Serum Exosomal microRNA Profiling in AIDS Complicated with Talaromyces marneffei Infection

In order to find the early diagnostic markers of AIDS combined with TM infection, we detected and analyzed the serum exosomal miRNAs of AIDS patients with or without TM infection. We profiled the expression levels of miRNAs via RNA sequencing in serum exosomes from the pooled samples of 17 AIDS pati...

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Bibliographic Details
Published in:Infection and drug resistance 2021-01, Vol.14, p.4931-4948
Main Authors: Ning, Qiu-Yue, Liu, Na, Wu, Ji-Zhou, Hu, Die-Fei, Wei, Qi, Zhou, Jin-Ai, Zou, Jun, Zang, Ning, Li, Guo-Jian
Format: Article
Language:English
Subjects:
Acquired immune deficiency syndrome
AIDS
AIDS (Disease)
Analysis
Bioinformatics
Cyclic adenylic acid
Cyclic AMP
Cyclic GMP
Cytomegalovirus
Development and progression
DNA-directed RNA polymerase
Exosomes
Gene regulation
Genetic transcription
Health aspects
HIV
HIV patients
Human immunodeficiency virus
MAP kinase
MicroRNA
MicroRNAs
miRNA
Mortality
Original Research
Polymerase chain reaction
RNA sequencing
Signal transduction
Transcription
Transforming growth factors
Wnt protein
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Summary:In order to find the early diagnostic markers of AIDS combined with TM infection, we detected and analyzed the serum exosomal miRNAs of AIDS patients with or without TM infection. We profiled the expression levels of miRNAs via RNA sequencing in serum exosomes from the pooled samples of 17 AIDS patients combined without TM infection and 15 AIDS combined with TM infection patients. For external validation, we validated these results using real-time quantification polymerase chain reaction (qRT-PCR) in an independent cohort of 35 AIDS patients combined without TM infection and 33 AIDS combined with TM infection patients. Finally, bioinformatics was used to predict the target genes and pathways of meaningful miRNAs. A total of 131 serum exosomal miRNAs including 73 up-regulated and 59 down-regulated miRNAs were found to be differentially expressed (log2FC≥1 and FDR
ISSN:1178-6973
1178-6973
DOI:10.2147/IDR.S338321