APOL1 genotype-associated morphologic changes among patients with focal segmental glomerulosclerosis

• Published in: Pediatric nephrology (Berlin, West) West), 2021-09, Vol.36 (9), p.2747-2757
• Main Authors: Zee, Jarcy, McNulty, Michelle T., Hodgin, Jeffrey B., Zhdanova, Olga, Hingorani, Sangeeta, Jefferson, Jonathan Ashley, Gibson, Keisha L., Trachtman, Howard, Fornoni, Alessia, Dell, Katherine M., Reich, Heather N., Bagnasco, Serena, Greenbaum, Larry A., Lafayette, Richard A., Gipson, Debbie S., Brown, Elizabeth, Kretzler, Matthias, Appel, Gerald, Sambandam, Kamalanathan K., Tuttle, Katherine R., Chen, Dhruti, Atkinson, Meredith A., Hogan, Marie C., Kaskel, Frederick J., Meyers, Kevin E., O’Toole, John, Srivastava, Tarak, Sethna, Christine B., Hladunewich, Michelle A., Lin, JJ, Nast, Cynthia C., Derebail, Vimal K., Patel, Jiten, Vento, Suzanne, Holzman, Lawrence B., Athavale, Ambarish M., Adler, Sharon G., Lemley, Kevin V., Lieske, John C., Hogan, Jonathan J., Gadegbeku, Crystal A., Fervenza, Fernando C., Wang, Chia-Shi, Matar, Raed Bou, Singer, Pamela, Kopp, Jeffrey B., Barisoni, Laura, Sampson, Matthew G.
• Format: Article
• Language: English
• Subjects: Alleles; Apolipoprotein L1 - genetics; Apolipoproteins; Biopsy; Care and treatment; Cytoskeleton; Demographic aspects; Gene expression; Genotype; Genotypes; Glomerulosclerosis, Focal Segmental - genetics; Humans; Kidneys; Laboratory animals; Learning algorithms; Medicine; Medicine & Public Health; Microcysts; Molecular modelling; Nephrology; Nephrotic syndrome; Nephrotic Syndrome - genetics; Original Article; Patients; Pediatrics; Population genetics; Risk factors; Urology
• ISSN: 0931-041X; 1432-198X
• DOI: 10.1007/s00467-021-04990-4

Background The G1 and G2 alleles of apolipoprotein L1 ( APOL1 ) are common in the Black population and associated with increased risk of focal segmental glomerulosclerosis (FSGS). The molecular mechanisms linking APOL1 risk variants with FSGS are not clearly understood, and APOL1’s natural absence in laboratory animals makes studying its pathobiology challenging. Methods In a cohort of 90 Black patients with either FSGS or minimal change disease (MCD) enrolled in the Nephrotic Syndrome Study Network (58% pediatric onset), we used kidney biopsy traits as an intermediate outcome to help illuminate tissue-based consequences of APOL1 risk variants and expression. We tested associations between APOL1 risk alleles or glomerular APOL1 mRNA expression and 83 light- or electron-microscopy traits measuring structural and cellular kidney changes. Results Under both recessive and dominant models in the FSGS patient subgroup (61%), APOL1 risk variants were significantly correlated (defined as FDR