Proteomic and Immunological Identification of Diagnostic Antigens from Spirometra erinaceieuropaei Plerocercoid

Human sparganosis is a food-borne parasitic disease caused by the plerocercoids of Spirometra species. Clinical diagnosis of sparganosis is crucial for effective treatment, thus it is important to identify sensitive and specific antigens of plerocercoids. The aim of the current study was to identify...

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Bibliographic Details
Published in:Korean journal of parasitology 2021, Hosts and Diseases, 59(6), , pp.615-623
Main Authors: Lu, Yan, Sun, Jia-Hui, Lu, Li-Li, Chen, Jia-Xu, Song, Peng, Ai, Lin, Cai, Yu-Chun, Li, Lan-Hua, Chen, Shao-Hong
Format: Article
Language:English
Subjects:
Animals
Electrophoresis, Gel, Two-Dimensional
Humans
Immunoblotting
Original
Proteomics
Sparganosis - diagnosis
Spirometra
예방의학
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Summary:Human sparganosis is a food-borne parasitic disease caused by the plerocercoids of Spirometra species. Clinical diagnosis of sparganosis is crucial for effective treatment, thus it is important to identify sensitive and specific antigens of plerocercoids. The aim of the current study was to identify and characterize the immunogenic proteins of Spirometra erinaceieuropaei plerocercoids that were recognized by patient sera. Crude soluble extract of the plerocercoids were separated using 2-dimensional gel electrophoresis coupled with immunoblot and mass spectrometry analysis. Based on immunoblotting patterns and mass spectrometry results, 8 antigenic proteins were identified from the plerocercoid. Among the proteins, cysteine protease protein might be developed as an antigen for diagnosis of sparganosis.
ISSN:0023-4001
2982-5164
1738-0006
2982-6799
DOI:10.3347/kjp.2021.59.6.615