Assessment of Disinfection Potential of Q-Switch Nd: YAG Laser on Contaminated Titanium Implant Surfaces

• Published in: Materials 2021-10, Vol.14 (20), p.6078
• Main Authors: Namour, Melanie, El Mobadder, Marwan, Mulongo, Baudouin, Fagnart, Olivier, Harb, Assaf, Peremans, André, Verspecht, Tim, Teughels, Wim, Nammour, Samir, Rompen, Eric
• Format: Article
• Language: English
• Subjects: Actinomyces; Bacteria; Bacterial infections; Biofilms; Carbon dioxide; Colony forming units; Condensed Matter Physics; Contamination; Debridement; dental implant; Dental implants; Dentisterie & médecine buccale; Dentistry & oral medicine; Disinfection; Flux density; General Materials Science; Human health sciences; implant; Laboratories; laser; Laser beams; Lasers; Materials Science (all); Micrometers; Nd:YAG laser; Neodymium lasers; peri-implantitis; Periodontics; Periodontium; periodontology; Pulse duration; Q-switches; Sciences de la santé humaine; Semiconductor lasers; Statistical methods; Titania surfaces; Titanium; Titanium disks; Titanium implant surface; YAG lasers
• ISSN: 1996-1944; 1996-1944
• DOI: 10.3390/ma14206078

Peri-implantitis (PI) is a relatively frequent pathology that compromises the overall survival of the dental implant. Adjunctive approaches for the conventional mechanical debridement are being suggested to optimize the treatment of PI. The goal of the study was the assessment of the disinfection potential of the Q-Switch Nd: YAG laser on contaminated titanium implant surfaces. A total of 72 sterile titanium discs were used and divided into three groups: 24 contaminated titanium discs treated with the laser (study Group L), 24 contaminated titanium discs with no treatment (control 1—Group C), and 24 sterile titanium discs with no treatment (control 2—Group S). Multi-species biofilm was used: Porphyromonas gingivalis, Fusobacterium nucleatum, Aggregatibacter actinomycetemcomitans, Streptococcus mutans, Streptococcus sobrinus, and Prevotella intermedia. Commensal bacteria were included also: Actinomyces naeslundii, Actinomyces viscosus, Streptococcus cristatus, Streptococcus gordonii, Streptococcus mitis, Streptococcus oralis, Streptococcus sanguinis, Streptococcus parasanguinis, and Veillonella parvula. Parameters delivered per pulse on the targeted surfaces of the titanium discs were an energy density of 0.597 J/cm2 each pulse, a pulse power of 270 mW, a laser beam spot of 2.4 mm in diameter, and a rate of repetition of 10 Hertz (Hz) for a pulse duration of 6 nanoseconds (ns). The mode was no contact, and a distance of 500 micrometers was used with a total time of irradiation equal to 2 s (s). The collection of microbiological samples was made for all groups; colony-forming units (CFU) were identified by two different practitioners, and the average of their examinations was considered for each sample. The average of the TBC (CFU/mL) was calculated for each group. Values were 0.000 CFU/mL, 4767 CFU/mL, and 0.000 CFU/mL for Group L, Group C, and Group S, respectively. Therefore, the suggested treatment protocol was able to provoke a total disinfection of the contaminated titanium surfaces. A statistical difference was only found between Group L vs. Group C and between Group S vs. Group C. The difference was not significant between Group S and Group L. In conclusion, the present study confirmed that the Q-Switch Nd: YAG laser under our specific conditions can provide a total disinfection of the contaminated titanium surfaces.