Distinct Fastigial Output Channels and Their Impact on Temporal Lobe Seizures

• Published in: The Journal of neuroscience 2021-12, Vol.41 (49), p.10091-10107
• Main Authors: Streng, Martha L, Tetzlaff, Madison R, Krook-Magnuson, Esther
• Format: Article
• Language: English
• Subjects: Animals; Brain stem; Cerebellum; Cerebellum - physiopathology; Channels; Convulsions & seizures; Epilepsy; Epilepsy, Temporal Lobe - physiopathology; Female; Hippocampus; Kainic acid; Male; Mice; Mice, Inbred C57BL; Modulation; Motor task performance; Neural Pathways - physiopathology; Neurons; Neurons - physiology; Reticular formation; Seizures; Seizures - physiopathology; Stimulation; Superior colliculus; Temporal lobe; Thalamus; Therapeutic applications; Viruses
• ISSN: 0270-6474; 1529-2401
• DOI: 10.1523/JNEUROSCI.0683-21.2021

Despite being canonically considered a motor control structure, the cerebellum is increasingly recognized for important roles in processes beyond this traditional framework, including seizure suppression. Excitatory fastigial neurons project to a large number of downstream targets, and it is unclear whether this broad targeting underlies seizure suppression, or whether a specific output may be sufficient. To address this question, we used the intrahippocampal kainic acid mouse model of temporal lobe epilepsy, male and female animals, and a dual-virus approach to selectively label and manipulate fastigial outputs. We examined fastigial neurons projecting to the superior colliculus, medullary reticular formation, and central lateral nucleus of the thalamus, and found that these comprise largely nonoverlapping populations of neurons that send collaterals to unique sets of additional, somewhat overlapping, thalamic and brainstem regions. We found that neither optogenetic stimulation of superior colliculus nor reticular formation output channels attenuated hippocampal seizures. In contrast, on-demand stimulation of fastigial neurons targeting the central lateral nucleus robustly inhibited seizures. Our results indicate that fastigial control of hippocampal seizures does not require simultaneous modulation of many fastigial output channels. Rather, selective modulation of the fastigial output channel to the central lateral thalamus, specifically, is sufficient for seizure control. More broadly, our data highlight the concept of specific cerebellar output channels, whereby discrete cerebellar nucleus neurons project to specific aggregates of downstream targets, with important consequences for therapeutic interventions. The cerebellum has an emerging relationship with nonmotor systems and may represent a powerful target for therapeutic intervention in temporal lobe epilepsy. We find, as previously reported, that fastigial neurons project to numerous brain regions via largely segregated output channels, and that projection targets cannot be predicted simply by somatic locations within the nucleus. We further find that on-demand optogenetic excitation of fastigial neurons projecting to the central lateral nucleus of the thalamus-but not fastigial neurons projecting to the reticular formation, superior colliculus, or ventral lateral thalamus-is sufficient to attenuate hippocampal seizures.