Nanchung and Inactive define pore properties of the native auditory transduction channel in Drosophila

Auditory transduction is mediated by chordotonal (Cho) neurons in Drosophila larvae, but the molecular identity of the mechanotransduction (MET) channel is elusive. Here, we established a whole-cell recording system of Cho neurons and showed that two transient receptor potential vanilloid (TRPV) cha...

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Bibliographic Details
Published in:Proceedings of the National Academy of Sciences - PNAS 2021-12, Vol.118 (49), p.1-11
Main Authors: Li, Bingxue, Li, Songling, Zheng, Honglan, Yan, Zhiqiang
Format: Article
Language:English
Subjects:
Action Potentials - physiology
Amino Acid Sequence
Animals
Biological Sciences
Cell Line
Channel gating
Drosophila
Drosophila melanogaster
Drosophila Proteins - genetics
Drosophila Proteins - metabolism
Electrophysiological Phenomena
Fruit flies
Gene Expression Regulation
Hearing
Insects
Ion channels
Ion Channels - genetics
Ion Channels - metabolism
Kinetics
Larva
Larvae
Mechanotransduction
Mechanotransduction, Cellular - physiology
Mutation
Neurons
Neurons - physiology
Patch-Clamp Techniques
Point Mutation
Pore formation
Transient Receptor Potential Channels - genetics
Transient Receptor Potential Channels - metabolism
Transient receptor potential proteins
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Summary:Auditory transduction is mediated by chordotonal (Cho) neurons in Drosophila larvae, but the molecular identity of the mechanotransduction (MET) channel is elusive. Here, we established a whole-cell recording system of Cho neurons and showed that two transient receptor potential vanilloid (TRPV) channels, Nanchung (NAN) and Inactive (IAV), are essential for MET currents in Cho neurons. NAN and IAV form active ion channels when expressed simultaneously in S2 cells. Point mutations in the pore region of NAN-IAV change the reversal potential of the MET currents. Particularly, residues 857 through 990 in the IAV carboxyl terminus regulate the kinetics of MET currents in Cho neurons. In addition, TRPN channel NompC contributes to the adaptation of auditory transduction currents independent of its ion-conduction function. These results indicate that NAN-IAV, rather than NompC, functions as essential pore-forming subunits of the native auditory transduction channel in Drosophila and provide insights into the gating mechanism of MET currents in Cho neurons.
ISSN:0027-8424
1091-6490
DOI:10.1073/pnas.2106459118