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Fast quantification of free amino acids in food by microfluidic voltage–assisted liquid desorption electrospray ionization–tandem mass spectrometry
A method based on microfluidic voltage–assisted liquid desorption electrospray ionization–tandem mass spectrometry (VAL-DESI-MS/MS) has been developed for fast quantification of free amino acids in food. Food extracts were transferred to the microfluidic platform and analyzed by liquid desorption ES...
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| Published in: | Analytical and bioanalytical chemistry 2020-03, Vol.412 (8), p.1947-1954 |
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| Main Authors: | , , , , , , , |
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| Language: | English |
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| cited_by | cdi_FETCH-LOGICAL-c578t-449e8a34495ebab516a7ee2b113e980d78e628ca2d304519178e2b8d581aa6513 |
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| cites | cdi_FETCH-LOGICAL-c578t-449e8a34495ebab516a7ee2b113e980d78e628ca2d304519178e2b8d581aa6513 |
| container_end_page | 1954 |
| container_issue | 8 |
| container_start_page | 1947 |
| container_title | Analytical and bioanalytical chemistry |
| container_volume | 412 |
| creator | Hu, Hankun Smith, Shila Li, Xiangtang Qian, Zhengming Su, Yaxia Lin, Manting Tu, Jiancheng Liu, Yi-Ming |
| description | A method based on microfluidic voltage–assisted liquid desorption electrospray ionization–tandem mass spectrometry (VAL-DESI-MS/MS) has been developed for fast quantification of free amino acids in food. Food extracts were transferred to the microfluidic platform and analyzed by liquid desorption ESI-MS/MS. Deuterated aspartic acid (i.e., 2,2,3-d
3
-Asp) was used as internal standard for analysis. The method had linear calibration curves with
r
2
values > 0.998. Limits of detection were at the level of sub μM for the amino acids tested, i.e., glutamic acid (Glu), arginine (Arg), tyrosine (Tyr), tryptophan (Trp), and phenylalanine (Phe). To validate the proposed method in food analysis, extracts of
Cordyceps
fungi were analyzed. Amino acid contents were found in the range from 0.63 mg/g (Tyr in
Cordyceps sinensis
) to 4.44 mg/g (Glu in
Cordyceps militaris
). Assay repeatability (RSD) was ≤ 5.2% for all the five amino acids measured in all the samples analyzed. Recovery was found in the range from 95.8 to 105.1% at two spiking concentrations of 0.250 mg/g and 1.00 mg/g. These results prove that the proposed microfluidic VAL-DESI-MS/MS method offers a quick and convenient means of quantifying free amino acids with accuracy and repeatability. Therefore, it may have potential in food analysis for nutritional and quality assessment purposes.
Graphical abstract |
| doi_str_mv | 10.1007/s00216-020-02450-w |
| format | article |
| fullrecord | <record><control><sourceid>gale_pubme</sourceid><recordid>TN_cdi_pubmedcentral_primary_oai_pubmedcentral_nih_gov_8717839</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><galeid>A615713244</galeid><sourcerecordid>A615713244</sourcerecordid><originalsourceid>FETCH-LOGICAL-c578t-449e8a34495ebab516a7ee2b113e980d78e628ca2d304519178e2b8d581aa6513</originalsourceid><addsrcrecordid>eNp9Uk1v1DAQjRCIlsIf4IAsceGS4o848V6QqooCUiUucLYce7K4Suxd22m1nPgPHPh__SWd7pYtRQhZ1ljj995oZl5VvWT0mFHavc2UctbWlFO8jaT11aPqkLVM1byV9PH-3fCD6lnOF5QyqVj7tDoQHEmCycPq15nJhaxnE4ofvDXFx0DiQIYEQMzkQyTGepeJD2SI0ZF-QyZvUxzG2TtvyWUci1nC9Y-fJmefCzgy-jX-EQc5ptVWEEawJcW8SmZDMOG_bwshqZjgYCITkklebVETlLR5Xj0ZzJjhxV08qr6evf9y-rE-__zh0-nJeW1lp0rdNAtQRmCQ0JtestZ0ALxnTMBCUdcpaLmyhjtBG8kWDBO8Vw7nYEwrmTiq3u10V3M_gbMQSjKjXiU_mbTR0Xj98Cf4b3oZL7XqUEssUODNnUCK6xly0ZPPFsbRBIhz1lxI1iiGAaGv_4JexDkFbA9RbdcKoZS6Ry3NCNqHIWJdeyuqT1omOyZ40yDq-B8oPDhNb2OAwWP-AYHvCLi7nBMM-x4Z1bd20js7aXSG3tpJXyHp1Z_T2VN--wcBYgfA1fqwhHTf0n9kbwCV0NsD</addsrcrecordid><sourcetype>Open Access Repository</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>2367633888</pqid></control><display><type>article</type><title>Fast quantification of free amino acids in food by microfluidic voltage–assisted liquid desorption electrospray ionization–tandem mass spectrometry</title><source>Springer Nature:Jisc Collections:Springer Nature Read and Publish 2023-2025: Springer Reading List</source><creator>Hu, Hankun ; Smith, Shila ; Li, Xiangtang ; Qian, Zhengming ; Su, Yaxia ; Lin, Manting ; Tu, Jiancheng ; Liu, Yi-Ming</creator><creatorcontrib>Hu, Hankun ; Smith, Shila ; Li, Xiangtang ; Qian, Zhengming ; Su, Yaxia ; Lin, Manting ; Tu, Jiancheng ; Liu, Yi-Ming</creatorcontrib><description>A method based on microfluidic voltage–assisted liquid desorption electrospray ionization–tandem mass spectrometry (VAL-DESI-MS/MS) has been developed for fast quantification of free amino acids in food. Food extracts were transferred to the microfluidic platform and analyzed by liquid desorption ESI-MS/MS. Deuterated aspartic acid (i.e., 2,2,3-d
3
-Asp) was used as internal standard for analysis. The method had linear calibration curves with
r
2
values > 0.998. Limits of detection were at the level of sub μM for the amino acids tested, i.e., glutamic acid (Glu), arginine (Arg), tyrosine (Tyr), tryptophan (Trp), and phenylalanine (Phe). To validate the proposed method in food analysis, extracts of
Cordyceps
fungi were analyzed. Amino acid contents were found in the range from 0.63 mg/g (Tyr in
Cordyceps sinensis
) to 4.44 mg/g (Glu in
Cordyceps militaris
). Assay repeatability (RSD) was ≤ 5.2% for all the five amino acids measured in all the samples analyzed. Recovery was found in the range from 95.8 to 105.1% at two spiking concentrations of 0.250 mg/g and 1.00 mg/g. These results prove that the proposed microfluidic VAL-DESI-MS/MS method offers a quick and convenient means of quantifying free amino acids with accuracy and repeatability. Therefore, it may have potential in food analysis for nutritional and quality assessment purposes.
Graphical abstract</description><identifier>ISSN: 1618-2642</identifier><identifier>EISSN: 1618-2650</identifier><identifier>DOI: 10.1007/s00216-020-02450-w</identifier><identifier>PMID: 32020315</identifier><language>eng</language><publisher>Berlin/Heidelberg: Springer Berlin Heidelberg</publisher><subject>Amino acids ; Amino Acids - analysis ; Analysis ; Analytical Chemistry ; Arginine ; Aspartic acid ; Biochemistry ; Characterization and Evaluation of Materials ; Chemistry ; Chemistry and Materials Science ; Desorption ; Deuteration ; Electric potential ; Electrospraying ; Food ; Food analysis ; Food Analysis - methods ; Food Science ; Fungi ; Glutamic acid ; Ionization ; Ions ; Laboratory Medicine ; Mass spectrometry ; Mass spectroscopy ; Microfluidics ; Monitoring/Environmental Analysis ; Phenylalanine ; Quality assessment ; Quality control ; Reproducibility ; Research Paper ; Scientific imaging ; Spectrometry, Mass, Electrospray Ionization - methods ; Spectroscopy ; Spectrum analysis ; Tandem Mass Spectrometry - methods ; Tryptophan ; Tyrosine ; Voltage</subject><ispartof>Analytical and bioanalytical chemistry, 2020-03, Vol.412 (8), p.1947-1954</ispartof><rights>Springer-Verlag GmbH Germany, part of Springer Nature 2020</rights><rights>COPYRIGHT 2020 Springer</rights><rights>Analytical and Bioanalytical Chemistry is a copyright of Springer, (2020). All Rights Reserved.</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c578t-449e8a34495ebab516a7ee2b113e980d78e628ca2d304519178e2b8d581aa6513</citedby><cites>FETCH-LOGICAL-c578t-449e8a34495ebab516a7ee2b113e980d78e628ca2d304519178e2b8d581aa6513</cites><orcidid>0000-0002-7760-9565</orcidid></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>230,314,777,781,882,27905,27906</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/32020315$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Hu, Hankun</creatorcontrib><creatorcontrib>Smith, Shila</creatorcontrib><creatorcontrib>Li, Xiangtang</creatorcontrib><creatorcontrib>Qian, Zhengming</creatorcontrib><creatorcontrib>Su, Yaxia</creatorcontrib><creatorcontrib>Lin, Manting</creatorcontrib><creatorcontrib>Tu, Jiancheng</creatorcontrib><creatorcontrib>Liu, Yi-Ming</creatorcontrib><title>Fast quantification of free amino acids in food by microfluidic voltage–assisted liquid desorption electrospray ionization–tandem mass spectrometry</title><title>Analytical and bioanalytical chemistry</title><addtitle>Anal Bioanal Chem</addtitle><addtitle>Anal Bioanal Chem</addtitle><description>A method based on microfluidic voltage–assisted liquid desorption electrospray ionization–tandem mass spectrometry (VAL-DESI-MS/MS) has been developed for fast quantification of free amino acids in food. Food extracts were transferred to the microfluidic platform and analyzed by liquid desorption ESI-MS/MS. Deuterated aspartic acid (i.e., 2,2,3-d
3
-Asp) was used as internal standard for analysis. The method had linear calibration curves with
r
2
values > 0.998. Limits of detection were at the level of sub μM for the amino acids tested, i.e., glutamic acid (Glu), arginine (Arg), tyrosine (Tyr), tryptophan (Trp), and phenylalanine (Phe). To validate the proposed method in food analysis, extracts of
Cordyceps
fungi were analyzed. Amino acid contents were found in the range from 0.63 mg/g (Tyr in
Cordyceps sinensis
) to 4.44 mg/g (Glu in
Cordyceps militaris
). Assay repeatability (RSD) was ≤ 5.2% for all the five amino acids measured in all the samples analyzed. Recovery was found in the range from 95.8 to 105.1% at two spiking concentrations of 0.250 mg/g and 1.00 mg/g. These results prove that the proposed microfluidic VAL-DESI-MS/MS method offers a quick and convenient means of quantifying free amino acids with accuracy and repeatability. Therefore, it may have potential in food analysis for nutritional and quality assessment purposes.
Graphical abstract</description><subject>Amino acids</subject><subject>Amino Acids - analysis</subject><subject>Analysis</subject><subject>Analytical Chemistry</subject><subject>Arginine</subject><subject>Aspartic acid</subject><subject>Biochemistry</subject><subject>Characterization and Evaluation of Materials</subject><subject>Chemistry</subject><subject>Chemistry and Materials Science</subject><subject>Desorption</subject><subject>Deuteration</subject><subject>Electric potential</subject><subject>Electrospraying</subject><subject>Food</subject><subject>Food analysis</subject><subject>Food Analysis - methods</subject><subject>Food Science</subject><subject>Fungi</subject><subject>Glutamic acid</subject><subject>Ionization</subject><subject>Ions</subject><subject>Laboratory Medicine</subject><subject>Mass spectrometry</subject><subject>Mass spectroscopy</subject><subject>Microfluidics</subject><subject>Monitoring/Environmental Analysis</subject><subject>Phenylalanine</subject><subject>Quality assessment</subject><subject>Quality control</subject><subject>Reproducibility</subject><subject>Research Paper</subject><subject>Scientific imaging</subject><subject>Spectrometry, Mass, Electrospray Ionization - methods</subject><subject>Spectroscopy</subject><subject>Spectrum analysis</subject><subject>Tandem Mass Spectrometry - methods</subject><subject>Tryptophan</subject><subject>Tyrosine</subject><subject>Voltage</subject><issn>1618-2642</issn><issn>1618-2650</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2020</creationdate><recordtype>article</recordtype><recordid>eNp9Uk1v1DAQjRCIlsIf4IAsceGS4o848V6QqooCUiUucLYce7K4Suxd22m1nPgPHPh__SWd7pYtRQhZ1ljj995oZl5VvWT0mFHavc2UctbWlFO8jaT11aPqkLVM1byV9PH-3fCD6lnOF5QyqVj7tDoQHEmCycPq15nJhaxnE4ofvDXFx0DiQIYEQMzkQyTGepeJD2SI0ZF-QyZvUxzG2TtvyWUci1nC9Y-fJmefCzgy-jX-EQc5ptVWEEawJcW8SmZDMOG_bwshqZjgYCITkklebVETlLR5Xj0ZzJjhxV08qr6evf9y-rE-__zh0-nJeW1lp0rdNAtQRmCQ0JtestZ0ALxnTMBCUdcpaLmyhjtBG8kWDBO8Vw7nYEwrmTiq3u10V3M_gbMQSjKjXiU_mbTR0Xj98Cf4b3oZL7XqUEssUODNnUCK6xly0ZPPFsbRBIhz1lxI1iiGAaGv_4JexDkFbA9RbdcKoZS6Ry3NCNqHIWJdeyuqT1omOyZ40yDq-B8oPDhNb2OAwWP-AYHvCLi7nBMM-x4Z1bd20js7aXSG3tpJXyHp1Z_T2VN--wcBYgfA1fqwhHTf0n9kbwCV0NsD</recordid><startdate>20200301</startdate><enddate>20200301</enddate><creator>Hu, Hankun</creator><creator>Smith, Shila</creator><creator>Li, Xiangtang</creator><creator>Qian, Zhengming</creator><creator>Su, Yaxia</creator><creator>Lin, Manting</creator><creator>Tu, Jiancheng</creator><creator>Liu, Yi-Ming</creator><general>Springer Berlin Heidelberg</general><general>Springer</general><general>Springer Nature B.V</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>3V.</scope><scope>7QF</scope><scope>7QO</scope><scope>7QQ</scope><scope>7SC</scope><scope>7SE</scope><scope>7SP</scope><scope>7SR</scope><scope>7TA</scope><scope>7TB</scope><scope>7U5</scope><scope>7U7</scope><scope>7X7</scope><scope>7XB</scope><scope>88E</scope><scope>8BQ</scope><scope>8FD</scope><scope>8FE</scope><scope>8FG</scope><scope>8FH</scope><scope>8FI</scope><scope>8FJ</scope><scope>8FK</scope><scope>ABJCF</scope><scope>ABUWG</scope><scope>AEUYN</scope><scope>AFKRA</scope><scope>AZQEC</scope><scope>BBNVY</scope><scope>BENPR</scope><scope>BGLVJ</scope><scope>BHPHI</scope><scope>C1K</scope><scope>CCPQU</scope><scope>D1I</scope><scope>DWQXO</scope><scope>F28</scope><scope>FR3</scope><scope>FYUFA</scope><scope>GHDGH</scope><scope>GNUQQ</scope><scope>H8D</scope><scope>H8G</scope><scope>HCIFZ</scope><scope>JG9</scope><scope>JQ2</scope><scope>K9.</scope><scope>KB.</scope><scope>KR7</scope><scope>L7M</scope><scope>LK8</scope><scope>L~C</scope><scope>L~D</scope><scope>M0S</scope><scope>M1P</scope><scope>M7P</scope><scope>P64</scope><scope>PDBOC</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>7X8</scope><scope>5PM</scope><orcidid>https://orcid.org/0000-0002-7760-9565</orcidid></search><sort><creationdate>20200301</creationdate><title>Fast quantification of free amino acids in food by microfluidic voltage–assisted liquid desorption electrospray ionization–tandem mass spectrometry</title><author>Hu, Hankun ; Smith, Shila ; Li, Xiangtang ; Qian, Zhengming ; Su, Yaxia ; Lin, Manting ; Tu, Jiancheng ; Liu, Yi-Ming</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c578t-449e8a34495ebab516a7ee2b113e980d78e628ca2d304519178e2b8d581aa6513</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2020</creationdate><topic>Amino acids</topic><topic>Amino Acids - analysis</topic><topic>Analysis</topic><topic>Analytical Chemistry</topic><topic>Arginine</topic><topic>Aspartic acid</topic><topic>Biochemistry</topic><topic>Characterization and Evaluation of Materials</topic><topic>Chemistry</topic><topic>Chemistry and Materials Science</topic><topic>Desorption</topic><topic>Deuteration</topic><topic>Electric potential</topic><topic>Electrospraying</topic><topic>Food</topic><topic>Food analysis</topic><topic>Food Analysis - methods</topic><topic>Food Science</topic><topic>Fungi</topic><topic>Glutamic acid</topic><topic>Ionization</topic><topic>Ions</topic><topic>Laboratory Medicine</topic><topic>Mass spectrometry</topic><topic>Mass spectroscopy</topic><topic>Microfluidics</topic><topic>Monitoring/Environmental Analysis</topic><topic>Phenylalanine</topic><topic>Quality assessment</topic><topic>Quality control</topic><topic>Reproducibility</topic><topic>Research Paper</topic><topic>Scientific imaging</topic><topic>Spectrometry, Mass, Electrospray Ionization - methods</topic><topic>Spectroscopy</topic><topic>Spectrum analysis</topic><topic>Tandem Mass Spectrometry - methods</topic><topic>Tryptophan</topic><topic>Tyrosine</topic><topic>Voltage</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Hu, Hankun</creatorcontrib><creatorcontrib>Smith, Shila</creatorcontrib><creatorcontrib>Li, Xiangtang</creatorcontrib><creatorcontrib>Qian, Zhengming</creatorcontrib><creatorcontrib>Su, Yaxia</creatorcontrib><creatorcontrib>Lin, Manting</creatorcontrib><creatorcontrib>Tu, Jiancheng</creatorcontrib><creatorcontrib>Liu, Yi-Ming</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>ProQuest Central (Corporate)</collection><collection>Aluminium Industry Abstracts</collection><collection>Biotechnology Research Abstracts</collection><collection>Ceramic Abstracts</collection><collection>Computer and Information Systems Abstracts</collection><collection>Corrosion Abstracts</collection><collection>Electronics & Communications Abstracts</collection><collection>Engineered Materials Abstracts</collection><collection>Materials Business File</collection><collection>Mechanical & Transportation Engineering Abstracts</collection><collection>Solid State and Superconductivity Abstracts</collection><collection>Toxicology Abstracts</collection><collection>Health & Medical Collection</collection><collection>ProQuest Central (purchase pre-March 2016)</collection><collection>Medical Database (Alumni Edition)</collection><collection>METADEX</collection><collection>Technology Research Database</collection><collection>ProQuest SciTech Collection</collection><collection>ProQuest Technology Collection</collection><collection>ProQuest Natural Science Collection</collection><collection>Hospital Premium Collection</collection><collection>Hospital Premium Collection (Alumni Edition)</collection><collection>ProQuest Central (Alumni) (purchase pre-March 2016)</collection><collection>Materials Science & Engineering Collection</collection><collection>ProQuest Central (Alumni Edition)</collection><collection>ProQuest One Sustainability</collection><collection>ProQuest Central UK/Ireland</collection><collection>ProQuest Central Essentials</collection><collection>Biological Science Collection</collection><collection>ProQuest Central</collection><collection>Technology Collection</collection><collection>Natural Science Collection</collection><collection>Environmental Sciences and Pollution Management</collection><collection>ProQuest One Community College</collection><collection>ProQuest Materials Science Collection</collection><collection>ProQuest Central Korea</collection><collection>ANTE: Abstracts in New Technology & Engineering</collection><collection>Engineering Research Database</collection><collection>Health Research Premium Collection</collection><collection>Health Research Premium Collection (Alumni)</collection><collection>ProQuest Central Student</collection><collection>Aerospace Database</collection><collection>Copper Technical Reference Library</collection><collection>SciTech Premium Collection</collection><collection>Materials Research Database</collection><collection>ProQuest Computer Science Collection</collection><collection>ProQuest Health & Medical Complete (Alumni)</collection><collection>Materials Science Database</collection><collection>Civil Engineering Abstracts</collection><collection>Advanced Technologies Database with Aerospace</collection><collection>ProQuest Biological Science Collection</collection><collection>Computer and Information Systems Abstracts Academic</collection><collection>Computer and Information Systems Abstracts Professional</collection><collection>Health & Medical Collection (Alumni Edition)</collection><collection>Medical Database</collection><collection>Biological Science Database</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>Materials Science Collection</collection><collection>ProQuest One Academic Eastern Edition (DO NOT USE)</collection><collection>ProQuest One Academic</collection><collection>ProQuest One Academic UKI Edition</collection><collection>MEDLINE - Academic</collection><collection>PubMed Central (Full Participant titles)</collection><jtitle>Analytical and bioanalytical chemistry</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Hu, Hankun</au><au>Smith, Shila</au><au>Li, Xiangtang</au><au>Qian, Zhengming</au><au>Su, Yaxia</au><au>Lin, Manting</au><au>Tu, Jiancheng</au><au>Liu, Yi-Ming</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Fast quantification of free amino acids in food by microfluidic voltage–assisted liquid desorption electrospray ionization–tandem mass spectrometry</atitle><jtitle>Analytical and bioanalytical chemistry</jtitle><stitle>Anal Bioanal Chem</stitle><addtitle>Anal Bioanal Chem</addtitle><date>2020-03-01</date><risdate>2020</risdate><volume>412</volume><issue>8</issue><spage>1947</spage><epage>1954</epage><pages>1947-1954</pages><issn>1618-2642</issn><eissn>1618-2650</eissn><abstract>A method based on microfluidic voltage–assisted liquid desorption electrospray ionization–tandem mass spectrometry (VAL-DESI-MS/MS) has been developed for fast quantification of free amino acids in food. Food extracts were transferred to the microfluidic platform and analyzed by liquid desorption ESI-MS/MS. Deuterated aspartic acid (i.e., 2,2,3-d
3
-Asp) was used as internal standard for analysis. The method had linear calibration curves with
r
2
values > 0.998. Limits of detection were at the level of sub μM for the amino acids tested, i.e., glutamic acid (Glu), arginine (Arg), tyrosine (Tyr), tryptophan (Trp), and phenylalanine (Phe). To validate the proposed method in food analysis, extracts of
Cordyceps
fungi were analyzed. Amino acid contents were found in the range from 0.63 mg/g (Tyr in
Cordyceps sinensis
) to 4.44 mg/g (Glu in
Cordyceps militaris
). Assay repeatability (RSD) was ≤ 5.2% for all the five amino acids measured in all the samples analyzed. Recovery was found in the range from 95.8 to 105.1% at two spiking concentrations of 0.250 mg/g and 1.00 mg/g. These results prove that the proposed microfluidic VAL-DESI-MS/MS method offers a quick and convenient means of quantifying free amino acids with accuracy and repeatability. Therefore, it may have potential in food analysis for nutritional and quality assessment purposes.
Graphical abstract</abstract><cop>Berlin/Heidelberg</cop><pub>Springer Berlin Heidelberg</pub><pmid>32020315</pmid><doi>10.1007/s00216-020-02450-w</doi><tpages>8</tpages><orcidid>https://orcid.org/0000-0002-7760-9565</orcidid><oa>free_for_read</oa></addata></record> |
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| source | Springer Nature:Jisc Collections:Springer Nature Read and Publish 2023-2025: Springer Reading List |
| subjects | Amino acids Amino Acids - analysis Analysis Analytical Chemistry Arginine Aspartic acid Biochemistry Characterization and Evaluation of Materials Chemistry Chemistry and Materials Science Desorption Deuteration Electric potential Electrospraying Food Food analysis Food Analysis - methods Food Science Fungi Glutamic acid Ionization Ions Laboratory Medicine Mass spectrometry Mass spectroscopy Microfluidics Monitoring/Environmental Analysis Phenylalanine Quality assessment Quality control Reproducibility Research Paper Scientific imaging Spectrometry, Mass, Electrospray Ionization - methods Spectroscopy Spectrum analysis Tandem Mass Spectrometry - methods Tryptophan Tyrosine Voltage |
| title | Fast quantification of free amino acids in food by microfluidic voltage–assisted liquid desorption electrospray ionization–tandem mass spectrometry |
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