Three LHPP gene‐targeting co‐expressed microRNAs (microRNA‐765, microRNA‐21, and microRNA‐144) promote proliferation, epithelial‐mesenchymal transition, invasion, and are independent prognostic biomarkers in renal cell carcinomas patients

Background Renal cell carcinoma (RCC) is one of the highly malignant tumors in the world. Global Cancer Statistics 2020 estimated that there were 179,368 deaths from kidney tumors. Therefore, exploring the prognostic biomarkers of RCC is of great significance for RCC patients. This study aims to exp...

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Bibliographic Details
Published in:Journal of clinical laboratory analysis 2021-12, Vol.35 (12), p.e24077-n/a
Main Authors: Meng, Kexin, Li, Zongda, Cui, Xiaoying
Format: Article
Language:English
Subjects:
Aged
Antibodies
Binding sites
Biomarkers
Biomarkers, Tumor - genetics
Cancer
Carcinoma, Renal Cell - genetics
Carcinoma, Renal Cell - mortality
Carcinoma, Renal Cell - pathology
Cell Line, Tumor
Cell proliferation
Cell Proliferation - genetics
Epithelial-Mesenchymal Transition - genetics
Female
Fibroblasts
Fluorescence in situ hybridization
Gene Expression Regulation, Neoplastic
Humans
Immunohistochemistry
Inorganic Pyrophosphatase - genetics
Inorganic Pyrophosphatase - metabolism
Kidney cancer
Kidney Neoplasms - genetics
Kidney Neoplasms - mortality
Kidney Neoplasms - pathology
Localization
Male
Medical prognosis
Medical research
Mesenchyme
MicroRNAs
MicroRNAs - genetics
Middle Aged
miRNA
Phosphohistidine
phospholysine phosphohistidine inorganic pyrophosphate phosphatase
Prognosis
Proteins
Renal cell carcinoma
Reporter gene
Survival Analysis
Tumors
Western blotting
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Summary:Background Renal cell carcinoma (RCC) is one of the highly malignant tumors in the world. Global Cancer Statistics 2020 estimated that there were 179,368 deaths from kidney tumors. Therefore, exploring the prognostic biomarkers of RCC is of great significance for RCC patients. This study aims to explore the potential mechanism and prognostic value of phospholysine phosphohistidine inorganic pyrophosphate phosphatase (LHPP) gene‐targeting co‐expression microRNAs in RCC patients. Methods A total of 60 RCC patients were included. Quantitative real‐time PCR (qRT‐PCR), western blotting, and immunohistochemistry were used for LHPP, microRNA‐765, microRNA‐21, and microRNA‐144 levels evaluation. Cell Counting Kit‐8 assay, dual‐luciferase reporter gene assay, invasion assay, and RNA fluorescence in situ hybridization were used for functional analyses. Results Compared with adjacent tissues, LHPP levels in cancer tissues were significantly increased (p 
ISSN:0887-8013
1098-2825
DOI:10.1002/jcla.24077