Microdilution susceptibility testing of amphotericin B, itraconazole, and voriconazole against clinical isolates of Aspergillus and Fusarium species

We compared the activities of amphotericin B, itraconazole, and voriconazole against clinical Aspergillus (n = 82) and Fusarium (n = 22) isolates by a microdilution method adopted from the National Committee for Clinical Laboratory Standards (NCCLS-M27A). RPMI 1640 (RPMI), RPMI 1640 supplemented to...

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Bibliographic Details
Published in:Journal of clinical microbiology 1999-12, Vol.37 (12), p.3946-3951
Main Authors: ARIKAN, S, LOZANO-CHIU, M, PAETZNICK, V, NANGIA, S, REX, J. H
Format: Article
Language:English
Subjects:
Amphotericin B - pharmacology
Antibiotics. Antiinfectious agents. Antiparasitic agents
Antifungal agents
Antifungal Agents - pharmacology
Aspergillosis - microbiology
Aspergillus - drug effects
Aspergillus - growth & development
Aspergillus - isolation & purification
Aspergillus flavus
Aspergillus fumigatus
Aspergillus nidulans
Aspergillus niger
Aspergillus terreus
Biological and medical sciences
Fusarium - drug effects
Fusarium - growth & development
Fusarium - isolation & purification
Fusarium oxysporum
Fusarium solani
Humans
Itraconazole - pharmacology
Medical sciences
Microbial Sensitivity Tests - methods
Mycology
Mycoses - microbiology
Pharmacology. Drug treatments
Pyrimidines - pharmacology
Triazoles - pharmacology
Voriconazole
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Summary:We compared the activities of amphotericin B, itraconazole, and voriconazole against clinical Aspergillus (n = 82) and Fusarium (n = 22) isolates by a microdilution method adopted from the National Committee for Clinical Laboratory Standards (NCCLS-M27A). RPMI 1640 (RPMI), RPMI 1640 supplemented to 2% glucose (RPMI-2), and antibiotic medium 3 supplemented to 2% glucose (AM3) were used as test media. MICs were determined after 24, 48, and 72 h. A narrow range of amphotericin B MICs was observed for Aspergillus isolates, with minor variations among species. MICs for Fusarium isolates were higher than those for Aspergillus isolates. MICs of itraconazole were prominently high for two previously defined itraconazole-resistant Aspergillus fumigatus isolates and Fusarium solani. Voriconazole showed good in vitro activity against itraconazole-resistant isolates, but the MICs of voriconazole for F. solani were high. RPMI was the most efficient medium for detection of itraconazole-resistant isolates, followed by RPMI-2. While the significance remains unclear, AM3 lowered the MICs, particularly those of amphotericin B.
ISSN:0095-1137
1098-660X
DOI:10.1128/jcm.37.12.3946-3951.1999