Substantial Improvement in Nontuberculous Mycobacterial Identification Using ASTA MicroIDSys Matrix-Assisted Laser Desorption/Ionization Time-of-Flight Mass Spectrometry with an Upgraded Database

Identifying using matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF MS) is challenging. We evaluated the performance of MALDI-TOF MS in identifying nontuberculous mycobacteria (NTM) using the ASTA MicroIDSys system (ASTA Inc., Suwon, Korea) with the MycoDB v1.95...

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Bibliographic Details
Published in:Annals of laboratory medicine 2022-05, Vol.42 (3), p.358-362
Main Authors: Song, Junhyup, Yoon, Shinyoung, In, Yongha, Kim, Daewon, Lee, Hyukmin, Yong, Dongeun, Lee, Kyoungwon
Format: Article
Language:English
Subjects:
Brief Communications
Culture Media
Humans
Lasers
Mycobacterium
Nontuberculous Mycobacteria - genetics
Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization
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Summary:Identifying using matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF MS) is challenging. We evaluated the performance of MALDI-TOF MS in identifying nontuberculous mycobacteria (NTM) using the ASTA MicroIDSys system (ASTA Inc., Suwon, Korea) with the MycoDB v1.95s and upgraded MycoDB v2.0-beta databases. We tested 124 NTM isolates collected from Ogawa medium at Severance Hospital, Seoul, Korea, between January and April 2019. MicroIDSys scores were categorized into three groups: ≥140, reliable identification; 130-139, ambiguous identification; and
ISSN:2234-3806
2234-3814
DOI:10.3343/alm.2022.42.3.358