Interleukin‐7 aggravates myocardial ischaemia/reperfusion injury by regulating macrophage infiltration and polarization

• Published in: Journal of cellular and molecular medicine 2021-11, Vol.25 (21), p.9939-9952
• Main Authors: Yan, Mengwen, Yang, Yaliu, Zhou, Ying, Yu, Changan, Li, Rui, Gong, Wei, Zheng, Jingang
• Format: Article
• Language: English
• Subjects: Animals; Apoptosis; Apoptosis - genetics; Biomarkers; Calcium-binding protein; Cardiomyocytes; Cell differentiation; Coronary vessels; Cytokines; Cytotoxicity; Disease Models, Animal; Disease Susceptibility; Electrocardiography; Gene Expression; Heart attacks; Heart Function Tests; Helper cells; Hemodynamics; Immunophenotyping; Infiltration; Inflammation; Interleukin-7 - genetics; Interleukin-7 - metabolism; interleukin‐7; ischaemia/reperfusion injury; Ischemia; Laboratory animals; Leukocyte migration; Lymphocytes T; macrophage; Macrophage Activation - immunology; Macrophages; Macrophages - immunology; Macrophages - metabolism; Macrophages - pathology; Medical research; Mice; Mice, Knockout; myocardial; Myocardial infarction; Myocardial Reperfusion Injury - diagnosis; Myocardial Reperfusion Injury - etiology; Myocardial Reperfusion Injury - metabolism; Myocytes, Cardiac - metabolism; Original; Polarization; Proteins; Reagents; Reperfusion; Supplements; Troponin; Troponin T; Veins & arteries
• ISSN: 1582-1838; 1582-4934
• DOI: 10.1111/jcmm.16335

Interleukin (IL)‐7 is known to enhance the macrophages cytotoxic activity and that macrophages play a pivotal role in the development and progression of myocardial ischaemia/reperfusion (I/R) injury. However, the effects of IL‐7 on macrophages infiltration and polarization in myocardial I/R injury are currently unclear. This study aimed to evaluate the effects of the IL‐7 expression on myocardial I/R injury and their relationship with macrophages. The data showed that IL‐7 expression in mouse heart tissue increases following I/R injury and that IL‐7 knockout or anti‐IL‐7 antibody treatment significantly improve I/R injury, including reduction in myocardial infarction area, a serum troponin T level decreases and an improvement in cardiac function. On the other hand, recombinant IL‐7 (rIL‐7) supplementation induces opposite effects and the anti‐IL‐7 antibody significantly reduces the cardiomyocyte apoptosis and macrophage infiltration. rIL‐7 cannot directly cause apoptosis, but it can induce cardiomyocyte apoptosis through macrophages, in addition to increase the macrophages migration in vitro. Anti‐IL‐7 antibody affects the cytokine production in T helper (Th) 1 and Th2 cells and also promotes the macrophages differentiation to M2 macrophages. However, anti‐IL‐7 antibody does not reduce the M1 macrophage number, and it only increases the ratio of M2/M1 macrophages in mice heart tissues after I/R injury. Taking together, these data reveal that IL‐7 plays an intensifying role in myocardial I/R injury by promoting cardiomyocyte apoptosis through the regulation of macrophage infiltration and polarization.