Functionalisation of Fe3 O4 nanoparticles by 2‐((pyrazol‐4‐yl) methylene) hydrazinecarbothioamide enhances the apoptosis of human breast cancer MCF‐7 cells

• Published in: IET nanobiotechnology 2020-08, Vol.14 (6), p.508-518
• Main Authors: Izadpanah, Mohammad Reza, Salehzadeh, Ali, Zaefizadeh, Mohammad, Nikpasand, Mohammad
• Format: Article
• Language: English
• Subjects: (3‐chloropropyl) trimethoxysilane; 2‐((pyrazol‐4‐yl) methylene) hydrazinecarbothioamide; anticancer properties; apoptosis test; biochemistry; biomedical materials; cancer; caspase‐3 activity assay; cell cycle analysis; cell rest induction; cellular biophysics; chemotherapy drugs; cytotoxicity effect; drugs; dynamic light scattering; electrokinetic effects; energy‐dispersive X‐ray spectrometry; enzymes; Fe3 O4; Fe3 O4 magnetic nanoparticles; Fourier transform infrared spectra; heterocyclic components; Hoechst staining; human breast cancer MCF‐7 cells; iron compounds; light scattering; magnetic particles; MCF10A nontumourigenic cells; MCF‐7 cell line; molecular biophysics; mortality rate; MTT assay; nanocompounds; nanofabrication; nanomagnetics; nanomedicine; nanoparticles; nuclear fragmentation; pyrazole derivatives; scanning electron microscopy; toxicology; tumour cells; tumours; X‐ray chemical analysis; X‐ray diffraction; Zeta potential
• ISSN: 1751-875X; 1751-8741; 1751-875X
• DOI: 10.1049/iet-nbt.2019.0199

Cancer is a major cause of death. Thus, the incidence and mortality rate of cancer is globally important. Regarding vast problems caused by chemotherapy drugs, efforts have progressed to find new anti‐cancer drugs. Pyrazole derivatives are known as components with anti‐cancer properties. In here, Fe3 O4 nanoparticles were first functionalized with (3‐chloropropyl) trimethoxysilane, then 2‐((pyrazol‐4‐yl) methylene) hydrazinecarbothioamide (P) was anchored on the surface of magnetic nanoparticles (PL). The synthesized nano‐compounds were characterized using Fourier transform infrared spectroscopy, X‐ray diffraction, scanning electron microscopy, Zeta potential, dynamic light scattering, and energy‐dispersive x‐ray spectrometry analyses. The cytotoxicity effect was evaluated using MTT assay, apoptosis test by Flow cytometry, cell cycle analysis, Caspase‐3 activity assay and Hoechst staining on MCF‐7 cell line. The high toxicity for tumor cells and low toxicity on normal cells (MCF10A) was considered as an important feature (selectivity index, 10.9). Based on results, the IC50 for P and PL compounds were 157.80 and 131.84 μM/ml respectively. Moreover, apoptosis inducing, nuclear fragmentation, Caspase 3 activity and induction of cell rest in sub‐G1 and S phases, were also observed. The inhibitory effect of PL was significantly higher than P, which could be due to the high penetrability of Fe3 O4 nanoparticles.