Development of effective biotransformation process for benzohydroxamic acid production using Bacillus smithii IIIMB2907

The present study entails the usefulness of thermophilic amidase-producing bacterium in the biotransformation of benzamide to benzohydroxamic acid (BHA). A bacterium  Bacillus smithii  IIIMB2907 was isolated from a soil sample collected from hot springs of Manikaran, Himachal Pradesh, India. The who...

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Bibliographic Details
Published in:3 Biotech 2022-02, Vol.12 (2), p.44-44, Article 44
Main Authors: Sharma, Hitesh, Singh, Rahul Vikram, Ganjoo, Ananta, Kumar, Amit, Singh, Ravail, Babu, Vikash
Format: Article
Language:English
Subjects:
Acid production
Acyltransferase
Agriculture
Amidase
Amides
Bacillus smithii
Benzamide
Bioinformatics
Biomaterials
Biotechnology
Biotransformation
Cancer Research
Caprolactam
Chemistry
Chemistry and Materials Science
Enzymes
Hot springs
Original
Original Article
Stem Cells
Substrate specificity
Substrates
Thermophilic bacteria
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Summary:The present study entails the usefulness of thermophilic amidase-producing bacterium in the biotransformation of benzamide to benzohydroxamic acid (BHA). A bacterium  Bacillus smithii  IIIMB2907 was isolated from a soil sample collected from hot springs of Manikaran, Himachal Pradesh, India. The whole cells of the bacterium displayed versatile substrate specificity by exhibiting significant activity with a diverse range of amides. In addition, amidase from thermophilic bacterium was induced by adding Ɛ-caprolactam in the mineral base media. The optimum temperature and pH of acyltransferase activity of amidase enzyme were found to be 50 °C and 7.0, respectively. Interestingly, half-life ( t 1/2 ) of this enzyme was 17.37 h at 50 °C. Bench-scale production and purification of BHA was carried out at optimized conditions which resulted in the recovery of 64% BHA with a purity of 96%. Owing to this, the reported process in the present study can be considered of immense industrial significance for the production of BHA.
ISSN:2190-572X
2190-5738
DOI:10.1007/s13205-022-03109-2