Upregulation of ENDOU in cytotrophoblasts from placenta complicated with preeclampsia and fetal growth restriction

Placental hypoplasia is associated with the pathophysiology of fetal growth restriction and preeclampsia. The placenta consists of differentiated trophoblasts, including cytotrophoblasts, syncytio­trophoblasts, and extravillous trophoblasts. Cytotrophoblasts are thought to have stem-like characteris...

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Bibliographic Details
Published in:Journal of Clinical Biochemistry and Nutrition 2021, Vol.69(3), pp.280-285
Main Authors: Nomoto, Masataka, Kotani, Tomomi, Miki, Rika, Ushida, Takafumi, Imai, Kenji, Iitani, Yukako, Tano, Sho, Wang, Jingwen, Moriyama, Yoshinori, Kobayashi, Tomoko, Mimura, Nobuko, Iriyama, Takayuki, Kikkawa, Fumitaka, Kajiyama, Hiroaki
Format: Article
Language:English
Subjects:
Coronaviruses
cytotrophoblast
Endonuclease
Fetuses
Homology
Hypoplasia
LC-MS/MS
Liquid chromatography
Mass spectrometry
Mass spectroscopy
Original
Placenta
Pre-eclampsia
Preeclampsia
Proteins
Severe acute respiratory syndrome coronavirus 2
Trophoblasts
Viral diseases
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Summary:Placental hypoplasia is associated with the pathophysiology of fetal growth restriction and preeclampsia. The placenta consists of differentiated trophoblasts, including cytotrophoblasts, syncytio­trophoblasts, and extravillous trophoblasts. Cytotrophoblasts are thought to have stem-like characteristics and the ability to differentiate into syncytiotrophoblasts and extravillous trophoblasts. However, it is poorly understood whether isolated cytotrophoblasts derived from hypoplastic placentas have specific features compared with those in normal placentas. This study aimed to determine the features of cytotrophoblasts in hypoplastic placentas. Differentially expressed proteins between isolated cytotrophoblasts from hypoplastic placenta with fetal growth restriction and those from the normal placenta were determined by liquid chromatography-tandem mass spectrometry. Among 6,802 proteins, 1,253 and 2,129 proteins were more than 2-fold upregulated and downregulated, respectively. Among them, ENDOU (endonuclease, poly(U) specific), which has high homology with the coronavirus endoribonuclease nonstructural protein 15 (Nsp15), showed a significantly increased expression in cytotrophoblasts from the placenta with fetal growth restriction related to preeclampsia compared with those in normal control placenta. These results provide insight into the pathological mechanisms of placental hypoplasia and additional information on preeclamptic symptoms in cases of SARS-CoV-2 infected placenta, although further investigation is needed.
ISSN:0912-0009
1880-5086
DOI:10.3164/jcbn.21-37