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The Effects of Vitamin D on the Expression of IL-33 and Its Receptor ST2 in Skin Cells; Potential Implication for Psoriasis

Interleukin 33 (IL-33) belongs to the IL-1 family and is produced constitutively by epithelial and endothelial cells of various organs, such as the skin. It takes part in the maintenance of tissue homeostasis, repair, and immune response, including activation of Th2 lymphocytes. Its involvement in p...

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Published in:International journal of molecular sciences 2021-11, Vol.22 (23), p.12907
Main Authors: Wierzbicka, Justyna M, Piotrowska, Anna, Purzycka-Bohdan, Dorota, Olszewska, Anna, Nowak, Joanna I, Szczerkowska-Dobosz, Aneta, Nedoszytko, Bogusław, Nowicki, Roman J, Żmijewski, Michał A
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cited_by cdi_FETCH-LOGICAL-c415t-63ba8a8b6f2816616eef413b88f0940e5194f946c013d22bfbb779957847d06a3
cites cdi_FETCH-LOGICAL-c415t-63ba8a8b6f2816616eef413b88f0940e5194f946c013d22bfbb779957847d06a3
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container_issue 23
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container_title International journal of molecular sciences
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creator Wierzbicka, Justyna M
Piotrowska, Anna
Purzycka-Bohdan, Dorota
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Nowak, Joanna I
Szczerkowska-Dobosz, Aneta
Nedoszytko, Bogusław
Nowicki, Roman J
Żmijewski, Michał A
description Interleukin 33 (IL-33) belongs to the IL-1 family and is produced constitutively by epithelial and endothelial cells of various organs, such as the skin. It takes part in the maintenance of tissue homeostasis, repair, and immune response, including activation of Th2 lymphocytes. Its involvement in pathogenesis of several inflammatory diseases including psoriasis was also suggested, but this is not fully understood. The aim of the study was to investigate expression of IL-33 and its receptor, ST2, in psoriasis, and the effects of the active form of vitamin D (1,25(OH) D ) on their expression in skin cells. Here we examined mRNA and protein profiles of IL-33 and ST2 in 18 psoriatic patients and healthy volunteers by qPCR and immunostaining techniques. Potential effects of 1,25(OH) D and its receptor (VDR) on the expression of IL-33 and ST2 were tested in cultured keratinocytes, melanocytes, fibroblasts, and basal cell carcinoma cells. It was shown that 1,25(OH) D effectively stimulated expression of IL-33 and its receptor ST2's mRNAs in a time-dependent manner, in keratinocytes and to the lesser extends in melanocytes, but not in fibroblasts. Furthermore, the effect of vitamin D on expression of IL-33 and ST2 was VDR-dependent. Finally, we demonstrated that the expression of mRNA for IL-33 was mainly elevated in the psoriatic skin but not in its margin. Interestingly, ST2 mRNA was downregulated in psoriatic lesion compared to both marginal tissue as well as healthy skin. Our data indicated that vitamin D can modulate IL-33 signaling, opening up new perspectives for our understanding of the mechanism of vitamin D action in psoriasis therapy.
doi_str_mv 10.3390/ijms222312907
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It takes part in the maintenance of tissue homeostasis, repair, and immune response, including activation of Th2 lymphocytes. Its involvement in pathogenesis of several inflammatory diseases including psoriasis was also suggested, but this is not fully understood. The aim of the study was to investigate expression of IL-33 and its receptor, ST2, in psoriasis, and the effects of the active form of vitamin D (1,25(OH) D ) on their expression in skin cells. Here we examined mRNA and protein profiles of IL-33 and ST2 in 18 psoriatic patients and healthy volunteers by qPCR and immunostaining techniques. Potential effects of 1,25(OH) D and its receptor (VDR) on the expression of IL-33 and ST2 were tested in cultured keratinocytes, melanocytes, fibroblasts, and basal cell carcinoma cells. It was shown that 1,25(OH) D effectively stimulated expression of IL-33 and its receptor ST2's mRNAs in a time-dependent manner, in keratinocytes and to the lesser extends in melanocytes, but not in fibroblasts. Furthermore, the effect of vitamin D on expression of IL-33 and ST2 was VDR-dependent. Finally, we demonstrated that the expression of mRNA for IL-33 was mainly elevated in the psoriatic skin but not in its margin. Interestingly, ST2 mRNA was downregulated in psoriatic lesion compared to both marginal tissue as well as healthy skin. 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Potential Implication for Psoriasis</atitle><jtitle>International journal of molecular sciences</jtitle><addtitle>Int J Mol Sci</addtitle><date>2021-11-29</date><risdate>2021</risdate><volume>22</volume><issue>23</issue><spage>12907</spage><pages>12907-</pages><issn>1422-0067</issn><issn>1661-6596</issn><eissn>1422-0067</eissn><abstract>Interleukin 33 (IL-33) belongs to the IL-1 family and is produced constitutively by epithelial and endothelial cells of various organs, such as the skin. It takes part in the maintenance of tissue homeostasis, repair, and immune response, including activation of Th2 lymphocytes. Its involvement in pathogenesis of several inflammatory diseases including psoriasis was also suggested, but this is not fully understood. The aim of the study was to investigate expression of IL-33 and its receptor, ST2, in psoriasis, and the effects of the active form of vitamin D (1,25(OH) D ) on their expression in skin cells. Here we examined mRNA and protein profiles of IL-33 and ST2 in 18 psoriatic patients and healthy volunteers by qPCR and immunostaining techniques. Potential effects of 1,25(OH) D and its receptor (VDR) on the expression of IL-33 and ST2 were tested in cultured keratinocytes, melanocytes, fibroblasts, and basal cell carcinoma cells. It was shown that 1,25(OH) D effectively stimulated expression of IL-33 and its receptor ST2's mRNAs in a time-dependent manner, in keratinocytes and to the lesser extends in melanocytes, but not in fibroblasts. Furthermore, the effect of vitamin D on expression of IL-33 and ST2 was VDR-dependent. Finally, we demonstrated that the expression of mRNA for IL-33 was mainly elevated in the psoriatic skin but not in its margin. Interestingly, ST2 mRNA was downregulated in psoriatic lesion compared to both marginal tissue as well as healthy skin. 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source ProQuest - Publicly Available Content Database; PubMed Central
subjects Adolescent
Apoptosis
Basal cell carcinoma
Calciferol
Calcitriol
Cancer
Case-Control Studies
Cell activation
Comorbidity
Cytokines
Emotional disorders
Endothelial cells
Enzymes
Fibroblasts
Gene expression
Homeostasis
Humans
Immune response
Immune system
Immunology
Inflammatory diseases
Interleukin 1
Interleukin-1 Receptor-Like 1 Protein - genetics
Interleukin-1 Receptor-Like 1 Protein - metabolism
Interleukin-33 - genetics
Interleukin-33 - metabolism
Keratinocytes
Keratinocytes - drug effects
Keratinocytes - metabolism
Localization
Lymphocytes
Lymphocytes T
Melanocytes
Metabolism
Metabolites
Mood disorders
mRNA
Obesity
Organs
Pathogenesis
Proteins
Psoriasis
Psoriasis - drug therapy
Psoriasis - metabolism
Psoriasis - pathology
Skin
Skin - drug effects
Skin - metabolism
Skin diseases
Vitamin D
Vitamin D - pharmacology
Vitamin D receptors
Vitamin deficiency
Vitamins - pharmacology
title The Effects of Vitamin D on the Expression of IL-33 and Its Receptor ST2 in Skin Cells; Potential Implication for Psoriasis
url http://sfxeu10.hosted.exlibrisgroup.com/loughborough?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2025-01-11T10%3A04%3A19IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-proquest_pubme&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=The%20Effects%20of%20Vitamin%20D%20on%20the%20Expression%20of%20IL-33%20and%20Its%20Receptor%20ST2%20in%20Skin%20Cells;%20Potential%20Implication%20for%20Psoriasis&rft.jtitle=International%20journal%20of%20molecular%20sciences&rft.au=Wierzbicka,%20Justyna%20M&rft.date=2021-11-29&rft.volume=22&rft.issue=23&rft.spage=12907&rft.pages=12907-&rft.issn=1422-0067&rft.eissn=1422-0067&rft_id=info:doi/10.3390/ijms222312907&rft_dat=%3Cproquest_pubme%3E2608128586%3C/proquest_pubme%3E%3Cgrp_id%3Ecdi_FETCH-LOGICAL-c415t-63ba8a8b6f2816616eef413b88f0940e5194f946c013d22bfbb779957847d06a3%3C/grp_id%3E%3Coa%3E%3C/oa%3E%3Curl%3E%3C/url%3E&rft_id=info:oai/&rft_pqid=2608128586&rft_id=info:pmid/34884710&rfr_iscdi=true