Cancer-Associated Stromal Cells Promote the Contribution of MMP2-Positive Bone Marrow-Derived Cells to Oral Squamous Cell Carcinoma Invasion

Tumor stromal components contribute to tumor development and invasion. However, the role of stromal cells in the contribution of bone marrow-derived cells (BMDCs) in oral squamous cell carcinoma (OSCC) invasion is unclear. In the present study, we created two different invasive OSCC patient-derived...

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Bibliographic Details
Published in:Cancers 2021-12, Vol.14 (1), p.137
Main Authors: Oo, May Wathone, Kawai, Hotaka, Takabatake, Kiyofumi, Shan, Qiusheng, Eain, Htoo Shwe, Sukegawa, Shintaro, Nakano, Keisuke, Nagatsuka, Hitoshi
Format: Article
Language:English
Subjects:
Antibiotics
Antibodies
Antigens
Bioinformatics
Bone marrow
Bone marrow transplantation
Bone tumors
Cancer
Cell culture
Chemotherapy
Extracellular matrix
Gelatinase A
Green fluorescent protein
IL-1β
Immunohistochemistry
Interleukin 1
Interleukin 6
Invasiveness
Laboratories
Matrix metalloproteinase
Metalloproteinase
Metastasis
mRNA
Oral carcinoma
Oral squamous cell carcinoma
Patients
Proteins
Stromal cells
Xenografts
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Summary:Tumor stromal components contribute to tumor development and invasion. However, the role of stromal cells in the contribution of bone marrow-derived cells (BMDCs) in oral squamous cell carcinoma (OSCC) invasion is unclear. In the present study, we created two different invasive OSCC patient-derived stroma xenografts (PDSXs) and analyzed and compared the effects of stromal cells on the relation of BMDCs and tumor invasion. We isolated stromal cells from two OSCC patients: less invasive verrucous OSCC (VSCC) and highly invasive conventional OSCC (SCC) and co-xenografted with the OSCC cell line (HSC-2) on green fluorescent protein (GFP)-positive bone marrow (BM) cells transplanted mice. We traced the GFP-positive BM cells by immunohistochemistry (IHC) and detected matrix metalloproteinase 2 (MMP2) expression on BM cells by double fluorescent IHC. The results indicated that the SCC-PDSX promotes MMP2-positive BMDCs recruitment to the invasive front line of the tumor. Furthermore, microarray analysis revealed that the expressions of interleukin 6; mRNA and interleukin 1 beta; mRNA were higher in SCC stromal cells than in VSCC stromal cells. Thus, our study first reports that IL-6 and IL1B might be the potential stromal factors promoting the contribution of MMP2-positive BMDCs to OSCC invasion.
ISSN:2072-6694
2072-6694
DOI:10.3390/cancers14010137