Loading…
Development of effective biotransformation process for benzohydroxamic acid production using Bacillus smithii IIIMB2907
The present study entails the usefulness of thermophilic amidase-producing bacterium in the biotransformation of benzamide to benzohydroxamic acid (BHA). A bacterium Bacillus smithii IIIMB2907 was isolated from a soil sample collected from hot springs of Manikaran, Himachal Pradesh, India. The who...
Saved in:
| Published in: | 3 Biotech 2022-02, Vol.12 (2), p.44-44, Article 44 |
|---|---|
| Main Authors: | , , , , , |
| Format: | Article |
| Language: | English |
| Subjects: | |
| Citations: | Items that this one cites Items that cite this one |
| Online Access: | Get full text |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| cited_by | cdi_FETCH-LOGICAL-c474t-ce0a188963cf9c4e5b766ca2346dd376236001070c0ad913f4452103ac517ffb3 |
|---|---|
| cites | cdi_FETCH-LOGICAL-c474t-ce0a188963cf9c4e5b766ca2346dd376236001070c0ad913f4452103ac517ffb3 |
| container_end_page | 44 |
| container_issue | 2 |
| container_start_page | 44 |
| container_title | 3 Biotech |
| container_volume | 12 |
| creator | Sharma, Hitesh Singh, Rahul Vikram Ganjoo, Ananta Kumar, Amit Singh, Ravail Babu, Vikash |
| description | The present study entails the usefulness of thermophilic amidase-producing bacterium in the biotransformation of benzamide to benzohydroxamic acid (BHA). A bacterium
Bacillus smithii
IIIMB2907 was isolated from a soil sample collected from hot springs of Manikaran, Himachal Pradesh, India. The whole cells of the bacterium displayed versatile substrate specificity by exhibiting significant activity with a diverse range of amides. In addition, amidase from thermophilic bacterium was induced by adding Ɛ-caprolactam in the mineral base media. The optimum temperature and pH of acyltransferase activity of amidase enzyme were found to be 50 °C and 7.0, respectively. Interestingly, half-life (
t
1/2
) of this enzyme was 17.37 h at 50 °C. Bench-scale production and purification of BHA was carried out at optimized conditions which resulted in the recovery of 64% BHA with a purity of 96%. Owing to this, the reported process in the present study can be considered of immense industrial significance for the production of BHA. |
| doi_str_mv | 10.1007/s13205-022-03109-2 |
| format | article |
| fullrecord | <record><control><sourceid>proquest_pubme</sourceid><recordid>TN_cdi_pubmedcentral_primary_oai_pubmedcentral_nih_gov_8761202</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><sourcerecordid>2624189906</sourcerecordid><originalsourceid>FETCH-LOGICAL-c474t-ce0a188963cf9c4e5b766ca2346dd376236001070c0ad913f4452103ac517ffb3</originalsourceid><addsrcrecordid>eNp9kUuPFCEUhYnROJNx_oALQ-LGTekFCig2Js746mSMG03cEYqCbiZV0EJV6_jrpafH9rGQDeTej3PvyUHoMYHnBEC-KIRR4A1Q2gAjoBp6D51SoqDhknX3j2_65QSdl3IN9XDCFYGH6IRxUIIDOUXfXrudG9N2cnHGyWPnvbNz2DnchzRnE4tPeTJzSBFvc7KuFFwruHfxR9rcDDl9N1Ow2Ngw7IFhsbfsUkJc44taHsel4DKFeRMCXq1WHy6oAvkIPfBmLO787j5Dn9---XT5vrn6-G51-eqqsa1s58Y6MKTrlGDWK9s63kshrKGsFcPApKBMABCQYMEMijDftpwSYMZyIr3v2Rl6edDdLv3kBlttZjPqbQ6TyTc6maD_7sSw0eu0050UhAKtAs_uBHL6urgy6ykU68bRRJeWoqmgLemUAlHRp_-g12nJsdqrFFEdZ6SFStEDZXMqJTt_XIaA3kerD9HqGq2-jVbvt3jyp43jl19BVoAdgFJbce3y79n_kf0J6vKwOg</addsrcrecordid><sourcetype>Open Access Repository</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>2619853140</pqid></control><display><type>article</type><title>Development of effective biotransformation process for benzohydroxamic acid production using Bacillus smithii IIIMB2907</title><source>Open Access: PubMed Central</source><source>Springer Link</source><creator>Sharma, Hitesh ; Singh, Rahul Vikram ; Ganjoo, Ananta ; Kumar, Amit ; Singh, Ravail ; Babu, Vikash</creator><creatorcontrib>Sharma, Hitesh ; Singh, Rahul Vikram ; Ganjoo, Ananta ; Kumar, Amit ; Singh, Ravail ; Babu, Vikash</creatorcontrib><description>The present study entails the usefulness of thermophilic amidase-producing bacterium in the biotransformation of benzamide to benzohydroxamic acid (BHA). A bacterium
Bacillus smithii
IIIMB2907 was isolated from a soil sample collected from hot springs of Manikaran, Himachal Pradesh, India. The whole cells of the bacterium displayed versatile substrate specificity by exhibiting significant activity with a diverse range of amides. In addition, amidase from thermophilic bacterium was induced by adding Ɛ-caprolactam in the mineral base media. The optimum temperature and pH of acyltransferase activity of amidase enzyme were found to be 50 °C and 7.0, respectively. Interestingly, half-life (
t
1/2
) of this enzyme was 17.37 h at 50 °C. Bench-scale production and purification of BHA was carried out at optimized conditions which resulted in the recovery of 64% BHA with a purity of 96%. Owing to this, the reported process in the present study can be considered of immense industrial significance for the production of BHA.</description><identifier>ISSN: 2190-572X</identifier><identifier>EISSN: 2190-5738</identifier><identifier>DOI: 10.1007/s13205-022-03109-2</identifier><identifier>PMID: 35096501</identifier><language>eng</language><publisher>Cham: Springer International Publishing</publisher><subject>Acid production ; Acyltransferase ; Agriculture ; Amidase ; Amides ; Bacillus smithii ; Benzamide ; Bioinformatics ; Biomaterials ; Biotechnology ; Biotransformation ; Cancer Research ; Caprolactam ; Chemistry ; Chemistry and Materials Science ; Enzymes ; Hot springs ; Original ; Original Article ; Stem Cells ; Substrate specificity ; Substrates ; Thermophilic bacteria</subject><ispartof>3 Biotech, 2022-02, Vol.12 (2), p.44-44, Article 44</ispartof><rights>King Abdulaziz City for Science and Technology 2022</rights><rights>King Abdulaziz City for Science and Technology 2022.</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c474t-ce0a188963cf9c4e5b766ca2346dd376236001070c0ad913f4452103ac517ffb3</citedby><cites>FETCH-LOGICAL-c474t-ce0a188963cf9c4e5b766ca2346dd376236001070c0ad913f4452103ac517ffb3</cites><orcidid>0000-0001-9784-4135</orcidid></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC8761202/pdf/$$EPDF$$P50$$Gpubmedcentral$$H</linktopdf><linktohtml>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC8761202/$$EHTML$$P50$$Gpubmedcentral$$H</linktohtml><link.rule.ids>230,314,727,780,784,885,27924,27925,53791,53793</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/35096501$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Sharma, Hitesh</creatorcontrib><creatorcontrib>Singh, Rahul Vikram</creatorcontrib><creatorcontrib>Ganjoo, Ananta</creatorcontrib><creatorcontrib>Kumar, Amit</creatorcontrib><creatorcontrib>Singh, Ravail</creatorcontrib><creatorcontrib>Babu, Vikash</creatorcontrib><title>Development of effective biotransformation process for benzohydroxamic acid production using Bacillus smithii IIIMB2907</title><title>3 Biotech</title><addtitle>3 Biotech</addtitle><addtitle>3 Biotech</addtitle><description>The present study entails the usefulness of thermophilic amidase-producing bacterium in the biotransformation of benzamide to benzohydroxamic acid (BHA). A bacterium
Bacillus smithii
IIIMB2907 was isolated from a soil sample collected from hot springs of Manikaran, Himachal Pradesh, India. The whole cells of the bacterium displayed versatile substrate specificity by exhibiting significant activity with a diverse range of amides. In addition, amidase from thermophilic bacterium was induced by adding Ɛ-caprolactam in the mineral base media. The optimum temperature and pH of acyltransferase activity of amidase enzyme were found to be 50 °C and 7.0, respectively. Interestingly, half-life (
t
1/2
) of this enzyme was 17.37 h at 50 °C. Bench-scale production and purification of BHA was carried out at optimized conditions which resulted in the recovery of 64% BHA with a purity of 96%. Owing to this, the reported process in the present study can be considered of immense industrial significance for the production of BHA.</description><subject>Acid production</subject><subject>Acyltransferase</subject><subject>Agriculture</subject><subject>Amidase</subject><subject>Amides</subject><subject>Bacillus smithii</subject><subject>Benzamide</subject><subject>Bioinformatics</subject><subject>Biomaterials</subject><subject>Biotechnology</subject><subject>Biotransformation</subject><subject>Cancer Research</subject><subject>Caprolactam</subject><subject>Chemistry</subject><subject>Chemistry and Materials Science</subject><subject>Enzymes</subject><subject>Hot springs</subject><subject>Original</subject><subject>Original Article</subject><subject>Stem Cells</subject><subject>Substrate specificity</subject><subject>Substrates</subject><subject>Thermophilic bacteria</subject><issn>2190-572X</issn><issn>2190-5738</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2022</creationdate><recordtype>article</recordtype><recordid>eNp9kUuPFCEUhYnROJNx_oALQ-LGTekFCig2Js746mSMG03cEYqCbiZV0EJV6_jrpafH9rGQDeTej3PvyUHoMYHnBEC-KIRR4A1Q2gAjoBp6D51SoqDhknX3j2_65QSdl3IN9XDCFYGH6IRxUIIDOUXfXrudG9N2cnHGyWPnvbNz2DnchzRnE4tPeTJzSBFvc7KuFFwruHfxR9rcDDl9N1Ow2Ngw7IFhsbfsUkJc44taHsel4DKFeRMCXq1WHy6oAvkIPfBmLO787j5Dn9---XT5vrn6-G51-eqqsa1s58Y6MKTrlGDWK9s63kshrKGsFcPApKBMABCQYMEMijDftpwSYMZyIr3v2Rl6edDdLv3kBlttZjPqbQ6TyTc6maD_7sSw0eu0050UhAKtAs_uBHL6urgy6ykU68bRRJeWoqmgLemUAlHRp_-g12nJsdqrFFEdZ6SFStEDZXMqJTt_XIaA3kerD9HqGq2-jVbvt3jyp43jl19BVoAdgFJbce3y79n_kf0J6vKwOg</recordid><startdate>20220201</startdate><enddate>20220201</enddate><creator>Sharma, Hitesh</creator><creator>Singh, Rahul Vikram</creator><creator>Ganjoo, Ananta</creator><creator>Kumar, Amit</creator><creator>Singh, Ravail</creator><creator>Babu, Vikash</creator><general>Springer International Publishing</general><general>Springer Nature B.V</general><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope><scope>5PM</scope><orcidid>https://orcid.org/0000-0001-9784-4135</orcidid></search><sort><creationdate>20220201</creationdate><title>Development of effective biotransformation process for benzohydroxamic acid production using Bacillus smithii IIIMB2907</title><author>Sharma, Hitesh ; Singh, Rahul Vikram ; Ganjoo, Ananta ; Kumar, Amit ; Singh, Ravail ; Babu, Vikash</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c474t-ce0a188963cf9c4e5b766ca2346dd376236001070c0ad913f4452103ac517ffb3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2022</creationdate><topic>Acid production</topic><topic>Acyltransferase</topic><topic>Agriculture</topic><topic>Amidase</topic><topic>Amides</topic><topic>Bacillus smithii</topic><topic>Benzamide</topic><topic>Bioinformatics</topic><topic>Biomaterials</topic><topic>Biotechnology</topic><topic>Biotransformation</topic><topic>Cancer Research</topic><topic>Caprolactam</topic><topic>Chemistry</topic><topic>Chemistry and Materials Science</topic><topic>Enzymes</topic><topic>Hot springs</topic><topic>Original</topic><topic>Original Article</topic><topic>Stem Cells</topic><topic>Substrate specificity</topic><topic>Substrates</topic><topic>Thermophilic bacteria</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Sharma, Hitesh</creatorcontrib><creatorcontrib>Singh, Rahul Vikram</creatorcontrib><creatorcontrib>Ganjoo, Ananta</creatorcontrib><creatorcontrib>Kumar, Amit</creatorcontrib><creatorcontrib>Singh, Ravail</creatorcontrib><creatorcontrib>Babu, Vikash</creatorcontrib><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><collection>PubMed Central (Full Participant titles)</collection><jtitle>3 Biotech</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Sharma, Hitesh</au><au>Singh, Rahul Vikram</au><au>Ganjoo, Ananta</au><au>Kumar, Amit</au><au>Singh, Ravail</au><au>Babu, Vikash</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Development of effective biotransformation process for benzohydroxamic acid production using Bacillus smithii IIIMB2907</atitle><jtitle>3 Biotech</jtitle><stitle>3 Biotech</stitle><addtitle>3 Biotech</addtitle><date>2022-02-01</date><risdate>2022</risdate><volume>12</volume><issue>2</issue><spage>44</spage><epage>44</epage><pages>44-44</pages><artnum>44</artnum><issn>2190-572X</issn><eissn>2190-5738</eissn><abstract>The present study entails the usefulness of thermophilic amidase-producing bacterium in the biotransformation of benzamide to benzohydroxamic acid (BHA). A bacterium
Bacillus smithii
IIIMB2907 was isolated from a soil sample collected from hot springs of Manikaran, Himachal Pradesh, India. The whole cells of the bacterium displayed versatile substrate specificity by exhibiting significant activity with a diverse range of amides. In addition, amidase from thermophilic bacterium was induced by adding Ɛ-caprolactam in the mineral base media. The optimum temperature and pH of acyltransferase activity of amidase enzyme were found to be 50 °C and 7.0, respectively. Interestingly, half-life (
t
1/2
) of this enzyme was 17.37 h at 50 °C. Bench-scale production and purification of BHA was carried out at optimized conditions which resulted in the recovery of 64% BHA with a purity of 96%. Owing to this, the reported process in the present study can be considered of immense industrial significance for the production of BHA.</abstract><cop>Cham</cop><pub>Springer International Publishing</pub><pmid>35096501</pmid><doi>10.1007/s13205-022-03109-2</doi><tpages>1</tpages><orcidid>https://orcid.org/0000-0001-9784-4135</orcidid><oa>free_for_read</oa></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 2190-572X |
| ispartof | 3 Biotech, 2022-02, Vol.12 (2), p.44-44, Article 44 |
| issn | 2190-572X 2190-5738 |
| language | eng |
| recordid | cdi_pubmedcentral_primary_oai_pubmedcentral_nih_gov_8761202 |
| source | Open Access: PubMed Central; Springer Link |
| subjects | Acid production Acyltransferase Agriculture Amidase Amides Bacillus smithii Benzamide Bioinformatics Biomaterials Biotechnology Biotransformation Cancer Research Caprolactam Chemistry Chemistry and Materials Science Enzymes Hot springs Original Original Article Stem Cells Substrate specificity Substrates Thermophilic bacteria |
| title | Development of effective biotransformation process for benzohydroxamic acid production using Bacillus smithii IIIMB2907 |
| url | http://sfxeu10.hosted.exlibrisgroup.com/loughborough?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2025-01-04T19%3A27%3A45IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-proquest_pubme&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=Development%20of%20effective%20biotransformation%20process%20for%20benzohydroxamic%20acid%20production%20using%20Bacillus%20smithii%20IIIMB2907&rft.jtitle=3%20Biotech&rft.au=Sharma,%20Hitesh&rft.date=2022-02-01&rft.volume=12&rft.issue=2&rft.spage=44&rft.epage=44&rft.pages=44-44&rft.artnum=44&rft.issn=2190-572X&rft.eissn=2190-5738&rft_id=info:doi/10.1007/s13205-022-03109-2&rft_dat=%3Cproquest_pubme%3E2624189906%3C/proquest_pubme%3E%3Cgrp_id%3Ecdi_FETCH-LOGICAL-c474t-ce0a188963cf9c4e5b766ca2346dd376236001070c0ad913f4452103ac517ffb3%3C/grp_id%3E%3Coa%3E%3C/oa%3E%3Curl%3E%3C/url%3E&rft_id=info:oai/&rft_pqid=2619853140&rft_id=info:pmid/35096501&rfr_iscdi=true |