In vitro mature embryo culture protocol of einkorn (Triticum monococcum ssp. monococcum) and bread (Triticum aestivum L.) wheat under boron stress

Mature embryos of einkorn ( Triticum monococcum ssp. monococcum ) and bread ( Triticum aestivum L.) wheat were used for callus induction on media containing four different doses (0, 1, 2 and 4 mg L −1 ) of 2,4-D and dicamba supplemented with five different boron concentrations (0, 6.2, 12.4, 24.8, a...

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Bibliographic Details
Published in:Plant cell, tissue and organ culture tissue and organ culture, 2022-02, Vol.148 (2), p.293-304
Main Authors: Ağıl, Ferdi, Örgeç, Mehmet, Karakaş, Fatma Pehlivan, Verma, Sandeep Kumar, Zencirci, Nusret
Format: Article
Language:English
Subjects:
2,4-D
Biomedical and Life Sciences
Boron
Bread
Callus
Culture media
Embryos
Life Sciences
Original
Original Article
Plant breeding
Plant Genetics and Genomics
Plant Pathology
Plant Physiology
Plant Sciences
Regeneration
Triticum aestivum
Triticum monococcum
Wheat
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Summary:Mature embryos of einkorn ( Triticum monococcum ssp. monococcum ) and bread ( Triticum aestivum L.) wheat were used for callus induction on media containing four different doses (0, 1, 2 and 4 mg L −1 ) of 2,4-D and dicamba supplemented with five different boron concentrations (0, 6.2, 12.4, 24.8, and 37.2 mg L −1 ). The obtained callus was transferred to culture media with three (0, 0.5, and 2 mg L −1 ) different BAP doses with five boron concentrations for further regeneration. The maximum callus weight in einkorn wheat was in culture media with 1 mg L −1 dicamba and 6.2 mg L −1 (3.71 ± 0.13 g). Bread wheat had the maximum callus weight on culture media with 4 mg L −1 dicamba and 12.4 mg L −1 (3.46 ± 0.40 g). The highest plantlet numbers were in only 2 mg L −1 BAP (2.92 ± 0.88) for einkorn wheat and 0.5 mg L −1 BAP supplemented with 6.2 mg L −1 boron (3.71 ± 1.12) for bread wheat. This indirect regeneration protocol using mature embryos of einkorn and bread wheat under boron stresses expected to be useful for future wheat breeding studies. Key message The embryo tissues of different types of wheat have provided to develop under in vitro conditions by applying various boron concentrations. 2,4-D and dicamba, which are different auxin types, played an effective role in the callus formation.
ISSN:0167-6857
1573-5044
DOI:10.1007/s11240-021-02186-0