effects of peptide-precipitating lignosulphonic acids on the proteolytic activity of pepsin in vitro and on the response of pigs to an ulcer inducing diet

The inhibitory effect of peptide-precipitating lignosulphonic acids on the proteolytic activity of pepsin in vitro has been studied. When an unfractionated lignosulphonic acid preparation was used, total inhibition of the activity of 5 µ g crystalline pepsin was obtained by addition of about 15 mg o...

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Bibliographic Details
Published in:Acta veterinaria scandinavica 1973-01, Vol.14 (1), p.44-56
Main Authors: Naess, B, Westbye, O, Hildrum, K.I, Nafstad, I
Format: Article
Language:English
Subjects:
animal diseases
Animal Feed
animal health
Animals
Arginine - pharmacology
Chemical Precipitation
Chromatography, Gel
Diet
Female
Hemoglobins - metabolism
Hydrogen-Ion Concentration
In Vitro Techniques
Lethal Dose 50
Lignin - pharmacology
Lignin - toxicity
Male
noninfectious diseases
Pepsin A - antagonists & inhibitors
Peptic Ulcer - chemically induced
Peptic Ulcer - pathology
Peptones - metabolism
Rats
Sulfonic Acids - pharmacology
Sulfonic Acids - toxicity
Swine
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Summary:The inhibitory effect of peptide-precipitating lignosulphonic acids on the proteolytic activity of pepsin in vitro has been studied. When an unfractionated lignosulphonic acid preparation was used, total inhibition of the activity of 5 µ g crystalline pepsin was obtained by addition of about 15 mg of lignosulphonic acids. The greatest inhibitory effect was seen with the high-molecular weight lignosulphonic acids, but an effect was also seen with middle-and low-molecular weight lignosulphonic acids. A reduction in the inhibitory effect was seen when the experiments were performed in the presence of arginine. The inhibition mechanism is discussed in relation to the peptide-precipitating ability of the lignosulphonic acids. No obvious protection against the appearance of stomach ulcers in four pigs receiving an ulcerogenic diet was seen.
ISSN:0044-605X
1751-0147
1751-0147
DOI:10.1186/BF03547409