Healing of colonic anastomosis in rats under obstructive ileus conditions

BACKGROUNDThe anastomosis leak in colon resections is a crucial post-operative complication with significant morbidity and mortality. Methods: Forty (40) Wistar rats were allocated in two groups. In SHAM group only anastomosis was performed. In ILEUS group anastomosis was performed following one day...

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Published in:Discoveries (Craiova, Romania) Romania), 2021-06, Vol.9 (2), p.e129-e129
Main Authors: Despoudi, Kalliopi, Mantzoros, Ioannis, Ioannidis, Orestis, Loutzidou, Lydia, Christidis, Panagiotis, Chatzakis, Christos, Gkasdaris, Grigorios, Raptis, Dimitrios, Pramateftakis, Manousos George, Angelopoulos, Stamatios, Zaraboukas, Thomas, Koliakos, George, Tsalis, Konstantinos
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container_issue 2
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container_title Discoveries (Craiova, Romania)
container_volume 9
creator Despoudi, Kalliopi
Mantzoros, Ioannis
Ioannidis, Orestis
Loutzidou, Lydia
Christidis, Panagiotis
Chatzakis, Christos
Gkasdaris, Grigorios
Raptis, Dimitrios
Pramateftakis, Manousos George
Angelopoulos, Stamatios
Zaraboukas, Thomas
Koliakos, George
Tsalis, Konstantinos
description BACKGROUNDThe anastomosis leak in colon resections is a crucial post-operative complication with significant morbidity and mortality. Methods: Forty (40) Wistar rats were allocated in two groups. In SHAM group only anastomosis was performed. In ILEUS group anastomosis was performed following one day of ileus. Animals in both groups were subdivided in two groups according to the day they were sacrificed, 4th or 8th post-operative day. A number of variables between the groups were estimated. RESULTSBody weight loss was higher following obstructive ileus on both days. Adhesion score in 4th and 8th post-operative day was higher in ILEUS1, ILEUS2 groups compared to SHAM1, SHAM2 groups respectively (p
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Methods: Forty (40) Wistar rats were allocated in two groups. In SHAM group only anastomosis was performed. In ILEUS group anastomosis was performed following one day of ileus. Animals in both groups were subdivided in two groups according to the day they were sacrificed, 4th or 8th post-operative day. A number of variables between the groups were estimated. RESULTSBody weight loss was higher following obstructive ileus on both days. Adhesion score in 4th and 8th post-operative day was higher in ILEUS1, ILEUS2 groups compared to SHAM1, SHAM2 groups respectively (p&lt;0.001 for both). Neovascularization decreased following obstructive ileus compared to control on the 4th day (ILEUS1 vs. SHAM1, p=0.038). Bursting pressure was lower in ILEUS2 group than SHAM2 group (p&lt;0.001). The number of fibroblasts decreased following obstructive ileus compared to control on the 4th and 8th day (ILEUS1 vs. SHAM1, p=0.001, ILEUS2 vs SHAM2, p=0.016). Hydroxyproline concentration was decreased in ILEUS2 group compared to SHAM2 group (p&lt;0.001). CONCLUSIONSThe balance of collagenolysis and collagenogenesis plays a decisive role in the healing of anastomoses following bowel obstruction. Under those circumstances, anastomosis' bursting pressure is reduced owning to decreased neovascularization, reduced fibroblast presence and lower hydroxyproline concertation. In our study, local inflammation, neocollagen concentration and collagenase activity were not associated with this adverse effect. However, further research should delineate the mechanisms of healing of colonic anastomoses and identify those factors that can improve our outcomes.</description><identifier>ISSN: 2359-7232</identifier><identifier>EISSN: 2359-7232</identifier><identifier>DOI: 10.15190/d.2021.8</identifier><identifier>PMID: 34849396</identifier><language>eng</language><publisher>Applied Systems srl</publisher><subject>Original</subject><ispartof>Discoveries (Craiova, Romania), 2021-06, Vol.9 (2), p.e129-e129</ispartof><rights>Copyright © 2021, Despoudi K et al., Applied Systems and Discoveries Journals. 2021</rights><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c1978-da3e4398e75601fc7955f03c18a738bf65f9386f145e9f3ceb6c51c758c463e23</citedby></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC8627191/pdf/$$EPDF$$P50$$Gpubmedcentral$$Hfree_for_read</linktopdf><linktohtml>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC8627191/$$EHTML$$P50$$Gpubmedcentral$$Hfree_for_read</linktohtml><link.rule.ids>230,314,727,780,784,885,27923,27924,53790,53792</link.rule.ids></links><search><creatorcontrib>Despoudi, Kalliopi</creatorcontrib><creatorcontrib>Mantzoros, Ioannis</creatorcontrib><creatorcontrib>Ioannidis, Orestis</creatorcontrib><creatorcontrib>Loutzidou, Lydia</creatorcontrib><creatorcontrib>Christidis, Panagiotis</creatorcontrib><creatorcontrib>Chatzakis, Christos</creatorcontrib><creatorcontrib>Gkasdaris, Grigorios</creatorcontrib><creatorcontrib>Raptis, Dimitrios</creatorcontrib><creatorcontrib>Pramateftakis, Manousos George</creatorcontrib><creatorcontrib>Angelopoulos, Stamatios</creatorcontrib><creatorcontrib>Zaraboukas, Thomas</creatorcontrib><creatorcontrib>Koliakos, George</creatorcontrib><creatorcontrib>Tsalis, Konstantinos</creatorcontrib><creatorcontrib>Department of Biochemistry, School of Medicine, Aristotle University of Thessaloniki, Greece</creatorcontrib><creatorcontrib>Department of Pathology, School of Medicine, Faculty of Health Sciences, Aristotle University of Thessaloniki, Greece</creatorcontrib><creatorcontrib>4th Academic Department of Surgery, School of Medicine, Faculty of Health Sciences, Aristotle University of Thessaloniki, Greece</creatorcontrib><title>Healing of colonic anastomosis in rats under obstructive ileus conditions</title><title>Discoveries (Craiova, Romania)</title><description>BACKGROUNDThe anastomosis leak in colon resections is a crucial post-operative complication with significant morbidity and mortality. Methods: Forty (40) Wistar rats were allocated in two groups. In SHAM group only anastomosis was performed. In ILEUS group anastomosis was performed following one day of ileus. Animals in both groups were subdivided in two groups according to the day they were sacrificed, 4th or 8th post-operative day. A number of variables between the groups were estimated. RESULTSBody weight loss was higher following obstructive ileus on both days. Adhesion score in 4th and 8th post-operative day was higher in ILEUS1, ILEUS2 groups compared to SHAM1, SHAM2 groups respectively (p&lt;0.001 for both). Neovascularization decreased following obstructive ileus compared to control on the 4th day (ILEUS1 vs. SHAM1, p=0.038). Bursting pressure was lower in ILEUS2 group than SHAM2 group (p&lt;0.001). The number of fibroblasts decreased following obstructive ileus compared to control on the 4th and 8th day (ILEUS1 vs. SHAM1, p=0.001, ILEUS2 vs SHAM2, p=0.016). Hydroxyproline concentration was decreased in ILEUS2 group compared to SHAM2 group (p&lt;0.001). CONCLUSIONSThe balance of collagenolysis and collagenogenesis plays a decisive role in the healing of anastomoses following bowel obstruction. Under those circumstances, anastomosis' bursting pressure is reduced owning to decreased neovascularization, reduced fibroblast presence and lower hydroxyproline concertation. In our study, local inflammation, neocollagen concentration and collagenase activity were not associated with this adverse effect. 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Methods: Forty (40) Wistar rats were allocated in two groups. In SHAM group only anastomosis was performed. In ILEUS group anastomosis was performed following one day of ileus. Animals in both groups were subdivided in two groups according to the day they were sacrificed, 4th or 8th post-operative day. A number of variables between the groups were estimated. RESULTSBody weight loss was higher following obstructive ileus on both days. Adhesion score in 4th and 8th post-operative day was higher in ILEUS1, ILEUS2 groups compared to SHAM1, SHAM2 groups respectively (p&lt;0.001 for both). Neovascularization decreased following obstructive ileus compared to control on the 4th day (ILEUS1 vs. SHAM1, p=0.038). Bursting pressure was lower in ILEUS2 group than SHAM2 group (p&lt;0.001). The number of fibroblasts decreased following obstructive ileus compared to control on the 4th and 8th day (ILEUS1 vs. SHAM1, p=0.001, ILEUS2 vs SHAM2, p=0.016). Hydroxyproline concentration was decreased in ILEUS2 group compared to SHAM2 group (p&lt;0.001). CONCLUSIONSThe balance of collagenolysis and collagenogenesis plays a decisive role in the healing of anastomoses following bowel obstruction. Under those circumstances, anastomosis' bursting pressure is reduced owning to decreased neovascularization, reduced fibroblast presence and lower hydroxyproline concertation. In our study, local inflammation, neocollagen concentration and collagenase activity were not associated with this adverse effect. However, further research should delineate the mechanisms of healing of colonic anastomoses and identify those factors that can improve our outcomes.</abstract><pub>Applied Systems srl</pub><pmid>34849396</pmid><doi>10.15190/d.2021.8</doi><oa>free_for_read</oa></addata></record>
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title Healing of colonic anastomosis in rats under obstructive ileus conditions
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