Control of Specific/Nonspecific Protein Adsorption: Functionalization of Polyelectrolyte Multilayer Films as a Potential Coating for Biosensors

Control of nonspecific/specific protein adsorption is the main goal in the design of novel biomaterials, implants, drug delivery systems, and sensors. The specific functionalization of biomaterials can be achieved by proper surface modification. One of the important strategies is covering the materi...

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Bibliographic Details
Published in:Materials 2021-12, Vol.14 (24), p.7629
Main Authors: Kruk, Tomasz, Bzowska, Monika, Hinz, Alicja, Szuwarzyński, Michał, Szczepanowicz, Krzysztof
Format: Article
Language:English
Subjects:
Acids
Adsorption
Antibodies
Antigens
Biochemistry
Biocompatibility
Biomedical materials
Biosensors
Coatings
Drug carriers
Drug delivery systems
Ethylene glycol
Fibrinogen
Glutamic acid
Hydrogen bonds
Lysine
Monoclonal antibodies
Multilayers
Polyelectrolytes
Polyethylene glycol
Polymers
Protein adsorption
Proteins
Quartz crystals
Sensors
Serum albumin
Surface chemistry
Surgical implants
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Summary:Control of nonspecific/specific protein adsorption is the main goal in the design of novel biomaterials, implants, drug delivery systems, and sensors. The specific functionalization of biomaterials can be achieved by proper surface modification. One of the important strategies is covering the materials with functional coatings. Therefore, our work aimed to functionalize multilayer coating to control nonspecific/specific protein adsorption. The polyelectrolyte coating was formed using a layer-by-layer technique (LbL) with biocompatible polyelectrolytes poly-L-lysine hydrobromide (PLL) and poly-L-glutamic acid (PGA). Nonspecific protein adsorption was minimized/eliminated by pegylation of multilayer films, which was achieved by adsorption of pegylated polycations (PLL-g-PEG). The influence of poly (ethylene glycol) chain length on eliminating nonspecific protein adsorption was confirmed. Moreover, to achieve specific protein adsorption, the multilayer film was also functionalized by immobilization of antibodies via a streptavidin bridge. The functional coatings were tested, and the adsorption of the following proteins confirmed the ability to control nonspecific/specific adsorption: human serum albumin (HSA), fibrinogen (FIB), fetal bovine serum (FBS), carcinoembryonic antigen human (CEA) monitored by quartz crystal microbalance with dissipation (QCM-D). AFM imaging of unmodified and modified multilayer surfaces was also performed. Functional multilayer films are believed to have the potential as a novel platform for biotechnological applications, such as biosensors and nanocarriers for drug delivery systems.
ISSN:1996-1944
1996-1944
DOI:10.3390/ma14247629