Renal Denervation Mitigated Fecal Microbiota Aberrations in Rats with Chronic Heart Failure

• Published in: Evidence-based complementary and alternative medicine 2021, Vol.2021, p.1697004-17
• Main Authors: Guo, Zhiqin, Chen, Yufeng, Chen, Shuoxian, Liu, Chao, Li, Shaonan, Chen, Pingan
• Format: Article
• Language: English
• Subjects: Abdomen; Ablation; Alistipes; Aorta; Bacteria; Bacteroides; Cardiac function; Chronic illnesses; Clostridium; Congestive heart failure; Coronary vessels; Denervation; Digestive system; Discriminant analysis; Fecal microflora; Gastrointestinal tract; Genetic testing; Heart failure; Intestinal microflora; Kidneys; Laboratory animals; Lactobacillus; Microbiomes; Microbiota; Nogo protein; Plasma; Reinnervation; Renal failure; Software; Sympathetic nerves
• ISSN: 1741-427X; 1741-4288
• DOI: 10.1155/2021/1697004

Background. Changes in the composition and diversity of gut microbiota, which can be altered by autonomic nerve activity, contribute to the development of heart failure (HF). Renal denervation (RDN) can improve cardiac function by reducing sympathetic nerve activity. However, whether the beneficial role of RDN on HF is related to gut microbiota is unknown. Methods. Thirty rats were assigned to a control, HF (with induced transverse aortic constriction (TAC)), RDN (with RDN induced 10 weeks after TAC), Nog (HF rats with Nogo-P4-administered 8 weeks after RDN), and NEP (HF rats with NEP1-40-administered 8 weeks after RDN) group. Then, 16SrRNA amplicon sequencing and analyses of fecal samples were performed. Results. Beta diversity analyses revealed that compared to the HF group, the RDN, Nog, and NEP groups clustered closer to the control group. The Firmicutes/Bacteroidetes ratio was reduced in the HF group (1.59) compared with the control group (3.21) and was significantly decreased compared to the Nog (7.19), RDN (6.20), and NEP (4.42) groups. At the genus level, the HF group showed decreased abundances of Lactobacillus and Alistipes and increased abundances of Bacteroides and Clostridium compared with the control group. The abundances of Lactobacillus and Alistipes were increased, and those of Bacteroides and Clostridium were decreased in the RDN, Nog, and NEP groups compared to the HF group. However, no differences were observed between the three groups that underwent RDN. The microbial function showed the same tendency. Conclusions. RDN reversed the abnormal changes in the gut microbiome in HF rats. Inhibition of reinnervation after RDN did not affect intestinal bacteria.