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In vivo 1H MR spectroscopy with J‐refocusing

Purpose The goal of this study was to propose a novel localized proton MR spectroscopy (MRS) sequence that reduces signal loss due to J‐modulation in the rat brain in vivo. Methods Sprague‐Dawley rats were studied at 9.4 T. A semi‐LASER sequence with evenly distributed echo‐time (TE) was used, and a...

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Bibliographic Details
Published in:Magnetic resonance in medicine 2021-12, Vol.86 (6), p.2957-2965
Main Authors: Deelchand, Dinesh K., Walls, Jamie D., Marjańska, Małgorzata
Format: Article
Language:English
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Summary:Purpose The goal of this study was to propose a novel localized proton MR spectroscopy (MRS) sequence that reduces signal loss due to J‐modulation in the rat brain in vivo. Methods Sprague‐Dawley rats were studied at 9.4 T. A semi‐LASER sequence with evenly distributed echo‐time (TE) was used, and a 90° J‐refocusing pulse was inserted at TE/2. Proton spectra were acquired at two TEs (30 and 68 ms), with and without the J‐refocused pulse. Data were processed in MATLAB and quantified with LCModel. Results The J‐refocused spectrum acquired at TE = 30 ms did not show any signal losses due to J‐modulation and had comparable spectral pattern to the one acquired with semi‐LASER using the minimum achievable TE. Higher signal amplitudes for glutamine, γ‐aminobutyric acid and glutathione led to more reliable quantification precision for these metabolites. The refocused signal intensities at TE = 68 ms were also unaffected by J‐modulation but were smaller than the signals at TE = 30 ms mainly due to transverse T2 relaxation of metabolites. Conclusion The proposed localized MRS sequence will be beneficial in both animal and human MRS studies when using ultra‐short TE is not possible while also providing more reliable quantification precision for J‐coupled metabolites.
ISSN:0740-3194
1522-2594
DOI:10.1002/mrm.28936