Global DNA methylation in rats´ liver is not affected by hypercholesterolemic diet

Increased plasma cholesterol levels are listed between the major atherosclerosis risk factors. The final plasma cholesterol levels result from the interplay between the genetic and environmental (diet, physical activity) factors. Little is known, how dietary factors influence epigenetics. We have an...

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Bibliographic Details
Published in:Physiological research 2020-04, Vol.69 (2), p.347-352
Main Authors: Jurcikova-Novotna, L., Mrazova, L., Micova, K., Friedecky, D., Hubacek, J.A., Poledne, R.
Format: Article
Language:English
Subjects:
Animal models
Arteriosclerosis
Atherosclerosis
Cholesterol
Chromatography
Deoxyribonucleic acid
Diet
DNA
DNA methylation
Epigenetics
Feeds
Gene expression
Genetic testing
Genomes
High cholesterol diet
High fat diet
Liquid chromatography
Liver
Mass spectrometry
Mass spectroscopy
Physical activity
Risk factors
Rodents
Scientific imaging
Short Communication
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Summary:Increased plasma cholesterol levels are listed between the major atherosclerosis risk factors. The final plasma cholesterol levels result from the interplay between the genetic and environmental (diet, physical activity) factors. Little is known, how dietary factors influence epigenetics. We have analyzed, if an over-generation feeding of rat with cholesterol influences total liver-DNA methylation, and if total liver-DNA methylation differ between the different rat strains (Prague hereditary hypercholesterolemic rats, Prague hereditary hypertriglyceridemic rats and Wistar Kyoto rats). The animals were feed with high fat (additional 5 % over normal capacity) high cholesterol (2 %) diet for 14 days. DNA methylation in the liver tissue in different generations was analyzed using the liquid chromatography coupled with tandem mass spectrometry. We have not observed any significant changes in total liver-DNA methylation over the 9 generations of animals feed by fat/cholesterol enriched diet. Additionally, there were no differences in DNA methylation between different rat strains. In animal model, the dietary changes (hypercholesterolemic diet) not significantly influence the total DNA methylation status within the liver.
ISSN:0862-8408
1802-9973
DOI:10.33549/physiolres.934313