Dental Composites – a Low-Dose Source of Bisphenol A?

• Published in: Physiological research 2020-09, Vol.69 (Suppl 2), p.S295-S304
• Main Authors: ŠIMKOVÁ, M., TICHÝ, A., DUŠKOVÁ, M., BRADNA, P.
• Format: Article
• Language: English
• Subjects: Bisphenol A; Body weight; Calibration; Chloride; Chromatography; Composite materials; Dental restorative materials; Endocrine disruptors; Liquid chromatography; Mass spectrometry; Mass spectroscopy; Methanol; Monomers; Polymerization; Scientific imaging
• ISSN: 0862-8408; 1802-9973
• DOI: 10.33549/physiolres.934518

Dental composite materials often contain monomers with bisphenol A (BPA) structure in their molecules, e.g. bisphenol-A glycidyl dimethacrylate (Bis-GMA). In this study, it was examined whether dental restorative composites could be a low-dose source of BPA or alternative bisphenols, which are known to have endocrine-disrupting effects. Bis-GMA-containing composites Charisma Classic (CC) and Filtek Ultimate Universal Restorative (FU) and “BPA-free” Charisma Diamond (CD) and Admira Fusion (AF) were examined. Specimens (diameter 6 mm, height 2 mm, n=5) were light-cured from one side for 20 s and stored at 37 °C in methanol which was periodically changed over 130 days to determine the kinetics of BPA release. BPA concentrations were measured using a dansyl chloride derivatization method with liquid chromatography – tandem mass spectrometry detection. The amounts of BPA were expressed in nanograms per gram of composite (ng/g). BPA release from Bis-GMA-containing CC and FU was significantly higher compared to “BPA-free” CD and AF. The highest 1-day release was detected with FU (15.4±0.8 ng/g), followed by CC (9.1±1.1 ng/g), AF (2.1±1.3 ng/g), and CD (1.6±0.8 ng/g), and the release gradually decreased over the examined period. Detected values were several orders of magnitude below the tolerable daily intake (4 µg/kg body weight/day). Alternative bisphenols were not detected. BPA was released even from “BPA-free” composites, although in significantly lower amounts than from Bis-GMA-containing composites. Despite incubation in methanol, detected amounts of BPA were substantially lower than current limits suggesting that dental composites should not pose a health risk if adequately polymerized.