Bone Regeneration of a 3D-Printed Alloplastic and Particulate Xenogenic Graft with rhBMP-2

• Published in: International journal of molecular sciences 2021-11, Vol.22 (22), p.12518
• Main Authors: Ryu, Ji-In, Yang, Byoung-Eun, Yi, Sang-Min, Choi, Hyo Geun, On, Sung-Woon, Hong, Seok Jin, Lim, Ho-Kyung, Byun, Soo-Hwan
• Format: Article
• Language: English
• Subjects: 3-D printers; Animals; Biomedical materials; Bone grafts; Bone growth; Bone morphogenetic protein 2; Bone Morphogenetic Protein 2 - genetics; Bone Morphogenetic Protein 2 - pharmacology; Bone Regeneration - drug effects; Bone Regeneration - genetics; Bone Substitutes - pharmacology; Bones; Calcium phosphates; Calcium Phosphates - pharmacology; Customization; Dogs; Durapatite - pharmacology; Grafting; Growth factors; Humans; Hydroxyapatite; Infections; Jaw - pathology; Jaw Abnormalities - genetics; Jaw Abnormalities - pathology; Jaw Abnormalities - therapy; Maxillofacial Development - genetics; Osteogenesis; Printing, Three-Dimensional; Recombinant Proteins - genetics; Recombinant Proteins - pharmacology; Regeneration; Regeneration (physiology); Skin & tissue grafts; Skull - drug effects; Skull - growth & development; Skull - pathology; Transforming Growth Factor beta - genetics; Transforming Growth Factor beta - pharmacology; Transplants & implants; Tricalcium phosphate; Xenografts; Xenotransplantation
• ISSN: 1422-0067; 1661-6596; 1422-0067
• DOI: 10.3390/ijms222212518

This study aimed to evaluate the bone regeneration capacity of a customized alloplastic material and xenograft with recombinant human bone morphogenetic protein-2 (rhBMP-2). We prepared hydroxyapatite (HA)/tricalcium phosphate (TCP) pure ceramic bone blocks made using a 3D printing system and added rhBMP-2 to both materials. In eight beagle dogs, a total of 32 defects were created on the lower jaws. The defective sites of the negative control group were left untreated (N group; 8 defects), and those in the positive control group were filled with particle-type Bio-Oss (P group; 12 defects). The defect sites in the experimental group were filled with 3D-printed synthetic bone blocks (3D group; 12 defects). Radiographic and histological evaluations were performed after healing periods of 6 and 12 weeks and showed no significant difference in new bone formation and total bone between the P and 3D groups. The 3D-printed custom HA/TCP graft with rhBMP-2 showed bone regeneration effects similar to that of particulate Bio-Oss with rhBMP-2. Through further study and development, the application of 3D-printed customized alloplastic grafts will be extended to various fields of bone regeneration.