Degradability, in vitro fermentation parameters, and kinetic degradation of diets with increasing levels of forage and chitosan

• Published in: Translational animal science 2021-07, Vol.5 (3), p.txab086-txab086
• Main Authors: Jacaúna, Amanna Gonzaga, de Goes, Rafael Henrique de Tonissi e Buschinelli, Seno, Leonardo de Oliveira, Ítavo, Luis Carlos Vinhas, Gandra, Jefferson Rodrigues, da Silva, Nayara Gonçalves, Anschau, Douglas Gabriel, de Oliveira, Raquel Tenório, Bezerra, Leilson Rocha, Oliveira, Ronaldo Lopes
• Format: Article
• Language: English
• Subjects: Ammonia; Fatty acids; Natural gas; Rumen fermentation; Ruminant Nutrition
• ISSN: 2573-2102; 2573-2102
• DOI: 10.1093/tas/txab086

Abstract Chitosan is the second most important natural biopolymer in the world, extracted from crustaceans, shrimps, and crabs and can modulate rumen fermentation. Our hypothesis is that the addition of chitosan alters the fermentation patterns of different diets for ruminants. This study aimed to evaluate the effects of different levels of chitosan and forage on in vitro dry degradation kinetics and fermentation in a gas production system. The chitosan levels (0, 1625, 3,500, or 7,500 mg/kg of dry matter [DM]) were arranged in a completely randomized block design, and for in vitro ruminal fermentation assay, we used a split splot arrangement. Into the incubator, all chitosan levels were distributed in the four jars, and the forage levels varying on 100, 65, 50, 35, and 20 on DM basis. There was an interaction effect for chitosan and forage levels (P ≤ 0.05) on IVDMD; IVOMD. IVDCP and IVDNDF. Chitosan negatively affected IVDMD in all roughage levels evaluated. The pH and ammonia concentration present effect only for roughage levels and incubation hours. The chitosan did not change (P = 0.3631) the total short-chain fatty acid concentration (overall mean = 21.19 mmol/L) and the C2:C3 ratio (overall mean = 5.85). The IVDCP showed the same decreasing quadratic behavior (P < 0.0001). The increasing chitosan addition increases (P < 0.0001) the gas production and decreases (P < 0.0001) the lag time (parameter C) of diets with greater concentrate participation, characterizing greater efficiency in the degradability of the diet, confirming its potential use in diets for ruminants. Chitosan changes in vitro dry degradation kinetics and fermentation at the minimum dose of 1,722 mg/kg DM for all diets. The roughage level influenced the in vitro nutrients degradability and cumulative gas production.