Jinwujiangu Capsule Treats Fibroblast-Like Synoviocytes of Rheumatoid Arthritis by Inhibiting Pyroptosis via the NLRP3/CAPSES/GSDMD Pathway

Jinwujiangu capsule (JWJGC) is a traditional Chinese medicine formula used to treat rheumatoid arthritis (RA). However, whether its mechanism is associated with pyroptosis remains unclear. In this study, the ability of JWJGC to inhibit the growth of fibroblast-like synoviocytes of RA (RA-FLS) throug...

Full description

Saved in:
Bibliographic Details
Published in:Evidence-based complementary and alternative medicine 2021, Vol.2021, p.4836992-10
Main Authors: Ling, Yi, Xiao, Mao, Huang, Zhao-Wei, Xu, Hui, Huang, Fang-Qin, Ren, Ni-Na, Chen, Chang-Ming, Lu, Dao Min, Yao, Xue-Ming, Xiao, Li-Na, Ma, Wu-Kai
Format: Article
Language:English
Subjects:
Antibodies
Apoptosis
Caspase-1
Cell proliferation
Chinese medicine
Cytokines
Drug dosages
Fibroblasts
Flow cytometry
Hospitals
IL-1β
Immunohistochemistry
Interleukin 18
Pyroptosis
Rabbits
Rheumatoid arthritis
Synoviocytes
Traditional Chinese medicine
Citations: Items that this one cites
Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:Jinwujiangu capsule (JWJGC) is a traditional Chinese medicine formula used to treat rheumatoid arthritis (RA). However, whether its mechanism is associated with pyroptosis remains unclear. In this study, the ability of JWJGC to inhibit the growth of fibroblast-like synoviocytes of RA (RA-FLS) through pyroptosis was evaluated. The cells isolated from patients with RA were identified by hematoxylin and eosin (H&E) staining, immunohistochemistry, and flow cytometry. After RA-FLS were treated with different concentrations of JWJGC-containing serum, the cell proliferation inhibition rate, expression of caspase-1/3/4/5, NOD-like receptor protein 3 (NLRP3), gasdermin-D (GSDMD), and apoptosis-associated speck-like protein containing a CARD (ASC), concentrations of interleukin-1β (IL-1β) and interleukin-18 (IL-18), the activity of lactic dehydrogenase (LDH), and pyroptosis were evaluated. The results showed that JWJGC increased the proliferative inhibition rate, decreased the expression of caspase-1/3/4/5, GSDMD, NLRP3, and ASC, suppressed the expression of IL-1β and IL-18, induced the activity of LDH, and downregulated the number of double-positive FITC anti-caspase-1 and PI. Generally, our findings suggest that JWJGC can regulate NLRP3/CAPSES/GSDMD in treating RA-FLS through pyroptosis.
ISSN:1741-427X
1741-4288
DOI:10.1155/2021/4836992