Development and Optimization of Acriflavine-Loaded Polycaprolactone Nanoparticles Using Box-Behnken Design for Burn Wound Healing Applications

Nanoparticles are used increasingly for the treatment of different disorders, including burn wounds of the skin, due to their important role in wound healing. In this study, acriflavine-loaded poly (ε-caprolactone) nanoparticles (ACR-PCL-NPs) were prepared using a double-emulsion solvent evaporation...

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Bibliographic Details
Published in:Polymers 2021-12, Vol.14 (1), p.101
Main Authors: Nawaz, Touseef, Iqbal, Muhammad, Khan, Barkat Ali, Nawaz, Asif, Hussain, Talib, Hosny, Khaled M, Abualsunun, Walaa A, Rizg, Waleed Y
Format: Article
Language:English
Subjects:
Acriflavine
Biomedical materials
Biopolymers
Dependent variables
Drugs
Efficiency
Encapsulation
Independent variables
Laboratories
Morphology
Nanoparticles
Optimization
Particle size
Polycaprolactone
Polydispersity
Polyesters
Polyvinyl alcohol
Shape optimization
Solvents
Wound healing
Zeta potential
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Summary:Nanoparticles are used increasingly for the treatment of different disorders, including burn wounds of the skin, due to their important role in wound healing. In this study, acriflavine-loaded poly (ε-caprolactone) nanoparticles (ACR-PCL-NPs) were prepared using a double-emulsion solvent evaporation method. All the formulations were prepared and optimized by using a Box-Behnken design. Formulations were evaluated for the effect of independent variables, i.e., poly (ε-caprolactone) (PCL) amount (X1), stirring speed of external phase (X2), and polyvinyl alcohol (PVA) concentration (X3), on the formulation-dependent variables (particle size, polydispersity index (PDI), and encapsulation efficiency) of ACR-PCL-NPs. The zeta potential, PDI, particle size, and encapsulation efficiency of optimized ACR-PCL-NPs were found to be -3.98 ± 1.58 mV, 0.270 ± 0.19, 469.2 ± 5.6 nm, and 71.9 ± 5.32%, respectively. The independent variables were found to be in excellent correlation with the dependent variables. The release of acriflavine from optimized ACR-PCL-NPs was in biphasic style with the initial burst release, followed by a slow release for up to 24 h of the in vitro study. Morphological studies of optimized ACR-PCL-NPs revealed the smooth surfaces and spherical shapes of the particles. Thermal and FTIR analyses revealed the drug-polymer compatibility of ACR-PCL-NPs. The drug-treated group showed significant re-epithelialization, as compared to the controlled group.
ISSN:2073-4360
2073-4360
DOI:10.3390/polym14010101