Hypothermic preservation of rat hearts using antifreeze glycoprotein

Antifreeze proteins are an effective additive for low-temperature preservation of solid organs. Here, we compared static hypothermic preservation with and without antifreeze glycoprotein (AFGP), followed by nonfreezing cryopreservation of rat hearts. The heart was surgically extracted and immersed i...

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Bibliographic Details
Published in:Physiological research 2021-03, Vol.69 (6), p.1029-1038
Main Authors: Takago, Shintaro, Matsumoto, Isao, Kato, Hiroki, Saito, Naoki, Ueda, Hideyasu, Iino, Kenji, Kimura, Keiichi, Takemura, Hirofumi
Format: Article
Language:English
Subjects:
Adenosine triphosphate
Antifreeze proteins
ATP
Cardiac function
Catheters
Coronary artery
Coronary vessels
Cryopreservation
Experiments
Glycoproteins
Heart rate
Laboratory animals
Low temperature
Metabolism
Muscle contraction
Ostomy
Potassium
Veins & arteries
Water content
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Summary:Antifreeze proteins are an effective additive for low-temperature preservation of solid organs. Here, we compared static hypothermic preservation with and without antifreeze glycoprotein (AFGP), followed by nonfreezing cryopreservation of rat hearts. The heart was surgically extracted and immersed in one of the cardioplegia solutions after cardiac arrest. Control rat hearts (n=6) were immersed in University of Wisconsin (UW) solution whereas AFGP-treated hearts (AFGP group) (n=6) were immersed in UW solution containing 500 μg/ml AFGP. After static hypothermic preservation, a Langendorff apparatus was used to reperfuse the coronary arteries with oxygenated Krebs-Henseleit solution. After 30, 60, 90, and 120 min, the heart rate (HR), coronary flow (CF), cardiac contractile force (max dP/dt), and cardiac diastolic force (min dP/dt) were measured. Tissue water content (TWC) and tissue adenosine triphosphate (ATP) levels in the reperfused preserved hearts were also assessed. All the parameters were compared between the control and AFGP groups. Compared with the control group, the AFGP group had significantly (p
ISSN:0862-8408
1802-9973
DOI:10.33549/physiolres.934473