The Abundance and Organization of Salmonella Extracellular Polymeric Substances in Gallbladder-Mimicking Environments and In Vivo

Salmonella enterica serovar Typhi causes chronic infections by establishing biofilms on cholesterol gallstones. The production of extracellular polymeric substances (EPSs) is key to biofilm development, and biofilm architecture depends on which EPSs are made. The presence and spatial distribution of...

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Bibliographic Details
Published in:Infection and immunity 2021-10, Vol.89 (11), p.e0031021-e0031021
Main Authors: Hahn, Mark M, González, Juan F, Hitt, Regan, Tucker, Lauren, Gunn, John S
Format: Article
Language:English
Subjects:
Animals
Biofilms - growth & development
Cholesterol - metabolism
Conduct of Scientific Research
Extracellular Polymeric Substance Matrix - physiology
Female
Gallstones - microbiology
Host-Associated Microbial Communities
Humans
Mice
Salmonella typhi - physiology
Salmonella typhimurium - physiology
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Summary:Salmonella enterica serovar Typhi causes chronic infections by establishing biofilms on cholesterol gallstones. The production of extracellular polymeric substances (EPSs) is key to biofilm development, and biofilm architecture depends on which EPSs are made. The presence and spatial distribution of EPSs produced and were investigated in Salmonella enterica serovar Typhimurium and . Typhi biofilms by confocal microscopy. Comparisons between serovars and EPS-mutant bacteria were carried out by examining growth on cholesterol-coated surfaces, with human gallstones in ox or human bile, and in mice with gallstones. On cholesterol-coated surfaces, no major differences in EPS biomass were found between serovars. Cocultured biofilms containing wild-type (WT) and EPS-mutant bacteria demonstrated WT compensation for EPS mutations. Analysis of biofilm EPSs from gallbladder-mimicking conditions found that culture in human bile more consistently replicated the relative abundance and spatial organization of each EPS on gallstones from the chronic mouse model than culture in ox bile. . Typhimurium biofilms cultured on gallstones in ox bile exhibited colocalized pairings of curli fimbriae/lipopolysaccharide and O-antigen capsule/cellulose, while these associations were not present in . Typhi biofilms or in mouse gallstone biofilms. In general, the inclusion of human bile with gallstones replicated biofilm development on gallstones , demonstrating the strength of this model for studying biofilm parameters or EPS-directed therapeutic treatments.
ISSN:0019-9567
1098-5522
DOI:10.1128/IAI.00310-21