Identification and Functional Analysis of Differentially Expressed Genes in Myzus persicae (Hemiptera: Aphididae) in Response to Trans-anethole

Abstract Plant essential oils, with high bioactivity and biodegradability, provide promising alternatives to synthetic pesticides for pest control. Trans-anethole is the major component of essential oil from star anise, Illicium verum Hook. The compound has a strong contact toxicity against the gree...

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Bibliographic Details
Published in:Journal of insect science (Tucson, Ariz.) Ariz.), 2022-01, Vol.22 (1)
Main Authors: Ding, Chao-Yang, Ma, Yu-Meng, Li, Bin, Wang, Yun, Zhao, Le, Peng, Jiang-Nan, Li, Mao-Ye, Liu, Su, Li, Shi-Guang
Format: Article
Language:English
Subjects:
Allylbenzene Derivatives - pharmacology
Animals
Anisoles - pharmacology
Aphididae
Aphids - drug effects
Aphids - genetics
Control
Environmental aspects
Gene Expression
Genetic aspects
Insect Proteins - genetics
Materia medica, Vegetable
Molecular Entomological Genetics
Oils, Volatile - pharmacology
Physiological aspects
Plant extracts
RNA sequencing
Star anise
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Summary:Abstract Plant essential oils, with high bioactivity and biodegradability, provide promising alternatives to synthetic pesticides for pest control. Trans-anethole is the major component of essential oil from star anise, Illicium verum Hook. The compound has a strong contact toxicity against the green peach aphid, Myzus persicae (Sulzer) (Hemiptera: Aphididae), which is a major insect pest of many vegetables and crops. However, little information is known about how M. persicae responds to trans-anethole at the molecular level. We conducted a comparative transcriptome analysis of M. persicae in response to a LD50 dose of trans-anethole. A total of 559 differentially expressed genes were detected in the treated individuals, with 318 genes up-regulated, and 241 genes down-regulated. Gene ontology (GO) analysis revealed that these genes were classified into different biological processes and pathways. We also found that genes encoding ATP-binding cassette (ABC) transporters, DnaJ, and cuticle proteins were dramatically up-regulated in response to trans-anethole. To study the function of these genes, we performed RNA interference (RNAi) analysis. Knockdown of an ABC transporter gene (ABCG4) and a DnaJ gene (DnaJC1) resulted in a significantly increased mortality rate in M. persicae following trans-anethole exposure, indicating the involvement of these two genes in the toxicity response to trans-anethole. The findings provide new insights into the mechanisms of M. persicae in coping with plant essential oils.
ISSN:1536-2442
1536-2442
DOI:10.1093/jisesa/ieab094