Ultracompact fluorescence smartphone attachment using built-in optics for protoporphyrin-IX quantification in skin

Smartphone-based fluorescence imaging systems have the potential to provide convenient quantitative image guidance at the point of care. However, common approaches have required the addition of complex optical attachments, which reduce translation potential. In this study, a simple clip-on attachmen...

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Published in:Biomedical optics express 2021-11, Vol.12 (11), p.6995-7008
Main Authors: Hunt, Brady, Streeter, Samuel S, Ruiz, Alberto J, Chapman, M Shane, Pogue, Brian W
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description Smartphone-based fluorescence imaging systems have the potential to provide convenient quantitative image guidance at the point of care. However, common approaches have required the addition of complex optical attachments, which reduce translation potential. In this study, a simple clip-on attachment appropriate for fluorescence imaging of protoporphyrin-IX (PpIX) in skin was designed using the built-in light source and ultrawide camera sensor of a smartphone. Software control for image acquisition and quantitative analysis was developed using the 10-bit video capability of the phone. Optical performance was characterized using PpIX in liquid tissue phantoms and endogenously produced PpIX in mice and human skin. The proposed system achieves a very compact form factor (
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title Ultracompact fluorescence smartphone attachment using built-in optics for protoporphyrin-IX quantification in skin
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