Toll-like receptor 4-mediated necroptosis in the development of necrotizing enterocolitis

• Published in: Pediatric research 2022-01, Vol.91 (1), p.73-82
• Main Authors: Liu, Tianjing, Zong, Haifeng, Chen, Xiaoyu, Li, Sihang, Liu, Ziyun, Cui, Xuewei, Jia, Guoqiang, Shi, Yongyan
• Format: Article
• Language: English
• Subjects: Adaptor Proteins, Vesicular Transport - metabolism; Animals; Apoptosis; Apoptosis - physiology; Basic Science; Basic Science Article; Disease Models, Animal; Enterocolitis, Necrotizing - pathology; Female; Gastrointestinal diseases; Humans; Medicine; Medicine & Public Health; Mice; Mice, Inbred C57BL; Mice, Knockout; Necroptosis - physiology; Necrosis; Pediatric Surgery; Pediatrics; Pregnancy; Proteins; Receptor-Interacting Protein Serine-Threonine Kinases - antagonists & inhibitors; Receptors, Tumor Necrosis Factor, Type I - metabolism; Toll-Like Receptor 4 - genetics; Toll-Like Receptor 4 - physiology; Up-Regulation
• ISSN: 0031-3998; 1530-0447
• DOI: 10.1038/s41390-021-01457-y

Background Dramatic intestinal epithelial cell death leading to barrier dysfunction is one of the mechanism of neonatal necrotizing enterocolitis (NEC), in which Toll-like receptor 4 (TLR4) plays a pivotal role. This study explored the role of necroptosis, a drastic way of cell death in NEC. Methods The expression of necroptotic proteins was tested in NEC intestinal tissue and compared with controls. NEC was induced in neonatal wild-type mice and a necroptosis inhibitor was given to investigate whether NEC could be relieved. The general condition, macroscopic scoring, and histological evaluations were performed. The expression of tight junction proteins, inflammatory cytokines, and necroptosis-related proteins was measured, and barrier function was examined. Then, NEC was induced in TLR4-knockout pups to confirm the role of TLR4 in necroptosis. Results Necroptotic proteins were significantly upregulated in both NEC patient and animal models, together with the expression of TLR4. NEC could be relieved and inflammatory infiltration was decreased by necrostatin-1s. TLR4-knockout mice showed milder tissue degradation and less necroptosis after NEC induction. Conclusions Necroptosis is an essential pathological process of NEC. TLR4 may be one stimulator of necroptosis in NEC. Inhibiting the intestina l cell necroptosis might be a useful strategy in the treatment of NEC. Impact Necroptosis is a key pathological process in NEC, which appears to involve TLR4. Anti-necroptosis treatment is a promising strategy that could significantly relieve the symptoms of NEC.