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The Effects of Vitamin D on the Expression of IL-33 and Its Receptor ST2 in Skin Cells; Potential Implication for Psoriasis
Interleukin 33 (IL-33) belongs to the IL-1 family and is produced constitutively by epithelial and endothelial cells of various organs, such as the skin. It takes part in the maintenance of tissue homeostasis, repair, and immune response, including activation of Th2 lymphocytes. Its involvement in p...
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Published in: | International journal of molecular sciences 2021-11, Vol.22 (23), p.12907 |
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creator | Wierzbicka, Justyna M Piotrowska, Anna Purzycka-Bohdan, Dorota Olszewska, Anna Nowak, Joanna I Szczerkowska-Dobosz, Aneta Nedoszytko, Bogusław Nowicki, Roman J Żmijewski, Michał A |
description | Interleukin 33 (IL-33) belongs to the IL-1 family and is produced constitutively by epithelial and endothelial cells of various organs, such as the skin. It takes part in the maintenance of tissue homeostasis, repair, and immune response, including activation of Th2 lymphocytes. Its involvement in pathogenesis of several inflammatory diseases including psoriasis was also suggested, but this is not fully understood. The aim of the study was to investigate expression of IL-33 and its receptor, ST2, in psoriasis, and the effects of the active form of vitamin D (1,25(OH)
D
) on their expression in skin cells. Here we examined mRNA and protein profiles of IL-33 and ST2 in 18 psoriatic patients and healthy volunteers by qPCR and immunostaining techniques. Potential effects of 1,25(OH)
D
and its receptor (VDR) on the expression of IL-33 and ST2 were tested in cultured keratinocytes, melanocytes, fibroblasts, and basal cell carcinoma cells. It was shown that 1,25(OH)
D
effectively stimulated expression of IL-33 and its receptor ST2's mRNAs in a time-dependent manner, in keratinocytes and to the lesser extends in melanocytes, but not in fibroblasts. Furthermore, the effect of vitamin D on expression of IL-33 and ST2 was VDR-dependent. Finally, we demonstrated that the expression of mRNA for IL-33 was mainly elevated in the psoriatic skin but not in its margin. Interestingly, ST2 mRNA was downregulated in psoriatic lesion compared to both marginal tissue as well as healthy skin. Our data indicated that vitamin D can modulate IL-33 signaling, opening up new perspectives for our understanding of the mechanism of vitamin D action in psoriasis therapy. |
doi_str_mv | 10.3390/ijms222312907 |
format | article |
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D
) on their expression in skin cells. Here we examined mRNA and protein profiles of IL-33 and ST2 in 18 psoriatic patients and healthy volunteers by qPCR and immunostaining techniques. Potential effects of 1,25(OH)
D
and its receptor (VDR) on the expression of IL-33 and ST2 were tested in cultured keratinocytes, melanocytes, fibroblasts, and basal cell carcinoma cells. It was shown that 1,25(OH)
D
effectively stimulated expression of IL-33 and its receptor ST2's mRNAs in a time-dependent manner, in keratinocytes and to the lesser extends in melanocytes, but not in fibroblasts. Furthermore, the effect of vitamin D on expression of IL-33 and ST2 was VDR-dependent. Finally, we demonstrated that the expression of mRNA for IL-33 was mainly elevated in the psoriatic skin but not in its margin. Interestingly, ST2 mRNA was downregulated in psoriatic lesion compared to both marginal tissue as well as healthy skin. Our data indicated that vitamin D can modulate IL-33 signaling, opening up new perspectives for our understanding of the mechanism of vitamin D action in psoriasis therapy.</description><identifier>ISSN: 1422-0067</identifier><identifier>ISSN: 1661-6596</identifier><identifier>EISSN: 1422-0067</identifier><identifier>DOI: 10.3390/ijms222312907</identifier><identifier>PMID: 34884710</identifier><language>eng</language><publisher>Switzerland: MDPI AG</publisher><subject>Adolescent ; Apoptosis ; Basal cell carcinoma ; Calciferol ; Calcitriol ; Cancer ; Case-Control Studies ; Cell activation ; Comorbidity ; Cytokines ; Emotional disorders ; Endothelial cells ; Enzymes ; Fibroblasts ; Gene expression ; Homeostasis ; Humans ; Immune response ; Immune system ; Immunology ; Inflammatory diseases ; Interleukin 1 ; Interleukin-1 Receptor-Like 1 Protein - genetics ; Interleukin-1 Receptor-Like 1 Protein - metabolism ; Interleukin-33 - genetics ; Interleukin-33 - metabolism ; Keratinocytes ; Keratinocytes - drug effects ; Keratinocytes - metabolism ; Localization ; Lymphocytes ; Lymphocytes T ; Melanocytes ; Metabolism ; Metabolites ; Mood disorders ; mRNA ; Obesity ; Organs ; Pathogenesis ; Proteins ; Psoriasis ; Psoriasis - drug therapy ; Psoriasis - metabolism ; Psoriasis - pathology ; Skin ; Skin - drug effects ; Skin - metabolism ; Skin diseases ; Vitamin D ; Vitamin D - pharmacology ; Vitamin D receptors ; Vitamin deficiency ; Vitamins - pharmacology</subject><ispartof>International journal of molecular sciences, 2021-11, Vol.22 (23), p.12907</ispartof><rights>2021 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/). Notwithstanding the ProQuest Terms and Conditions, you may use this content in accordance with the terms of the License.</rights><rights>2021 by the authors. 2021</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c415t-63ba8a8b6f2816616eef413b88f0940e5194f946c013d22bfbb779957847d06a3</citedby><cites>FETCH-LOGICAL-c415t-63ba8a8b6f2816616eef413b88f0940e5194f946c013d22bfbb779957847d06a3</cites><orcidid>0000-0002-6089-8030 ; 0000-0003-4768-1387 ; 0000-0001-6109-0685 ; 0000-0002-2672-0474 ; 0000-0003-2206-3531 ; 0000-0002-6286-0693</orcidid></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.proquest.com/docview/2608128586/fulltextPDF?pq-origsite=primo$$EPDF$$P50$$Gproquest$$Hfree_for_read</linktopdf><linktohtml>$$Uhttps://www.proquest.com/docview/2608128586?pq-origsite=primo$$EHTML$$P50$$Gproquest$$Hfree_for_read</linktohtml><link.rule.ids>230,314,727,780,784,885,25752,27923,27924,37011,37012,44589,53790,53792,74897</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/34884710$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Wierzbicka, Justyna M</creatorcontrib><creatorcontrib>Piotrowska, Anna</creatorcontrib><creatorcontrib>Purzycka-Bohdan, Dorota</creatorcontrib><creatorcontrib>Olszewska, Anna</creatorcontrib><creatorcontrib>Nowak, Joanna I</creatorcontrib><creatorcontrib>Szczerkowska-Dobosz, Aneta</creatorcontrib><creatorcontrib>Nedoszytko, Bogusław</creatorcontrib><creatorcontrib>Nowicki, Roman J</creatorcontrib><creatorcontrib>Żmijewski, Michał A</creatorcontrib><title>The Effects of Vitamin D on the Expression of IL-33 and Its Receptor ST2 in Skin Cells; Potential Implication for Psoriasis</title><title>International journal of molecular sciences</title><addtitle>Int J Mol Sci</addtitle><description>Interleukin 33 (IL-33) belongs to the IL-1 family and is produced constitutively by epithelial and endothelial cells of various organs, such as the skin. It takes part in the maintenance of tissue homeostasis, repair, and immune response, including activation of Th2 lymphocytes. Its involvement in pathogenesis of several inflammatory diseases including psoriasis was also suggested, but this is not fully understood. The aim of the study was to investigate expression of IL-33 and its receptor, ST2, in psoriasis, and the effects of the active form of vitamin D (1,25(OH)
D
) on their expression in skin cells. Here we examined mRNA and protein profiles of IL-33 and ST2 in 18 psoriatic patients and healthy volunteers by qPCR and immunostaining techniques. Potential effects of 1,25(OH)
D
and its receptor (VDR) on the expression of IL-33 and ST2 were tested in cultured keratinocytes, melanocytes, fibroblasts, and basal cell carcinoma cells. It was shown that 1,25(OH)
D
effectively stimulated expression of IL-33 and its receptor ST2's mRNAs in a time-dependent manner, in keratinocytes and to the lesser extends in melanocytes, but not in fibroblasts. Furthermore, the effect of vitamin D on expression of IL-33 and ST2 was VDR-dependent. Finally, we demonstrated that the expression of mRNA for IL-33 was mainly elevated in the psoriatic skin but not in its margin. Interestingly, ST2 mRNA was downregulated in psoriatic lesion compared to both marginal tissue as well as healthy skin. Our data indicated that vitamin D can modulate IL-33 signaling, opening up new perspectives for our understanding of the mechanism of vitamin D action in psoriasis therapy.</description><subject>Adolescent</subject><subject>Apoptosis</subject><subject>Basal cell carcinoma</subject><subject>Calciferol</subject><subject>Calcitriol</subject><subject>Cancer</subject><subject>Case-Control Studies</subject><subject>Cell activation</subject><subject>Comorbidity</subject><subject>Cytokines</subject><subject>Emotional disorders</subject><subject>Endothelial cells</subject><subject>Enzymes</subject><subject>Fibroblasts</subject><subject>Gene expression</subject><subject>Homeostasis</subject><subject>Humans</subject><subject>Immune response</subject><subject>Immune system</subject><subject>Immunology</subject><subject>Inflammatory diseases</subject><subject>Interleukin 1</subject><subject>Interleukin-1 Receptor-Like 1 Protein - genetics</subject><subject>Interleukin-1 Receptor-Like 1 Protein - metabolism</subject><subject>Interleukin-33 - genetics</subject><subject>Interleukin-33 - metabolism</subject><subject>Keratinocytes</subject><subject>Keratinocytes - drug effects</subject><subject>Keratinocytes - metabolism</subject><subject>Localization</subject><subject>Lymphocytes</subject><subject>Lymphocytes T</subject><subject>Melanocytes</subject><subject>Metabolism</subject><subject>Metabolites</subject><subject>Mood disorders</subject><subject>mRNA</subject><subject>Obesity</subject><subject>Organs</subject><subject>Pathogenesis</subject><subject>Proteins</subject><subject>Psoriasis</subject><subject>Psoriasis - drug therapy</subject><subject>Psoriasis - metabolism</subject><subject>Psoriasis - pathology</subject><subject>Skin</subject><subject>Skin - drug effects</subject><subject>Skin - metabolism</subject><subject>Skin diseases</subject><subject>Vitamin D</subject><subject>Vitamin D - pharmacology</subject><subject>Vitamin D receptors</subject><subject>Vitamin deficiency</subject><subject>Vitamins - pharmacology</subject><issn>1422-0067</issn><issn>1661-6596</issn><issn>1422-0067</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2021</creationdate><recordtype>article</recordtype><sourceid>PIMPY</sourceid><recordid>eNpdkUtLxDAUhYMovpduJeDGTTWPNk0QBBlHHRhQdHQb0k6iGdumJhlR_PNmfKFuklzOl8M9HAB2MDqgVKBDO2sDIYRiIlC5BNZxTkiGECuXf73XwEYIM4QIJYVYBWs05zwvMVoHb5MHDYfG6DoG6Ay8s1G1toOn0HUwLrSX3usQbBqTPBpnlELVTeEo8de61n10Ht5MCEyfbh7TMdBNE47glYu6i1Y1cNT2ja1VXFiYBF8F560KNmyBFaOaoLe_7k1wezacDC6y8eX5aHAyzuocFzFjtFJc8YoZwjFjmGltckwrzg0SOdIFFrkROasRplNCKlNVZSlEUaaIU8QU3QTHn779vGr1tE57edXI3ttW-VfplJV_lc4-yHv3LDkrSsZEMtj_MvDuaa5DlK0NdcqpOu3mQRKGeEGZ-ED3_qEzN_ddivdBYcILzhKVfVK1dyF4bX6WwUguapV_ak387u8EP_R3j_Qde6mc8A</recordid><startdate>20211129</startdate><enddate>20211129</enddate><creator>Wierzbicka, Justyna M</creator><creator>Piotrowska, Anna</creator><creator>Purzycka-Bohdan, Dorota</creator><creator>Olszewska, Anna</creator><creator>Nowak, Joanna I</creator><creator>Szczerkowska-Dobosz, Aneta</creator><creator>Nedoszytko, Bogusław</creator><creator>Nowicki, Roman J</creator><creator>Żmijewski, Michał A</creator><general>MDPI AG</general><general>MDPI</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>3V.</scope><scope>7X7</scope><scope>7XB</scope><scope>88E</scope><scope>8FI</scope><scope>8FJ</scope><scope>8FK</scope><scope>8G5</scope><scope>ABUWG</scope><scope>AFKRA</scope><scope>AZQEC</scope><scope>BENPR</scope><scope>CCPQU</scope><scope>DWQXO</scope><scope>FYUFA</scope><scope>GHDGH</scope><scope>GNUQQ</scope><scope>GUQSH</scope><scope>K9.</scope><scope>M0S</scope><scope>M1P</scope><scope>M2O</scope><scope>MBDVC</scope><scope>PIMPY</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>PRINS</scope><scope>Q9U</scope><scope>7X8</scope><scope>5PM</scope><orcidid>https://orcid.org/0000-0002-6089-8030</orcidid><orcidid>https://orcid.org/0000-0003-4768-1387</orcidid><orcidid>https://orcid.org/0000-0001-6109-0685</orcidid><orcidid>https://orcid.org/0000-0002-2672-0474</orcidid><orcidid>https://orcid.org/0000-0003-2206-3531</orcidid><orcidid>https://orcid.org/0000-0002-6286-0693</orcidid></search><sort><creationdate>20211129</creationdate><title>The Effects of Vitamin D on the Expression of IL-33 and Its Receptor ST2 in Skin Cells; Potential Implication for Psoriasis</title><author>Wierzbicka, Justyna M ; Piotrowska, Anna ; Purzycka-Bohdan, Dorota ; Olszewska, Anna ; Nowak, Joanna I ; Szczerkowska-Dobosz, Aneta ; Nedoszytko, Bogusław ; Nowicki, Roman J ; Żmijewski, Michał A</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c415t-63ba8a8b6f2816616eef413b88f0940e5194f946c013d22bfbb779957847d06a3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2021</creationdate><topic>Adolescent</topic><topic>Apoptosis</topic><topic>Basal cell carcinoma</topic><topic>Calciferol</topic><topic>Calcitriol</topic><topic>Cancer</topic><topic>Case-Control Studies</topic><topic>Cell activation</topic><topic>Comorbidity</topic><topic>Cytokines</topic><topic>Emotional disorders</topic><topic>Endothelial cells</topic><topic>Enzymes</topic><topic>Fibroblasts</topic><topic>Gene expression</topic><topic>Homeostasis</topic><topic>Humans</topic><topic>Immune response</topic><topic>Immune system</topic><topic>Immunology</topic><topic>Inflammatory diseases</topic><topic>Interleukin 1</topic><topic>Interleukin-1 Receptor-Like 1 Protein - genetics</topic><topic>Interleukin-1 Receptor-Like 1 Protein - metabolism</topic><topic>Interleukin-33 - genetics</topic><topic>Interleukin-33 - metabolism</topic><topic>Keratinocytes</topic><topic>Keratinocytes - drug effects</topic><topic>Keratinocytes - metabolism</topic><topic>Localization</topic><topic>Lymphocytes</topic><topic>Lymphocytes T</topic><topic>Melanocytes</topic><topic>Metabolism</topic><topic>Metabolites</topic><topic>Mood disorders</topic><topic>mRNA</topic><topic>Obesity</topic><topic>Organs</topic><topic>Pathogenesis</topic><topic>Proteins</topic><topic>Psoriasis</topic><topic>Psoriasis - drug therapy</topic><topic>Psoriasis - metabolism</topic><topic>Psoriasis - pathology</topic><topic>Skin</topic><topic>Skin - drug effects</topic><topic>Skin - metabolism</topic><topic>Skin diseases</topic><topic>Vitamin D</topic><topic>Vitamin D - pharmacology</topic><topic>Vitamin D receptors</topic><topic>Vitamin deficiency</topic><topic>Vitamins - pharmacology</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Wierzbicka, Justyna M</creatorcontrib><creatorcontrib>Piotrowska, Anna</creatorcontrib><creatorcontrib>Purzycka-Bohdan, Dorota</creatorcontrib><creatorcontrib>Olszewska, Anna</creatorcontrib><creatorcontrib>Nowak, Joanna I</creatorcontrib><creatorcontrib>Szczerkowska-Dobosz, Aneta</creatorcontrib><creatorcontrib>Nedoszytko, Bogusław</creatorcontrib><creatorcontrib>Nowicki, Roman J</creatorcontrib><creatorcontrib>Żmijewski, Michał A</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>ProQuest Central (Corporate)</collection><collection>Health & Medical Collection</collection><collection>ProQuest Central (purchase pre-March 2016)</collection><collection>Medical Database (Alumni Edition)</collection><collection>Hospital Premium Collection</collection><collection>Hospital Premium Collection (Alumni Edition)</collection><collection>ProQuest Central (Alumni) (purchase pre-March 2016)</collection><collection>Research Library (Alumni Edition)</collection><collection>ProQuest Central (Alumni Edition)</collection><collection>ProQuest Central</collection><collection>ProQuest Central Essentials</collection><collection>ProQuest Central</collection><collection>ProQuest One Community College</collection><collection>ProQuest Central Korea</collection><collection>Health Research Premium Collection</collection><collection>Health Research Premium Collection (Alumni)</collection><collection>ProQuest Central Student</collection><collection>Research Library Prep</collection><collection>ProQuest Health & Medical Complete (Alumni)</collection><collection>Health & Medical Collection (Alumni Edition)</collection><collection>PML(ProQuest Medical Library)</collection><collection>ProQuest_Research Library</collection><collection>Research Library (Corporate)</collection><collection>ProQuest - 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It takes part in the maintenance of tissue homeostasis, repair, and immune response, including activation of Th2 lymphocytes. Its involvement in pathogenesis of several inflammatory diseases including psoriasis was also suggested, but this is not fully understood. The aim of the study was to investigate expression of IL-33 and its receptor, ST2, in psoriasis, and the effects of the active form of vitamin D (1,25(OH)
D
) on their expression in skin cells. Here we examined mRNA and protein profiles of IL-33 and ST2 in 18 psoriatic patients and healthy volunteers by qPCR and immunostaining techniques. Potential effects of 1,25(OH)
D
and its receptor (VDR) on the expression of IL-33 and ST2 were tested in cultured keratinocytes, melanocytes, fibroblasts, and basal cell carcinoma cells. It was shown that 1,25(OH)
D
effectively stimulated expression of IL-33 and its receptor ST2's mRNAs in a time-dependent manner, in keratinocytes and to the lesser extends in melanocytes, but not in fibroblasts. Furthermore, the effect of vitamin D on expression of IL-33 and ST2 was VDR-dependent. Finally, we demonstrated that the expression of mRNA for IL-33 was mainly elevated in the psoriatic skin but not in its margin. Interestingly, ST2 mRNA was downregulated in psoriatic lesion compared to both marginal tissue as well as healthy skin. Our data indicated that vitamin D can modulate IL-33 signaling, opening up new perspectives for our understanding of the mechanism of vitamin D action in psoriasis therapy.</abstract><cop>Switzerland</cop><pub>MDPI AG</pub><pmid>34884710</pmid><doi>10.3390/ijms222312907</doi><orcidid>https://orcid.org/0000-0002-6089-8030</orcidid><orcidid>https://orcid.org/0000-0003-4768-1387</orcidid><orcidid>https://orcid.org/0000-0001-6109-0685</orcidid><orcidid>https://orcid.org/0000-0002-2672-0474</orcidid><orcidid>https://orcid.org/0000-0003-2206-3531</orcidid><orcidid>https://orcid.org/0000-0002-6286-0693</orcidid><oa>free_for_read</oa></addata></record> |
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subjects | Adolescent Apoptosis Basal cell carcinoma Calciferol Calcitriol Cancer Case-Control Studies Cell activation Comorbidity Cytokines Emotional disorders Endothelial cells Enzymes Fibroblasts Gene expression Homeostasis Humans Immune response Immune system Immunology Inflammatory diseases Interleukin 1 Interleukin-1 Receptor-Like 1 Protein - genetics Interleukin-1 Receptor-Like 1 Protein - metabolism Interleukin-33 - genetics Interleukin-33 - metabolism Keratinocytes Keratinocytes - drug effects Keratinocytes - metabolism Localization Lymphocytes Lymphocytes T Melanocytes Metabolism Metabolites Mood disorders mRNA Obesity Organs Pathogenesis Proteins Psoriasis Psoriasis - drug therapy Psoriasis - metabolism Psoriasis - pathology Skin Skin - drug effects Skin - metabolism Skin diseases Vitamin D Vitamin D - pharmacology Vitamin D receptors Vitamin deficiency Vitamins - pharmacology |
title | The Effects of Vitamin D on the Expression of IL-33 and Its Receptor ST2 in Skin Cells; Potential Implication for Psoriasis |
url | http://sfxeu10.hosted.exlibrisgroup.com/loughborough?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2025-01-11T10%3A04%3A19IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-proquest_pubme&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=The%20Effects%20of%20Vitamin%20D%20on%20the%20Expression%20of%20IL-33%20and%20Its%20Receptor%20ST2%20in%20Skin%20Cells;%20Potential%20Implication%20for%20Psoriasis&rft.jtitle=International%20journal%20of%20molecular%20sciences&rft.au=Wierzbicka,%20Justyna%20M&rft.date=2021-11-29&rft.volume=22&rft.issue=23&rft.spage=12907&rft.pages=12907-&rft.issn=1422-0067&rft.eissn=1422-0067&rft_id=info:doi/10.3390/ijms222312907&rft_dat=%3Cproquest_pubme%3E2608128586%3C/proquest_pubme%3E%3Cgrp_id%3Ecdi_FETCH-LOGICAL-c415t-63ba8a8b6f2816616eef413b88f0940e5194f946c013d22bfbb779957847d06a3%3C/grp_id%3E%3Coa%3E%3C/oa%3E%3Curl%3E%3C/url%3E&rft_id=info:oai/&rft_pqid=2608128586&rft_id=info:pmid/34884710&rfr_iscdi=true |