Comparison of Danish isolates of Salmonella enterica serovar Enteritidis PT9a and PT11 from hedgehogs (Erinaceus europaeus) and humans by plasmid profiling and pulsed-field gel electrophoresis

During the years 1994 to 1998, 10 strains of Salmonella enterica serovar Enteritidis phage type 11 (PT11) and 6 PT9a strains were isolated from Danish hedgehogs, together with 7 strains that did not yield phage susceptibility patterns conforming with any known phage type (routine dilution no conform...

Full description

Saved in:
Bibliographic Details
Published in:Journal of clinical microbiology 2000-10, Vol.38 (10), p.3631-3635
Main Authors: NAUERBY, B, PEDERSEN, K, DIETZ, H. H, MADSEN, M
Format: Article
Language:English
Subjects:
Animals
Bacteriology
Bacteriophage Typing
Biological and medical sciences
Denmark
Deoxyribonuclease HindIII
Electrophoresis, Gel, Pulsed-Field - methods
Epidemiology
Erinaceus europaeus
Fundamental and applied biological sciences. Psychology
Geography
Hedgehogs - microbiology
Humans
Microbiology
Plasmids
Polymorphism, Restriction Fragment Length
Salmonella enterica enteritidis
Salmonella enteritidis - classification
Salmonella enteritidis - genetics
Salmonella enteritidis - isolation & purification
Citations: Items that this one cites
Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:During the years 1994 to 1998, 10 strains of Salmonella enterica serovar Enteritidis phage type 11 (PT11) and 6 PT9a strains were isolated from Danish hedgehogs, together with 7 strains that did not yield phage susceptibility patterns conforming with any known phage type (routine dilution no conformity [RDNC]). From 1995 to 1998, five Danish patients were reported infected with serovar Enteritidis PT11 and two with PT9a. All serovar Enteritidis PT11, PT9a, and RDNC isolates from hedgehogs and humans were analyzed by pulsed-field gel electrophoresis (PFGE), plasmid profiling, and restriction fragment length polymorphism (RFLP) of plasmids. By use of S1 nuclease and HindIII, the PT11 and PT9a isolates had identical plasmid profiles and RFLP patterns, which differed from the RDNC profiles. The PFGE profiles were identical for all serovar Enteritidis PT11 and PT9a strains from hedgehogs, four of five human strains of serovar Enteritidis PT11, and two human strains of serovar Enteritidis PT9a, irrespective of restriction enzyme, whereas the last human strain deviated slightly when NotI was used but not when XbaI or SpeI was used. The results indicate that serovar Enteritidis PT9a and PT11 are closely related and that PT11 and PT9a from Danish hedgehogs and humans belong to the same clonal lineage.
ISSN:0095-1137
1098-660X
DOI:10.1128/JCM.38.10.3631-3635.2000