Identification of Ehrlichia chaffeensis by nested PCR in ticks from southern China

A total of 717 ticks collected from southern China were examined by nested PCR for the presence of Ehrlichia chaffeensis. Sixteen (55. 2%) of 29 adult Amblyomma testudinarium ticks and 28 (11.7%) of 240 adult and at least 4.2% of 215 nymphal (pooled specimens) Haemaphysalis yeni ticks tested positiv...

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Bibliographic Details
Published in:Journal of clinical microbiology 2000-07, Vol.38 (7), p.2778-2780
Main Authors: CAO, W.-C, GAO, Y.-M, ZHANG, P.-H, ZHANG, X.-T, DAI, Q.-H, DUMLER, J. S, FANG, L.-Q, HONG YANG
Format: Article
Language:English
Subjects:
Amblyomma testudinarium
Animals
Arachnid Vectors - microbiology
Bacteriology
Biological and medical sciences
China
Chlamydiology and Rickettsiology
Cloning, Molecular
DNA, Bacterial - analysis
DNA, Bacterial - genetics
Ehrlichia chaffeensis
Ehrlichia chaffeensis - classification
Ehrlichia chaffeensis - genetics
Ehrlichia chaffeensis - isolation & purification
Epidemiology
Fundamental and applied biological sciences. Psychology
Haemaphysalis yeni
Ixodidae
Microbiology
Molecular Sequence Data
Nymph - microbiology
Polymerase Chain Reaction - methods
Sequence Analysis, DNA
Ticks - microbiology
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Summary:A total of 717 ticks collected from southern China were examined by nested PCR for the presence of Ehrlichia chaffeensis. Sixteen (55. 2%) of 29 adult Amblyomma testudinarium ticks and 28 (11.7%) of 240 adult and at least 4.2% of 215 nymphal (pooled specimens) Haemaphysalis yeni ticks tested positive. Four other species of ticks were negative. Selected positive amplicons were confirmed by DNA sequencing.
ISSN:0095-1137
1098-660X
DOI:10.1128/jcm.38.7.2778-2780.2000