction potential in hBMSCs, making it a potential biomaterial to treat critical bone defects.
ISSN:2073-4360
2073-4360
DOI:10.3390/polym13193211