Anti-inflammatory potential of Lactiplantibacillus plantarum IDCC 3501 and its safety evaluation

This study investigated the anti-inflammatory activity of Lactiplantibacillus plantarum IDCC 3501 isolated from kimchi (Korean fermented food) and its safety. When lipopolysaccharide (LPS)-induced RAW 264.7 macrophages were treated with cell-free supernatant from L. plantarum IDCC 3501, the mRNA exp...

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Bibliographic Details
Published in:Brazilian journal of microbiology 2021-12, Vol.52 (4), p.2299-2306
Main Authors: Yang, Soo-Yeon, Chae, Seung A, Bang, Won Yeong, Lee, Minjee, Ban, O-Hyun, Kim, Soo-Jung, Jung, Young Hoon, Yang, Jungwoo
Format: Article
Language:English
Subjects:
Acute toxicity
Amines
Animals
Anti-inflammatory agents
Anti-Inflammatory Agents - pharmacology
Antibiotic resistance
Antibiotics
Biogenic amines
Biomedical and Life Sciences
Cell viability
Fermentation
Fermented food
Food Microbiology
Food Microbiology - Research Paper
Food safety
Gene expression
Genomes
IL-1β
Inflammation
Interleukin 6
Lactic acid
Lactiplantibacillus plantarum
Lactobacillaceae - chemistry
Life Sciences
Lipopolysaccharides
Lipopolysaccharides - pharmacology
Macrophages
Medical Microbiology
Mice
Microbial Ecology
Microbial Genetics and Genomics
Microbial Sensitivity Tests
Microbiology
Mycology
Probiotics
RAW 264.7 Cells
Safety
Toxicity
Tumor necrosis factor-α
Virulence
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Summary:This study investigated the anti-inflammatory activity of Lactiplantibacillus plantarum IDCC 3501 isolated from kimchi (Korean fermented food) and its safety. When lipopolysaccharide (LPS)-induced RAW 264.7 macrophages were treated with cell-free supernatant from L. plantarum IDCC 3501, the mRNA expression level of inflammatory markers (i.e., TNF-α, IL-1β, and IL-6) was significantly reduced. In addition, the decreased cell viability by LPS was recovered and NO production in LPS-induced cell was also decreased. For the safety assessment, the genes responsible for antibiotic resistance and virulence were not detected from the genome analysis of this strain. Consistent with this, minimal inhibitory concentrations against various antibiotics, biogenic amines, and d -lactate production, as well as enzymatic and hemolysis activities, indicated that L. plantarum IDCC 3501 did not produce any harmful compounds during fermentation. Furthermore, no acute toxicity and mortality were observed in a murine mouse model. Based on our findings, L. plantarum IDCC 3501 is safe and beneficial for human consumption. Graphical abstract
ISSN:1517-8382
1678-4405
DOI:10.1007/s42770-021-00603-2