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In vitro mature embryo culture protocol of einkorn (Triticum monococcum ssp. monococcum) and bread (Triticum aestivum L.) wheat under boron stress
Mature embryos of einkorn ( Triticum monococcum ssp. monococcum ) and bread ( Triticum aestivum L.) wheat were used for callus induction on media containing four different doses (0, 1, 2 and 4 mg L −1 ) of 2,4-D and dicamba supplemented with five different boron concentrations (0, 6.2, 12.4, 24.8, a...
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| Published in: | Plant cell, tissue and organ culture tissue and organ culture, 2022-02, Vol.148 (2), p.293-304 |
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| container_issue | 2 |
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| container_title | Plant cell, tissue and organ culture |
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| creator | Ağıl, Ferdi Örgeç, Mehmet Karakaş, Fatma Pehlivan Verma, Sandeep Kumar Zencirci, Nusret |
| description | Mature embryos of einkorn (
Triticum monococcum
ssp.
monococcum
) and bread (
Triticum aestivum
L.) wheat were used for callus induction on media containing four different doses (0, 1, 2 and 4 mg L
−1
) of 2,4-D and dicamba supplemented with five different boron concentrations (0, 6.2, 12.4, 24.8, and 37.2 mg L
−1
). The obtained callus was transferred to culture media with three (0, 0.5, and 2 mg L
−1
) different BAP doses with five boron concentrations for further regeneration. The maximum callus weight in einkorn wheat was in culture media with 1 mg L
−1
dicamba and 6.2 mg L
−1
(3.71 ± 0.13 g). Bread wheat had the maximum callus weight on culture media with 4 mg L
−1
dicamba and 12.4 mg L
−1
(3.46 ± 0.40 g). The highest plantlet numbers were in only 2 mg L
−1
BAP (2.92 ± 0.88) for einkorn wheat and 0.5 mg L
−1
BAP supplemented with 6.2 mg L
−1
boron (3.71 ± 1.12) for bread wheat. This indirect regeneration protocol using mature embryos of einkorn and bread wheat under boron stresses expected to be useful for future wheat breeding studies.
Key message
The embryo tissues of different types of wheat have provided to develop under in vitro conditions by applying various boron concentrations. 2,4-D and dicamba, which are different auxin types, played an effective role in the callus formation. |
| doi_str_mv | 10.1007/s11240-021-02186-0 |
| format | article |
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Triticum monococcum
ssp.
monococcum
) and bread (
Triticum aestivum
L.) wheat were used for callus induction on media containing four different doses (0, 1, 2 and 4 mg L
−1
) of 2,4-D and dicamba supplemented with five different boron concentrations (0, 6.2, 12.4, 24.8, and 37.2 mg L
−1
). The obtained callus was transferred to culture media with three (0, 0.5, and 2 mg L
−1
) different BAP doses with five boron concentrations for further regeneration. The maximum callus weight in einkorn wheat was in culture media with 1 mg L
−1
dicamba and 6.2 mg L
−1
(3.71 ± 0.13 g). Bread wheat had the maximum callus weight on culture media with 4 mg L
−1
dicamba and 12.4 mg L
−1
(3.46 ± 0.40 g). The highest plantlet numbers were in only 2 mg L
−1
BAP (2.92 ± 0.88) for einkorn wheat and 0.5 mg L
−1
BAP supplemented with 6.2 mg L
−1
boron (3.71 ± 1.12) for bread wheat. This indirect regeneration protocol using mature embryos of einkorn and bread wheat under boron stresses expected to be useful for future wheat breeding studies.
Key message
The embryo tissues of different types of wheat have provided to develop under in vitro conditions by applying various boron concentrations. 2,4-D and dicamba, which are different auxin types, played an effective role in the callus formation.</description><identifier>ISSN: 0167-6857</identifier><identifier>EISSN: 1573-5044</identifier><identifier>DOI: 10.1007/s11240-021-02186-0</identifier><identifier>PMID: 34720279</identifier><language>eng</language><publisher>Dordrecht: Springer Netherlands</publisher><subject>2,4-D ; Biomedical and Life Sciences ; Boron ; Bread ; Callus ; Culture media ; Embryos ; Life Sciences ; Original ; Original Article ; Plant breeding ; Plant Genetics and Genomics ; Plant Pathology ; Plant Physiology ; Plant Sciences ; Regeneration ; Triticum aestivum ; Triticum monococcum ; Wheat</subject><ispartof>Plant cell, tissue and organ culture, 2022-02, Vol.148 (2), p.293-304</ispartof><rights>The Author(s), under exclusive licence to Springer Nature B.V. 2021</rights><rights>The Author(s), under exclusive licence to Springer Nature B.V. 2021.</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c474t-724b720d6be9152069f4651d3e887878041a3fe435e50f264e5e084661056453</citedby><cites>FETCH-LOGICAL-c474t-724b720d6be9152069f4651d3e887878041a3fe435e50f264e5e084661056453</cites><orcidid>0000-0003-3460-7575</orcidid></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>230,314,780,784,885,27923,27924</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/34720279$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Ağıl, Ferdi</creatorcontrib><creatorcontrib>Örgeç, Mehmet</creatorcontrib><creatorcontrib>Karakaş, Fatma Pehlivan</creatorcontrib><creatorcontrib>Verma, Sandeep Kumar</creatorcontrib><creatorcontrib>Zencirci, Nusret</creatorcontrib><title>In vitro mature embryo culture protocol of einkorn (Triticum monococcum ssp. monococcum) and bread (Triticum aestivum L.) wheat under boron stress</title><title>Plant cell, tissue and organ culture</title><addtitle>Plant Cell Tiss Organ Cult</addtitle><addtitle>Plant Cell Tissue Organ Cult</addtitle><description>Mature embryos of einkorn (
Triticum monococcum
ssp.
monococcum
) and bread (
Triticum aestivum
L.) wheat were used for callus induction on media containing four different doses (0, 1, 2 and 4 mg L
−1
) of 2,4-D and dicamba supplemented with five different boron concentrations (0, 6.2, 12.4, 24.8, and 37.2 mg L
−1
). The obtained callus was transferred to culture media with three (0, 0.5, and 2 mg L
−1
) different BAP doses with five boron concentrations for further regeneration. The maximum callus weight in einkorn wheat was in culture media with 1 mg L
−1
dicamba and 6.2 mg L
−1
(3.71 ± 0.13 g). Bread wheat had the maximum callus weight on culture media with 4 mg L
−1
dicamba and 12.4 mg L
−1
(3.46 ± 0.40 g). The highest plantlet numbers were in only 2 mg L
−1
BAP (2.92 ± 0.88) for einkorn wheat and 0.5 mg L
−1
BAP supplemented with 6.2 mg L
−1
boron (3.71 ± 1.12) for bread wheat. This indirect regeneration protocol using mature embryos of einkorn and bread wheat under boron stresses expected to be useful for future wheat breeding studies.
Key message
The embryo tissues of different types of wheat have provided to develop under in vitro conditions by applying various boron concentrations. 2,4-D and dicamba, which are different auxin types, played an effective role in the callus formation.</description><subject>2,4-D</subject><subject>Biomedical and Life Sciences</subject><subject>Boron</subject><subject>Bread</subject><subject>Callus</subject><subject>Culture media</subject><subject>Embryos</subject><subject>Life Sciences</subject><subject>Original</subject><subject>Original Article</subject><subject>Plant breeding</subject><subject>Plant Genetics and Genomics</subject><subject>Plant Pathology</subject><subject>Plant Physiology</subject><subject>Plant Sciences</subject><subject>Regeneration</subject><subject>Triticum aestivum</subject><subject>Triticum monococcum</subject><subject>Wheat</subject><issn>0167-6857</issn><issn>1573-5044</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2022</creationdate><recordtype>article</recordtype><recordid>eNp9UU1v1DAUtBCIbgt_gAOyxKU9ZPv8meSChCoolVbqZe-Wk7y0KYm92M6i_o3-4nq7pSwckGX5Pc28scdDyAcGSwZQnkfGuIQCONvtShfwiiyYKkWhQMrXZAFMl4WuVHlEjmO8AwAtJHtLjoQsOfCyXpCHK0e3QwqeTjbNASlOTbj3tJ3Hp3YTfPKtH6nvKQ7uhw-Onq7DkIZ2nujkXQbbXRnjZnnQn1HrOtoEtN0B32JMwzYXq-UZ_XWLNtHZdRho44N3NKaAMb4jb3o7Rnz_fJ6Q9bev64vvxer68uriy6poZSlTUXLZZBedbrBmioOue6kV6wRWVZkXSGZFj1IoVNBzLVEhVFJrBkpLJU7I573sZm4m7Fp0KdjRbMIw2XBvvB3M34gbbs2N35pKyfyLdRY4fRYI_uecnZlpiC2Oo3Xo52i4qhnngtdVpn76h3rn5-CyO8M1h0pUku9YfM9qg48xYP_yGAZml7jZJ25y2uYpcQN56OOhjZeR3xFngtgTYobcDYY_d_9H9hEEHbec</recordid><startdate>20220201</startdate><enddate>20220201</enddate><creator>Ağıl, Ferdi</creator><creator>Örgeç, Mehmet</creator><creator>Karakaş, Fatma Pehlivan</creator><creator>Verma, Sandeep Kumar</creator><creator>Zencirci, Nusret</creator><general>Springer Netherlands</general><general>Springer Nature B.V</general><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>3V.</scope><scope>7X2</scope><scope>8FE</scope><scope>8FH</scope><scope>8FK</scope><scope>AEUYN</scope><scope>AFKRA</scope><scope>ATCPS</scope><scope>AZQEC</scope><scope>BBNVY</scope><scope>BENPR</scope><scope>BHPHI</scope><scope>CCPQU</scope><scope>DWQXO</scope><scope>GNUQQ</scope><scope>HCIFZ</scope><scope>LK8</scope><scope>M0K</scope><scope>M7P</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>7X8</scope><scope>5PM</scope><orcidid>https://orcid.org/0000-0003-3460-7575</orcidid></search><sort><creationdate>20220201</creationdate><title>In vitro mature embryo culture protocol of einkorn (Triticum monococcum ssp. monococcum) and bread (Triticum aestivum L.) wheat under boron stress</title><author>Ağıl, Ferdi ; Örgeç, Mehmet ; Karakaş, Fatma Pehlivan ; Verma, Sandeep Kumar ; Zencirci, Nusret</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c474t-724b720d6be9152069f4651d3e887878041a3fe435e50f264e5e084661056453</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2022</creationdate><topic>2,4-D</topic><topic>Biomedical and Life Sciences</topic><topic>Boron</topic><topic>Bread</topic><topic>Callus</topic><topic>Culture media</topic><topic>Embryos</topic><topic>Life Sciences</topic><topic>Original</topic><topic>Original Article</topic><topic>Plant breeding</topic><topic>Plant Genetics and Genomics</topic><topic>Plant Pathology</topic><topic>Plant Physiology</topic><topic>Plant Sciences</topic><topic>Regeneration</topic><topic>Triticum aestivum</topic><topic>Triticum monococcum</topic><topic>Wheat</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Ağıl, Ferdi</creatorcontrib><creatorcontrib>Örgeç, Mehmet</creatorcontrib><creatorcontrib>Karakaş, Fatma Pehlivan</creatorcontrib><creatorcontrib>Verma, Sandeep Kumar</creatorcontrib><creatorcontrib>Zencirci, Nusret</creatorcontrib><collection>PubMed</collection><collection>CrossRef</collection><collection>ProQuest Central (Corporate)</collection><collection>Agricultural Science Collection</collection><collection>ProQuest SciTech Collection</collection><collection>ProQuest Natural Science Collection</collection><collection>ProQuest Central (Alumni) (purchase pre-March 2016)</collection><collection>ProQuest One Sustainability</collection><collection>ProQuest Central</collection><collection>Agricultural & Environmental Science Collection</collection><collection>ProQuest Central Essentials</collection><collection>Biological Science Collection</collection><collection>ProQuest Central</collection><collection>Natural Science Collection</collection><collection>ProQuest One Community College</collection><collection>ProQuest Central Korea</collection><collection>ProQuest Central Student</collection><collection>SciTech Premium Collection</collection><collection>ProQuest Biological Science Collection</collection><collection>Agriculture Science Database</collection><collection>Biological Science Database</collection><collection>ProQuest One Academic Eastern Edition (DO NOT USE)</collection><collection>ProQuest One Academic</collection><collection>ProQuest One Academic UKI Edition</collection><collection>MEDLINE - Academic</collection><collection>PubMed Central (Full Participant titles)</collection><jtitle>Plant cell, tissue and organ culture</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Ağıl, Ferdi</au><au>Örgeç, Mehmet</au><au>Karakaş, Fatma Pehlivan</au><au>Verma, Sandeep Kumar</au><au>Zencirci, Nusret</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>In vitro mature embryo culture protocol of einkorn (Triticum monococcum ssp. monococcum) and bread (Triticum aestivum L.) wheat under boron stress</atitle><jtitle>Plant cell, tissue and organ culture</jtitle><stitle>Plant Cell Tiss Organ Cult</stitle><addtitle>Plant Cell Tissue Organ Cult</addtitle><date>2022-02-01</date><risdate>2022</risdate><volume>148</volume><issue>2</issue><spage>293</spage><epage>304</epage><pages>293-304</pages><issn>0167-6857</issn><eissn>1573-5044</eissn><abstract>Mature embryos of einkorn (
Triticum monococcum
ssp.
monococcum
) and bread (
Triticum aestivum
L.) wheat were used for callus induction on media containing four different doses (0, 1, 2 and 4 mg L
−1
) of 2,4-D and dicamba supplemented with five different boron concentrations (0, 6.2, 12.4, 24.8, and 37.2 mg L
−1
). The obtained callus was transferred to culture media with three (0, 0.5, and 2 mg L
−1
) different BAP doses with five boron concentrations for further regeneration. The maximum callus weight in einkorn wheat was in culture media with 1 mg L
−1
dicamba and 6.2 mg L
−1
(3.71 ± 0.13 g). Bread wheat had the maximum callus weight on culture media with 4 mg L
−1
dicamba and 12.4 mg L
−1
(3.46 ± 0.40 g). The highest plantlet numbers were in only 2 mg L
−1
BAP (2.92 ± 0.88) for einkorn wheat and 0.5 mg L
−1
BAP supplemented with 6.2 mg L
−1
boron (3.71 ± 1.12) for bread wheat. This indirect regeneration protocol using mature embryos of einkorn and bread wheat under boron stresses expected to be useful for future wheat breeding studies.
Key message
The embryo tissues of different types of wheat have provided to develop under in vitro conditions by applying various boron concentrations. 2,4-D and dicamba, which are different auxin types, played an effective role in the callus formation.</abstract><cop>Dordrecht</cop><pub>Springer Netherlands</pub><pmid>34720279</pmid><doi>10.1007/s11240-021-02186-0</doi><tpages>12</tpages><orcidid>https://orcid.org/0000-0003-3460-7575</orcidid><oa>free_for_read</oa></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 0167-6857 |
| ispartof | Plant cell, tissue and organ culture, 2022-02, Vol.148 (2), p.293-304 |
| issn | 0167-6857 1573-5044 |
| language | eng |
| recordid | cdi_pubmedcentral_primary_oai_pubmedcentral_nih_gov_8543419 |
| source | Springer Nature |
| subjects | 2,4-D Biomedical and Life Sciences Boron Bread Callus Culture media Embryos Life Sciences Original Original Article Plant breeding Plant Genetics and Genomics Plant Pathology Plant Physiology Plant Sciences Regeneration Triticum aestivum Triticum monococcum Wheat |
| title | In vitro mature embryo culture protocol of einkorn (Triticum monococcum ssp. monococcum) and bread (Triticum aestivum L.) wheat under boron stress |
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