Making neurons, made easy: The use of Neurogenin-2 in neuronal differentiation

Directed neuronal differentiation of human pluripotent stem cells (hPSCs), neural progenitors, or fibroblasts using transcription factors has allowed for the rapid and highly reproducible differentiation of mature and functional neurons. Exogenous expression of the transcription factor Neurogenin-2...

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Bibliographic Details
Published in:Stem cell reports 2022-01, Vol.17 (1), p.14-34
Main Authors: Hulme, Amy J., Maksour, Simon, St-Clair Glover, Mitchell, Miellet, Sara, Dottori, Mirella
Format: Article
Language:English
Subjects:
induced neurons
neural differentiation
neural progenitors
Neurogenin-2
NGN2
pluripotent stem cells
Review
transcription factor
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Summary:Directed neuronal differentiation of human pluripotent stem cells (hPSCs), neural progenitors, or fibroblasts using transcription factors has allowed for the rapid and highly reproducible differentiation of mature and functional neurons. Exogenous expression of the transcription factor Neurogenin-2 (NGN2) has been widely used to generate different populations of neurons, which have been used in neurodevelopment studies, disease modeling, drug screening, and neuronal replacement therapies. Could NGN2 be a “one-glove-fits-all” approach for neuronal differentiations? This review summarizes the cellular roles of NGN2 and describes the applications and limitations of using NGN2 for the rapid and directed differentiation of neurons. [Display omitted] Forced expression of the transcription factor NGN2 has been widely used to directly convert hPCSs, neural progenitors, or fibroblasts to functionally mature neurons of the central and peripheral nervous system (PNS). The article by Dottori and colleagues summarizes the cellular roles of NGN2 and describes the applications and limitations of using NGN2 for directed differentiation of neurons.
ISSN:2213-6711
2213-6711
DOI:10.1016/j.stemcr.2021.11.015