MicroRNA-139-5p Suppresses Cell Malignant Behaviors in Breast Cancer through Targeting MEX3A

Objective. The study was designed to evaluate the underlying mechanism of microRNA-139-5p in breast cancer (BC). Methods. Expression statuses of microRNA-139-5p and MEX3A were measured by qRT-PCR and western blotting. The anticancer effect of microRNA-139-5p in vitro was tested by a set of assays. I...

Full description

Saved in:
Bibliographic Details
Published in:Computational and mathematical methods in medicine 2021-11, Vol.2021, p.6591541-8
Main Authors: Chu, Jian, Li, Tangya, Li, Lei, Fan, Huiwen
Format: Article
Language:English
Subjects:
3' Untranslated Regions
Apoptosis - genetics
Breast Neoplasms - genetics
Breast Neoplasms - metabolism
Breast Neoplasms - pathology
Cell Line, Tumor
Cell Movement - genetics
Cell Proliferation - genetics
Computational Biology
Disease Progression
Down-Regulation
Female
Gene Expression Regulation, Neoplastic
Humans
MCF-7 Cells
MicroRNAs - genetics
MicroRNAs - metabolism
Neoplasm Invasiveness - genetics
Phosphoproteins - genetics
Phosphoproteins - metabolism
RNA-Binding Proteins - genetics
RNA-Binding Proteins - metabolism
Up-Regulation
Citations: Items that this one cites
Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:Objective. The study was designed to evaluate the underlying mechanism of microRNA-139-5p in breast cancer (BC). Methods. Expression statuses of microRNA-139-5p and MEX3A were measured by qRT-PCR and western blotting. The anticancer effect of microRNA-139-5p in vitro was tested by a set of assays. Interaction between microRNA-139-5p and MEX3A was validated by dual-luciferase detection. Results. MicroRNA-139-5p expression in BC cells was obviously low, while MEX3A was significantly overexpressed. MicroRNA-139-5p restrained proliferative, invasive, and migratory abilities of BC cells and increased apoptosis level of BC cells, while MEX3A exerted a promoting effect on BC cell growth. Dual-luciferase reporter detection confirmed that microRNA-139-5p bound to MEX3A 3′-UTR. Conclusions. MicroRNA-139-5p inhibited the development of BC by targeting MEX3A. MicroRNA-139-5p/MEX3A may be a target for BC therapy.
ISSN:1748-670X
1748-6718
DOI:10.1155/2021/6591541