Mir-204 Regulates LPS-Induced A549 Cell Damage by Targeting FOXK2

Objective. To assess whether miR-204 and HA affect A549 cell injury induced by lipopolysaccharide. Material and Methods. A549 cells were treated with hirsutanol A, and cell damage was induced by LPS followed by analysis of cell proliferation by CCK-8, cell apoptosis by flow cytometry, apoptosis-rela...

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Published in:Journal of healthcare engineering 2021, Vol.2021, p.7404671-10
Main Authors: Li, Shufen, Zhao, Lifen, Li, Xujiong, Shang, Gaiping, Gao, Lijing, Song, Zhuohui, Li, Ting
Format: Article
Language:English
Subjects:
A549 Cells
Apoptosis - genetics
Forkhead Transcription Factors
Humans
Lipopolysaccharides - pharmacology
MicroRNAs - genetics
MicroRNAs - metabolism
NF-kappa B - metabolism
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Summary:Objective. To assess whether miR-204 and HA affect A549 cell injury induced by lipopolysaccharide. Material and Methods. A549 cells were treated with hirsutanol A, and cell damage was induced by LPS followed by analysis of cell proliferation by CCK-8, cell apoptosis by flow cytometry, apoptosis-related protein expression by western blot, downstream target of miR-20 by dual-luciferase reporter gene, and inflammatory factors by ELISA and PCR. Results. LPS can significantly inhibit the viability of A549 cells, induce cell apoptosis, and promote the release of IL-6, IL-1β, and TNF-α, while HA pretreatment can target FOXK2 by upregulating miR-204 levels, thereby alleviating apoptosis and promoting cell viability and at the same time inhibiting the release of inflammatory factors by inhibiting the activation of NF-κB. Conclusions. miR-204 participates in the protection of HA acute lung injury by targeting FOXK2.
ISSN:2040-2295
2040-2309
DOI:10.1155/2021/7404671