Parathyroid Carcinoma and Adenoma Co-existing in One Patient: Case Report and Comparative Proteomic Analysis

Background/Aim: The lack of specific parathyroid carcinoma (PC) biomarkers in clinical practice points out the importance of analyzing the proteomic signature of this cancer. We performed a comparative proteomic analysis of PC and parathyroid adenoma (PA) co-existing in the same patient. Patients an...

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Bibliographic Details
Published in:Cancer genomics & proteomics 2021-11, Vol.18 (6), p.781-796
Main Authors: CIREGIA, FEDERICA, CETANI, FILOMENA, PARDI, ELENA, SOGGIU, ALESSIO, PIRAS, CRISTIAN, ZALLOCCO, LORENZO, BORSARI, SIMONA, RONCI, MAURIZIO, CARUSO, VANNI, MARCOCCI, CLAUDIO, MAZZONI, MARIA ROSA, LUCACCHINI, ANTONIO, GIUSTI, LAURA
Format: Article
Language:English
Subjects:
2D-DIGE
Adenoma
Adenoma/diagnosis
Adenoma/pathology
Biochemistry
Biomarkers
Cancer
Cancer Research
case report
Case reports
Electrophoresis
Gel electrophoresis
Genetics
Human health sciences
Humans
Hydrolase
Male
Mass spectrometry
Mass spectroscopy
Middle Aged
Molecular Biology
Neoplasms
Neuroendocrine tumors
Oncologie
Oncology
Parathyroid
parathyroid adenoma
Parathyroid carcinoma
Parathyroid Neoplasms
Parathyroid Neoplasms/diagnosis
Parathyroid Neoplasms/pathology
Patients
Protein gene product 9.5
Proteins
proteomics
Proteomics/methods
Sciences de la santé humaine
Tumors
Two dimensional analysis
Ubiquitin
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Summary:Background/Aim: The lack of specific parathyroid carcinoma (PC) biomarkers in clinical practice points out the importance of analyzing the proteomic signature of this cancer. We performed a comparative proteomic analysis of PC and parathyroid adenoma (PA) co-existing in the same patient. Patients and Methods: PC and PA were taken from a 63-year-old patient. Using two-dimensional differential gel electrophoresis (2D-DIGE) coupled to mass spectrometry we examined the differences between PC and PA proteins. For validation, additional PC and PA samples were obtained from 10 patients. Western blot analysis was used to validate the difference of expression observed with 2D-DIGE analysis. Bioinfomatic analysis was performed using QIAGEN's Ingenuity Pathways Analysis (IPA) to determine the predominant canonical pathways and interaction networks involved. Results: Thirty-three differentially expressed proteins were identified in PC compared to PA. Among these, ubiquitin C-terminal hydrolase-L1 (UCH-L1) was highly overexpressed in PC. The result was confirmed by Western Blot analysis in additional PC samples. Conclusion: Our comparative proteomic analysis of co-existing neoplasms allowed detecting specific and peculiar differences between PC and PA overcoming population biological variability.
ISSN:1109-6535
1790-6295
1790-6245
DOI:10.21873/cgp.20297