Extracellular Vesicles of Adipose-Derived Stem Cells Promote the Healing of Traumatized Achilles Tendons

Healing of ruptured tendons remains a clinical challenge because of its slow progress and relatively weak mechanical force at an early stage. Extracellular vesicles (EVs) derived from mesenchymal stem cells (MSCs) have therapeutic potential for tissue regeneration. In this study, we isolated EVs fro...

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Bibliographic Details
Published in:International journal of molecular sciences 2021-11, Vol.22 (22), p.12373
Main Authors: Chen, Shih-Heng, Chen, Zhi-Yu, Lin, Ya-Hsuan, Chen, Shih-Hsien, Chou, Pang-Yun, Kao, Huang-Kai, Lin, Feng-Huei
Format: Article
Language:English
Subjects:
Achilles tendon
Achilles Tendon - injuries
Adipocytes - cytology
Adipocytes - metabolism
Adipose Tissue - cytology
Adipose Tissue - metabolism
Animals
Biglycan - genetics
Biglycan - metabolism
Biomarkers - metabolism
Bone marrow
Cell Differentiation
Cell growth
Cell Movement
Cell Proliferation
Collagen
Decorin
Decorin - genetics
Decorin - metabolism
Extracellular vesicles
Extracellular Vesicles - chemistry
Extracellular Vesicles - transplantation
Female
Gene Expression
Healing
Histology
Inflammation
Mechanical properties
Mesenchyme
Proteins
Rabbits
Range of motion
Stem cells
Stem Cells - chemistry
Stem Cells - cytology
Stem Cells - metabolism
Tendon Injuries - metabolism
Tendon Injuries - pathology
Tendon Injuries - therapy
Tendons
Tenocytes - cytology
Tenocytes - metabolism
Tissue engineering
Trauma
Treatment Outcome
Vesicles
Wound Healing - physiology
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Summary:Healing of ruptured tendons remains a clinical challenge because of its slow progress and relatively weak mechanical force at an early stage. Extracellular vesicles (EVs) derived from mesenchymal stem cells (MSCs) have therapeutic potential for tissue regeneration. In this study, we isolated EVs from adipose-derived stem cells (ADSCs) and evaluated their ability to promote tendon regeneration. Our results indicated that ADSC-EVs significantly enhanced the proliferation and migration of tenocytes in vitro. To further study the roles of ADSC-EVs in tendon regeneration, ADSC-EVs were used in Achilles tendon repair in rabbits. The mechanical strength, histology, and protein expression in the injured tendon tissues significantly improved 4 weeks after ADSC-EV treatment. Decorin and biglycan were significantly upregulated in comparison to the untreated controls. In summary, ADSC-EVs stimulated the proliferation and migration of tenocytes and improved the mechanical strength of repaired tendons, suggesting that ADSC-EV treatment is a potential highly potent therapeutic strategy for tendon injuries.
ISSN:1422-0067
1661-6596
1422-0067
DOI:10.3390/ijms222212373