Biomarkers and Mechanism Analysis for Polygoni Multiflori Radix Preparata-Induced Liver Injury by UHPLC-Q-TOF-MS-Based Metabolomics

Background. Polygonum Multiflorum Radix Preparata (PMP), prepared from Polygonum multiflorum Thunb. (PM), is traditionally valued for its liver and kidney-tonifying effects. However, the previous studies showed that PMP was hepatotoxic, which limited its clinical use. Unfortunately, the potential he...

Full description

Saved in:
Bibliographic Details
Published in:Evidence-based complementary and alternative medicine 2021, Vol.2021, p.7677392-10
Main Authors: Wang, Liming, Wang, Zhida, Xing, Yanchao, Liu, Erwei, Gao, Xiumei, Wang, Linlin, Fu, Zhifei
Format: Article
Language:English
Subjects:
Anticoagulants
Bile acids
Biomarkers
Cholestasis
Chromatography
Discriminant analysis
Ethanol
Hepatotoxicity
Kidneys
Laboratory animals
Lipid metabolism
Liver
Mass spectrometry
Metabolism
Metabolites
Metabolomics
NMR
Nuclear magnetic resonance
Oral administration
Scientific imaging
Software
Statistical analysis
Citations: Items that this one cites
Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:Background. Polygonum Multiflorum Radix Preparata (PMP), prepared from Polygonum multiflorum Thunb. (PM), is traditionally valued for its liver and kidney-tonifying effects. However, the previous studies showed that PMP was hepatotoxic, which limited its clinical use. Unfortunately, the potential hepatotoxic ingredients and the molecular mechanism are still uncertain. Objective. The aim of this study was to find out potential biomarkers of hepatotoxicity using metabolomics profile. Materials and Methods. 60% ethanol extract of PMP (PMPE) was prepared. Subsequently, an untargeted metabolomics technology in combination with ROC curve analysis method was applied to investigate the alteration of plasma metabolites in rats after oral administration of PMPE (40 g/kg/d) for 28 days. Results. Compared to the control group, the significant difference in metabolic profiling was observed in the PMPE-induced liver injury group, and sixteen highly specific biomarkers were identified. These metabolites were mainly enriched into bile acids, lipids, and energy metabolisms, indicating that PMPE-induced liver injury could be related to cholestasis and dysregulated lipid metabolism. Conclusions. This study is contributed to understand the potential pathogenesis of PMP-induced liver injury. The metabonomic method may be a valuable tool for the clinical diagnosis of PMP-induced liver injury.
ISSN:1741-427X
1741-4288
DOI:10.1155/2021/7677392