Optogenetic control of cancer cell survival in ChR2‐transfected HeLa cells

Optogenetics is a molecular biological technique involving transfection of cells with photosensitive proteins and the subsequent study of their biological effects. The aim of this study was to evaluate the effect of blue light on the survival of HeLa cells, transfected with channelrhodopsin‐2 (ChR2)...

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Bibliographic Details
Published in:International journal of experimental pathology 2021-12, Vol.102 (6), p.242-248
Main Authors: Córdova, Claudio, Lozano, Carlo, Rodríguez, Belén, Marchant, Ivanny, Zúñiga, Rodrigo, Ochova, Paola, Olivero, Pablo, González‐Arriagada, Wilfredo Alejandro
Format: Article
Language:English
Subjects:
Biological effects
Calcium
Calcium (intracellular)
Calcium - metabolism
Cell culture
Cell Cycle - physiology
Cell death
Cell survival
Cell Survival - physiology
Channelrhodopsins - genetics
Channelrhodopsins - metabolism
Cytoplasm
Flow cytometry
Fluorescence
Genetics
Green fluorescent protein
HeLa Cells
Humans
Information processing
Iodides
Isomerization
Light effects
light‐activated protein
Medical research
Optics
Optogenetics
Original
Photosensitivity
physiology
Polymerase chain reaction
Propidium iodide
Proteins
Rhodamine
rhodopsin
Statistical analysis
Stimulation
Survival
Transfection
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Summary:Optogenetics is a molecular biological technique involving transfection of cells with photosensitive proteins and the subsequent study of their biological effects. The aim of this study was to evaluate the effect of blue light on the survival of HeLa cells, transfected with channelrhodopsin‐2 (ChR2). HeLa wild‐type cells were transfected with a plasmid that contained the gene for ChR2. Transfection and channel function were evaluated by real‐time polymerase chain reaction (RT‐PCR), fluorescence imaging using green fluorescent protein (GFP) and flow cytometry for intracellular calcium changes using a Fura Red probe. We developed a platform for optogenetic stimulation for use within the cell culture incubator. Different stimulation procedures using blue light (467 nm) were applied for up to 24 h. Cell survival was determined by flow cytometry using propidium iodide and rhodamine probes. Change in cell survival showed a statistically significant (p 
ISSN:0959-9673
1365-2613
DOI:10.1111/iep.12426