Study on the Effects of Chinese Materia Medica Processing on the Hypoglycemic Activity and Chemical Composition of Anemarrhenae Rhizoma

Purpose. To compare the hypoglycemic effects of different extracts of Anemarrhenae Rhizoma (AR) before and after being stir-baked with salt water on the diabetic mice and to detect the contents of 8 components in the corresponding active parts simultaneously using the UPLC-MS method, in order to scr...

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Bibliographic Details
Published in:Evidence-based complementary and alternative medicine 2021, Vol.2021, p.6211609-11
Main Authors: Yu, Ying-Qi, Yan, Li, Wang, Xiao-Ting, Li, Li, Zheng, Wei, Gao, Hui
Format: Article
Language:English
Subjects:
Acetonitrile
Animal models
Biotechnology
Chemical composition
Chinese medicine
Chromatography
Cultural heritage
Data acquisition
Diabetes
Diabetes mellitus
Enzymes
Ethanol
Evidence-based medicine
Fasting
Formic acid
Glucose
Glucose tolerance
Hemoglobin
Hypoglycemia
Insulin
Insulin resistance
Ionization
Laboratory animals
Malondialdehyde
Medical research
Nitric oxide
Pharmacodynamics
Reagents
Saline water
Superoxide dismutase
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Summary:Purpose. To compare the hypoglycemic effects of different extracts of Anemarrhenae Rhizoma (AR) before and after being stir-baked with salt water on the diabetic mice and to detect the contents of 8 components in the corresponding active parts simultaneously using the UPLC-MS method, in order to screen the better extracts for diabetes and to clear the material basis for enhancing hypoglycemic activity of Anemarrhenae Rhizoma stir-baked with salt water (SAR). Methods. Taking spontaneous type II diabetic db/db mice as models and fasting blood glucose (FBG), oral glucose tolerance test (OGTT), glycated hemoglobin or glycosylated hemoglobin (HbAlc), serum resistin (RESISTEIN), fasting insulin (FINS), superoxide dismutase (SOD), malondialdehyde (MDA), and nitric oxide (NO) as indicators, the hypoglycemic effects of different active parts of Anemarrhenae Rhizoma were evaluated. The chromatographic separation was performed on a Waters BEH C18 (2.1 mm × 50 mm, 1.7 μm) column using acetonitrile (B) and 0.1% formic acid in water (A) as mobile phases, and the flow rate was 0.3 ml/min. The column temperature was set as 28°C, and the injection volume was 10 μL. A mass spectrometer was connected to the UPLC system via an electrospray ionization (ESI) interface. Full-scan data acquisition was performed in the negative ion mode. Result. In the study of pharmacodynamics, the hypoglycemic effect of Anemarrhenae Rhizoma stir-baked with salt water is better than that of Anemarrhenae Rhizoma and the hypoglycemic effect of ethanol extract of Anemarrhenae Rhizoma is more remarkable than that of the decoction. The measured components all have a good linear relationship within their respective linear ranges (r ≥ 0.9990); the average recovery rates are 98.86%–100.69%, RSD
ISSN:1741-427X
1741-4288
DOI:10.1155/2021/6211609