LOXL1‐AS1 communicating with TIAR modulates vasculogenic mimicry in glioma via regulation of the miR‐374b‐5p/MMP14 axis

At present, growing evidence indicates that long non‐coding RNAs (lncRNAs) participate in the progression of glioma. The function of LOXL1‐AS1 in vasculogenic mimicry (VM) in glioma remains unclear. First, the expressions of TIAR, the lncRNA LOXL1‐AS1, miR‐374b‐5p and MMP14 were examined by qRT‐PCR...

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Published in:Journal of cellular and molecular medicine 2022-01, Vol.26 (2), p.475-490
Main Authors: Yi, Bolong, Li, Hao, Cai, Heng, Lou, Xin, Yu, Mingjun, Li, Zhen
Format: Article
Language:English
Subjects:
Amino Acid Oxidoreductases - metabolism
Angiogenesis
Animals
Antibodies
Behavior
CD34 antigen
Cell Line, Tumor
Cell lines
Cell migration
Cell Movement - genetics
Cell proliferation
Cell Proliferation - genetics
Colorectal cancer
Gene Expression Regulation, Neoplastic
Glioma
Glioma - genetics
Glioma - pathology
Glioma cells
Humans
Liver cancer
LOXL1‐AS1
Lung cancer
Matrix Metalloproteinase 14 - genetics
Matrix Metalloproteinase 14 - metabolism
Medical prognosis
Membranes
Metastasis
Mice
MicroRNAs
MicroRNAs - genetics
MicroRNAs - metabolism
Mimicry
miRNA
miR‐374b‐5p
MMP14
Nervous system
Non-coding RNA
Original
Proteins
RNA, Long Noncoding - genetics
RNA-Binding Proteins - metabolism
TIAR
Tumors
vasculogenic mimicry
Xenografts
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description At present, growing evidence indicates that long non‐coding RNAs (lncRNAs) participate in the progression of glioma. The function of LOXL1‐AS1 in vasculogenic mimicry (VM) in glioma remains unclear. First, the expressions of TIAR, the lncRNA LOXL1‐AS1, miR‐374b‐5p and MMP14 were examined by qRT‐PCR and Western blot in both, glioma tissues and glioma cell lines. Proliferation, migration, invasion and tube formation assays were conducted to evaluate the roles of TIAR, LOXL1‐AS1, miR‐374b‐5p and MMP14 in malignant cellular behaviours in glioma cells. A nude mouse xenograft model and dual staining for CD34 and PAS were used to assess whether VM was affected by TIAR, LOXL1‐AS1 or miR‐374b‐5p in vivo. In this study, low levels of TIAR and high levels of LOXL1‐AS1 were found in glioma cells and tissues. TIAR downregulated the expression of LOXL1‐AS1 by destabilizing it. LOXL1‐AS1 acted like a miRNA sponge towards miR‐374b‐5p so that downregulation of the former greatly inhibited cell proliferation, migration, invasion and VM. Additionally, miR‐374b‐5p overexpression repressed malignant biological behaviours and VM in glioma by modifying MMP14. In summary, we demonstrated that TIAR combined with LOXL1‐AS1 modulates VM in glioma via the miR‐374b‐5p/MMP14 axis, revealing novel targets for glioma therapy.
doi_str_mv 10.1111/jcmm.17106
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The function of LOXL1‐AS1 in vasculogenic mimicry (VM) in glioma remains unclear. First, the expressions of TIAR, the lncRNA LOXL1‐AS1, miR‐374b‐5p and MMP14 were examined by qRT‐PCR and Western blot in both, glioma tissues and glioma cell lines. Proliferation, migration, invasion and tube formation assays were conducted to evaluate the roles of TIAR, LOXL1‐AS1, miR‐374b‐5p and MMP14 in malignant cellular behaviours in glioma cells. A nude mouse xenograft model and dual staining for CD34 and PAS were used to assess whether VM was affected by TIAR, LOXL1‐AS1 or miR‐374b‐5p in vivo. In this study, low levels of TIAR and high levels of LOXL1‐AS1 were found in glioma cells and tissues. TIAR downregulated the expression of LOXL1‐AS1 by destabilizing it. LOXL1‐AS1 acted like a miRNA sponge towards miR‐374b‐5p so that downregulation of the former greatly inhibited cell proliferation, migration, invasion and VM. Additionally, miR‐374b‐5p overexpression repressed malignant biological behaviours and VM in glioma by modifying MMP14. In summary, we demonstrated that TIAR combined with LOXL1‐AS1 modulates VM in glioma via the miR‐374b‐5p/MMP14 axis, revealing novel targets for glioma therapy.</description><identifier>ISSN: 1582-1838</identifier><identifier>EISSN: 1582-4934</identifier><identifier>DOI: 10.1111/jcmm.17106</identifier><identifier>PMID: 34890108</identifier><language>eng</language><publisher>England: John Wiley &amp; Sons, Inc</publisher><subject>Amino Acid Oxidoreductases - metabolism ; Angiogenesis ; Animals ; Antibodies ; Behavior ; CD34 antigen ; Cell Line, Tumor ; Cell lines ; Cell migration ; Cell Movement - genetics ; Cell proliferation ; Cell Proliferation - genetics ; Colorectal cancer ; Gene Expression Regulation, Neoplastic ; Glioma ; Glioma - genetics ; Glioma - pathology ; Glioma cells ; Humans ; Liver cancer ; LOXL1‐AS1 ; Lung cancer ; Matrix Metalloproteinase 14 - genetics ; Matrix Metalloproteinase 14 - metabolism ; Medical prognosis ; Membranes ; Metastasis ; Mice ; MicroRNAs ; MicroRNAs - genetics ; MicroRNAs - metabolism ; Mimicry ; miRNA ; miR‐374b‐5p ; MMP14 ; Nervous system ; Non-coding RNA ; Original ; Proteins ; RNA, Long Noncoding - genetics ; RNA-Binding Proteins - metabolism ; TIAR ; Tumors ; vasculogenic mimicry ; Xenografts</subject><ispartof>Journal of cellular and molecular medicine, 2022-01, Vol.26 (2), p.475-490</ispartof><rights>2021 The Authors. published by Foundation for Cellular and Molecular Medicine and John Wiley &amp; Sons Ltd.</rights><rights>2021 The Authors. Journal of Cellular and Molecular Medicine published by Foundation for Cellular and Molecular Medicine and John Wiley &amp; Sons Ltd.</rights><rights>2022. This work is published under http://creativecommons.org/licenses/by/4.0/ (the “License”). 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The function of LOXL1‐AS1 in vasculogenic mimicry (VM) in glioma remains unclear. First, the expressions of TIAR, the lncRNA LOXL1‐AS1, miR‐374b‐5p and MMP14 were examined by qRT‐PCR and Western blot in both, glioma tissues and glioma cell lines. Proliferation, migration, invasion and tube formation assays were conducted to evaluate the roles of TIAR, LOXL1‐AS1, miR‐374b‐5p and MMP14 in malignant cellular behaviours in glioma cells. A nude mouse xenograft model and dual staining for CD34 and PAS were used to assess whether VM was affected by TIAR, LOXL1‐AS1 or miR‐374b‐5p in vivo. In this study, low levels of TIAR and high levels of LOXL1‐AS1 were found in glioma cells and tissues. TIAR downregulated the expression of LOXL1‐AS1 by destabilizing it. LOXL1‐AS1 acted like a miRNA sponge towards miR‐374b‐5p so that downregulation of the former greatly inhibited cell proliferation, migration, invasion and VM. 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subjects Amino Acid Oxidoreductases - metabolism
Angiogenesis
Animals
Antibodies
Behavior
CD34 antigen
Cell Line, Tumor
Cell lines
Cell migration
Cell Movement - genetics
Cell proliferation
Cell Proliferation - genetics
Colorectal cancer
Gene Expression Regulation, Neoplastic
Glioma
Glioma - genetics
Glioma - pathology
Glioma cells
Humans
Liver cancer
LOXL1‐AS1
Lung cancer
Matrix Metalloproteinase 14 - genetics
Matrix Metalloproteinase 14 - metabolism
Medical prognosis
Membranes
Metastasis
Mice
MicroRNAs
MicroRNAs - genetics
MicroRNAs - metabolism
Mimicry
miRNA
miR‐374b‐5p
MMP14
Nervous system
Non-coding RNA
Original
Proteins
RNA, Long Noncoding - genetics
RNA-Binding Proteins - metabolism
TIAR
Tumors
vasculogenic mimicry
Xenografts
title LOXL1‐AS1 communicating with TIAR modulates vasculogenic mimicry in glioma via regulation of the miR‐374b‐5p/MMP14 axis
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